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Increased hepatocyte fas expression and apoptosis in HIV and hepatitis C virus coinfection.
J Infect Dis. 2005 Nov 01; 192(9):1566-76.JI

Abstract

BACKGROUND

Chronic hepatitis C disease (CHC) follows an accelerated course in human immunodeficiency virus (HIV) coinfection. The reasons for this are unclear. Fas-mediated hepatocyte apoptosis is involved in the pathogenesis of hepatitis C virus (HCV) infection. We sought to compare the expression of Fas on hepatocytes and irreversible apoptosis of hepatocytes among patients with CHC with and without HCV/HIV coinfection.

METHODS

Fas-immunostained hepatocytes were semiquantified, and apoptotic hepatocytes were detected by staining caspase-cleaved cytokeratin 18 filaments and counted across the entire section of liver-biopsy specimens from HCV-infected patients with and without HCV/HIV coinfection.

RESULTS

One hundred thirty-four HCV/HIV-coinfected and 100 HCV-infected patients were included. HCV/HIV coinfection was associated with both diffuse distribution of Fas-stained hepatocytes (adjusted odds ratio [AOR], 7.4 [95% confidence interval {CI}, 3.8-14.4]) and with apoptotic hepatocyte counts greater than the median (AOR, 2.5 [95% CI, 1.5-4.5]). In HCV/HIV-coinfected patients, CD4+ cell nadir<200 cells/mL was associated with both Fas expression (AOR, 2.9 [95% CI, 1.3-6.8]) and hepatocyte apoptosis (AOR, 2.3 [95% CI, 1.1-4.9]).

CONCLUSION

HCV/HIV-coinfected patients show higher levels of hepatocytes expressing Fas and undergoing irreversible apoptosis than do HCV-infected patients. However, low CD4+ cell nadirs in coinfected patients are associated with hepatocyte Fas expression and apoptosis.

Authors+Show Affiliations

Servicio de Medicina Interna, Hospital Universitario de Valme, Seville, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16206071

Citation

Macias, Juan, et al. "Increased Hepatocyte Fas Expression and Apoptosis in HIV and Hepatitis C Virus Coinfection." The Journal of Infectious Diseases, vol. 192, no. 9, 2005, pp. 1566-76.
Macias J, Japón MA, Sáez C, et al. Increased hepatocyte fas expression and apoptosis in HIV and hepatitis C virus coinfection. J Infect Dis. 2005;192(9):1566-76.
Macias, J., Japón, M. A., Sáez, C., Palacios, R. B., Mira, J. A., García-García, J. A., Merchante, N., Vergara, S., Lozano, F., Gómez-Mateos, J., & Pineda, J. A. (2005). Increased hepatocyte fas expression and apoptosis in HIV and hepatitis C virus coinfection. The Journal of Infectious Diseases, 192(9), 1566-76.
Macias J, et al. Increased Hepatocyte Fas Expression and Apoptosis in HIV and Hepatitis C Virus Coinfection. J Infect Dis. 2005 Nov 1;192(9):1566-76. PubMed PMID: 16206071.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased hepatocyte fas expression and apoptosis in HIV and hepatitis C virus coinfection. AU - Macias,Juan, AU - Japón,Miguel A, AU - Sáez,Carmen, AU - Palacios,Rosa B, AU - Mira,José A, AU - García-García,José A, AU - Merchante,Nicolás, AU - Vergara,Salvador, AU - Lozano,Fernando, AU - Gómez-Mateos,Jesús, AU - Pineda,Juan A, Y1 - 2005/09/29/ PY - 2004/11/24/received PY - 2005/05/02/accepted PY - 2005/10/6/pubmed PY - 2006/3/17/medline PY - 2005/10/6/entrez SP - 1566 EP - 76 JF - The Journal of infectious diseases JO - J Infect Dis VL - 192 IS - 9 N2 - BACKGROUND: Chronic hepatitis C disease (CHC) follows an accelerated course in human immunodeficiency virus (HIV) coinfection. The reasons for this are unclear. Fas-mediated hepatocyte apoptosis is involved in the pathogenesis of hepatitis C virus (HCV) infection. We sought to compare the expression of Fas on hepatocytes and irreversible apoptosis of hepatocytes among patients with CHC with and without HCV/HIV coinfection. METHODS: Fas-immunostained hepatocytes were semiquantified, and apoptotic hepatocytes were detected by staining caspase-cleaved cytokeratin 18 filaments and counted across the entire section of liver-biopsy specimens from HCV-infected patients with and without HCV/HIV coinfection. RESULTS: One hundred thirty-four HCV/HIV-coinfected and 100 HCV-infected patients were included. HCV/HIV coinfection was associated with both diffuse distribution of Fas-stained hepatocytes (adjusted odds ratio [AOR], 7.4 [95% confidence interval {CI}, 3.8-14.4]) and with apoptotic hepatocyte counts greater than the median (AOR, 2.5 [95% CI, 1.5-4.5]). In HCV/HIV-coinfected patients, CD4+ cell nadir<200 cells/mL was associated with both Fas expression (AOR, 2.9 [95% CI, 1.3-6.8]) and hepatocyte apoptosis (AOR, 2.3 [95% CI, 1.1-4.9]). CONCLUSION: HCV/HIV-coinfected patients show higher levels of hepatocytes expressing Fas and undergoing irreversible apoptosis than do HCV-infected patients. However, low CD4+ cell nadirs in coinfected patients are associated with hepatocyte Fas expression and apoptosis. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/16206071/Increased_hepatocyte_fas_expression_and_apoptosis_in_HIV_and_hepatitis_C_virus_coinfection_ DB - PRIME DP - Unbound Medicine ER -