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Study of the mass spectrometric fragmentation of pseudouridine: comparison of fragmentation data obtained by matrix-assisted laser desorption/ionisation post-source decay, electrospray ion trap multistage mass spectrometry, and by a method utilising electrospray quadrupole time-of-flight tandem mass spectrometry and in-source fragmentation.
Rapid Commun Mass Spectrom. 2005; 19(21):3075-85.RC

Abstract

Many nucleosides and their modified forms have been studied by mass spectrometry elaborating the detailed fragmentation pathways under MS2 and MS(n) conditions. Although the C-nucleoside pseudouridine has been fragmented and studied briefly, usually amongst many other nucleosides, it has not been investigated to the same extent as other nucleosides. In this report a number of different mass spectrometric techniques are applied to obtain a fuller picture of pseudouridine fragmentation. At the same time this study is used to compare different tandem mass spectrometric techniques, including a novel methodology utilising a quadrupole time-of-flight (Q-ToF) instrument for MS(n) analysis comparable with that available with an ion trap mass spectrometer.

Authors+Show Affiliations

Biomolecular Analysis Mass Spectrometry (BAMS) Facility, Grove Building, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK. E.dudley@swansea.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16206154

Citation

Dudley, Edward, et al. "Study of the Mass Spectrometric Fragmentation of Pseudouridine: Comparison of Fragmentation Data Obtained By Matrix-assisted Laser Desorption/ionisation Post-source Decay, Electrospray Ion Trap Multistage Mass Spectrometry, and By a Method Utilising Electrospray Quadrupole Time-of-flight Tandem Mass Spectrometry and In-source Fragmentation." Rapid Communications in Mass Spectrometry : RCM, vol. 19, no. 21, 2005, pp. 3075-85.
Dudley E, Tuytten R, Bond A, et al. Study of the mass spectrometric fragmentation of pseudouridine: comparison of fragmentation data obtained by matrix-assisted laser desorption/ionisation post-source decay, electrospray ion trap multistage mass spectrometry, and by a method utilising electrospray quadrupole time-of-flight tandem mass spectrometry and in-source fragmentation. Rapid Commun Mass Spectrom. 2005;19(21):3075-85.
Dudley, E., Tuytten, R., Bond, A., Lemière, F., Brenton, A. G., Esmans, E. L., & Newton, R. P. (2005). Study of the mass spectrometric fragmentation of pseudouridine: comparison of fragmentation data obtained by matrix-assisted laser desorption/ionisation post-source decay, electrospray ion trap multistage mass spectrometry, and by a method utilising electrospray quadrupole time-of-flight tandem mass spectrometry and in-source fragmentation. Rapid Communications in Mass Spectrometry : RCM, 19(21), 3075-85.
Dudley E, et al. Study of the Mass Spectrometric Fragmentation of Pseudouridine: Comparison of Fragmentation Data Obtained By Matrix-assisted Laser Desorption/ionisation Post-source Decay, Electrospray Ion Trap Multistage Mass Spectrometry, and By a Method Utilising Electrospray Quadrupole Time-of-flight Tandem Mass Spectrometry and In-source Fragmentation. Rapid Commun Mass Spectrom. 2005;19(21):3075-85. PubMed PMID: 16206154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Study of the mass spectrometric fragmentation of pseudouridine: comparison of fragmentation data obtained by matrix-assisted laser desorption/ionisation post-source decay, electrospray ion trap multistage mass spectrometry, and by a method utilising electrospray quadrupole time-of-flight tandem mass spectrometry and in-source fragmentation. AU - Dudley,Edward, AU - Tuytten,Robin, AU - Bond,Alison, AU - Lemière,Filip, AU - Brenton,A Gareth, AU - Esmans,Eddy L, AU - Newton,Russell P, PY - 2005/10/6/pubmed PY - 2005/12/15/medline PY - 2005/10/6/entrez SP - 3075 EP - 85 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 19 IS - 21 N2 - Many nucleosides and their modified forms have been studied by mass spectrometry elaborating the detailed fragmentation pathways under MS2 and MS(n) conditions. Although the C-nucleoside pseudouridine has been fragmented and studied briefly, usually amongst many other nucleosides, it has not been investigated to the same extent as other nucleosides. In this report a number of different mass spectrometric techniques are applied to obtain a fuller picture of pseudouridine fragmentation. At the same time this study is used to compare different tandem mass spectrometric techniques, including a novel methodology utilising a quadrupole time-of-flight (Q-ToF) instrument for MS(n) analysis comparable with that available with an ion trap mass spectrometer. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/16206154/Study_of_the_mass_spectrometric_fragmentation_of_pseudouridine:_comparison_of_fragmentation_data_obtained_by_matrix_assisted_laser_desorption/ionisation_post_source_decay_electrospray_ion_trap_multistage_mass_spectrometry_and_by_a_method_utilising_electrospray_quadrupole_time_of_flight_tandem_mass_spectrometry_and_in_source_fragmentation_ L2 - https://doi.org/10.1002/rcm.2151 DB - PRIME DP - Unbound Medicine ER -