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Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA.
Genes Dev. 2005 Oct 01; 19(19):2355-66.GD

Abstract

This paper shows that the small RNA MicA (previously SraD) is an antisense regulator of ompA in Escherichia coli. MicA accumulates upon entry into stationary phase and down-regulates the level of ompA mRNA. Regulation of ompA (outer membrane protein A), previously attributed to Hfq/mRNA binding, is lost upon deletion of the micA gene, whereas overexpression of MicA inhibits the synthesis of OmpA. In vitro, MicA binds to the ompA mRNA leader. Enzymatic and chemical probing was used to map the structures of MicA, the ompA mRNA leader, and the complex formed upon binding. MicA binding generates a footprint across the ompA Shine-Dalgarno sequence, consistent with a 12 + 4 base-pair interaction, which is additionally supported by the effect of mutations in vivo and by bioinformatics analysis of enterobacterial micA/ompA homolog sequences. MicA is conserved in many enterobacteria, as is its ompA target site. In vitro toeprinting confirmed that binding of MicA specifically interferes with ribosome binding. We propose that MicA, when present at high levels, blocks ribosome binding at the ompA translation start site, which-in line with previous work-secondarily facilitates RNase E cleavage and subsequent mRNA decay. MicA requires the presence of the Hfq protein, although the mechanistic basis for this remains unclear.

Authors+Show Affiliations

Department of Cell and Molecular Biology, Uppsala University, S-75124 Uppsala, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16204185

Citation

Udekwu, Klas I., et al. "Hfq-dependent Regulation of OmpA Synthesis Is Mediated By an Antisense RNA." Genes & Development, vol. 19, no. 19, 2005, pp. 2355-66.
Udekwu KI, Darfeuille F, Vogel J, et al. Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA. Genes Dev. 2005;19(19):2355-66.
Udekwu, K. I., Darfeuille, F., Vogel, J., Reimegård, J., Holmqvist, E., & Wagner, E. G. (2005). Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA. Genes & Development, 19(19), 2355-66.
Udekwu KI, et al. Hfq-dependent Regulation of OmpA Synthesis Is Mediated By an Antisense RNA. Genes Dev. 2005 Oct 1;19(19):2355-66. PubMed PMID: 16204185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA. AU - Udekwu,Klas I, AU - Darfeuille,Fabien, AU - Vogel,Jörg, AU - Reimegård,Johan, AU - Holmqvist,Erik, AU - Wagner,E Gerhart H, PY - 2005/10/6/pubmed PY - 2005/12/13/medline PY - 2005/10/6/entrez SP - 2355 EP - 66 JF - Genes & development JO - Genes Dev VL - 19 IS - 19 N2 - This paper shows that the small RNA MicA (previously SraD) is an antisense regulator of ompA in Escherichia coli. MicA accumulates upon entry into stationary phase and down-regulates the level of ompA mRNA. Regulation of ompA (outer membrane protein A), previously attributed to Hfq/mRNA binding, is lost upon deletion of the micA gene, whereas overexpression of MicA inhibits the synthesis of OmpA. In vitro, MicA binds to the ompA mRNA leader. Enzymatic and chemical probing was used to map the structures of MicA, the ompA mRNA leader, and the complex formed upon binding. MicA binding generates a footprint across the ompA Shine-Dalgarno sequence, consistent with a 12 + 4 base-pair interaction, which is additionally supported by the effect of mutations in vivo and by bioinformatics analysis of enterobacterial micA/ompA homolog sequences. MicA is conserved in many enterobacteria, as is its ompA target site. In vitro toeprinting confirmed that binding of MicA specifically interferes with ribosome binding. We propose that MicA, when present at high levels, blocks ribosome binding at the ompA translation start site, which-in line with previous work-secondarily facilitates RNase E cleavage and subsequent mRNA decay. MicA requires the presence of the Hfq protein, although the mechanistic basis for this remains unclear. SN - 0890-9369 UR - https://www.unboundmedicine.com/medline/citation/16204185/Hfq_dependent_regulation_of_OmpA_synthesis_is_mediated_by_an_antisense_RNA_ L2 - http://www.genesdev.org/cgi/pmidlookup?view=long&pmid=16204185 DB - PRIME DP - Unbound Medicine ER -