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Increase in bone mass and bone strength by Sambucus williamsii HANCE in ovariectomized rats.

Abstract

Herbal Sambucus williamsii HANCE (SWH) is a folk medicine with a long history of safe use for treatment of bone fractures and joint diseases in China. The present study was designed to investigate if SWH extract could be used for treatment of postmenopausal osteoporosis. SWH extracts (30 or 60 mg/100 g body weight/d) were orally administrated to four-months-old ovariectomized (OVX) rats for 3 months. SWH extracts did not alter weight gain and uterus weight in OVX rats. SWH extracts significantly increased serum Ca levels (p<0.05, vs. OVX control group) as well as decreased urinary Ca excretion (p<0.01, vs. OVX control group) in OVX rats. The upregulation of serum alkaline phosphatase, serum osteocalcin as well as urinary deoxypyridinoline levels by OVX was suppressed by treatment with SWH extracts in rats (p<0.05, vs. OVX control group). SWH extract increased the stiffness of femur at both dosage (p<0.05, vs. OVX control group) and increased tibial bone mineral density at 60 mg/100 g body weight/d (p<0.05, vs. OVX control group) in OVX rats. Our results indicate that orally administrated SWH extracts can decrease urinary calcium excretion and bone turnover rate in OVX rats, resulting in positive effects on biomechanical strength of bone and bone mineral density. This study is the first to report that SWH could be considered as a potential candidate for management of postmenopausal osteoporosis. Then in vitro experiments were performed to determine the potential molecular mechanism of the anti-osteoporotic effect of SWH. Results suggested that chloroform fraction and ethyl acetate fraction of SWH can inhibit osteoclastogenesis osteoclast by modulating the expression of osteoprotegrin (OPG) and receptor activator of NF-kappaB ligand (RANKL) mRNA in osteoblastic UMR 106 cells. Both of them increased OPG mRNA and decreased RANKL mRNA expression, resulting in a dose-dependent increase in OPG/RANKL mRNA ratio (p<0.01, vs. vehicle-treated). Taken together, SWH treatment can effectively suppress the OVX-induced increase in bone turnover and its effects might be mediated by a decrease in osteoclastogenesis.

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  • Authors+Show Affiliations

    ,

    Department of Pharmacology, Shenyang Pharmaceutical University; Shenyang 110016, China.

    , , , , ,

    Source

    Biological & pharmaceutical bulletin 28:10 2005 Oct pg 1879-85

    MeSH

    Animals
    Base Sequence
    Biomechanical Phenomena
    Body Weight
    Bone and Bones
    Carrier Proteins
    Cell Line
    DNA Primers
    Female
    Glycoproteins
    Membrane Glycoproteins
    Organ Size
    Osteoporosis
    Osteoprotegerin
    Ovariectomy
    RANK Ligand
    RNA, Messenger
    Rats
    Rats, Sprague-Dawley
    Receptors, Cytoplasmic and Nuclear
    Receptors, Tumor Necrosis Factor
    Reverse Transcriptase Polymerase Chain Reaction
    Uterus

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    16204939