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Comparative effects of 2 antioxidants, selenomethionine and epigallocatechin-gallate, on catabolic and anabolic gene expression of articular chondrocytes.
J Rheumatol. 2005 Oct; 32(10):1958-67.JR

Abstract

OBJECTIVE

. To determine the effects of selenomethionine (Se-met) and epigallocatechin-gallate (EGCg) on gene expression, activation of mitogen-activating kinases, and DNA binding of nuclear factor-kappaB (NF-kappaB) and apolipoprotein-1 (AP-1) in articular chondrocytes.

METHODS

Chondrocytes, cultured in low-oxygen tension, were pretreated with L-selenomethionine or EGCg for 24 h, followed by interleukin 1 (IL-1beta) for 1 h (nuclear and cytoplasmic extracts) or 24 h (RNA extraction). Reverse transcription-polymerase chain reaction was performed to determine mRNA levels of matrix metalloproteinases (MMP-1, -3, -13), aggrecanases (-1, -2), IL-1beta, inducible nitric oxide synthase, cyclooxygenases (-1, -2), type II collagen and aggrecan, and transforming growth factor-beta (TGF-beta1, -2, -3) and their receptors I and II. Activity of mitogen-activating protein kinases (MAPK) was assayed by Western blot and AP-1/NF-kB DNA binding by electrophoretic mobility shift assay.

RESULTS

Pretreatment with 0.5 microM Se-met prevented IL-1beta-induced MMP-1 and aggrecanase-1 expression, and reduced the cytokine inhibitory effect on type II collagen, aggrecan core protein, and TGF-beta receptor II (TGF-betaRII) mRNA levels. EGCg was more efficient in modulating the effects of IL-1beta on the genes studied. Whereas EGCg inhibited the IL-1beta-activated MAPK, NF-kappaB, and AP-1, Se-met stimulated that signaling pathway. This could account for the differential effects exerted by these antioxidants on chondrocytes.

CONCLUSION

Our data provide insights into the mechanisms whereby ECGg and selenium modulate chondrocyte metabolism. Despite their differential mechanisms of action, the 2 compounds may exert global beneficial effects on articular cartilage.

Authors+Show Affiliations

Laboratory of Connective Tissue Biochemistry, Faculty of Medicine, 14032 Caen Cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16206353

Citation

Andriamanalijaona, Rina, et al. "Comparative Effects of 2 Antioxidants, Selenomethionine and Epigallocatechin-gallate, On Catabolic and Anabolic Gene Expression of Articular Chondrocytes." The Journal of Rheumatology, vol. 32, no. 10, 2005, pp. 1958-67.
Andriamanalijaona R, Kypriotou M, Baugé C, et al. Comparative effects of 2 antioxidants, selenomethionine and epigallocatechin-gallate, on catabolic and anabolic gene expression of articular chondrocytes. J Rheumatol. 2005;32(10):1958-67.
Andriamanalijaona, R., Kypriotou, M., Baugé, C., Renard, E., Legendre, F., Raoudi, M., Boumediene, K., Gatto, H., Monginoux, P., & Pujol, J. P. (2005). Comparative effects of 2 antioxidants, selenomethionine and epigallocatechin-gallate, on catabolic and anabolic gene expression of articular chondrocytes. The Journal of Rheumatology, 32(10), 1958-67.
Andriamanalijaona R, et al. Comparative Effects of 2 Antioxidants, Selenomethionine and Epigallocatechin-gallate, On Catabolic and Anabolic Gene Expression of Articular Chondrocytes. J Rheumatol. 2005;32(10):1958-67. PubMed PMID: 16206353.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative effects of 2 antioxidants, selenomethionine and epigallocatechin-gallate, on catabolic and anabolic gene expression of articular chondrocytes. AU - Andriamanalijaona,Rina, AU - Kypriotou,Madeleine, AU - Baugé,Catherine, AU - Renard,Emmanuelle, AU - Legendre,Florence, AU - Raoudi,Maha, AU - Boumediene,Karim, AU - Gatto,Hugues, AU - Monginoux,Patricia, AU - Pujol,Jean-Pierre, PY - 2005/10/6/pubmed PY - 2005/12/15/medline PY - 2005/10/6/entrez SP - 1958 EP - 67 JF - The Journal of rheumatology JO - J. Rheumatol. VL - 32 IS - 10 N2 - OBJECTIVE: . To determine the effects of selenomethionine (Se-met) and epigallocatechin-gallate (EGCg) on gene expression, activation of mitogen-activating kinases, and DNA binding of nuclear factor-kappaB (NF-kappaB) and apolipoprotein-1 (AP-1) in articular chondrocytes. METHODS: Chondrocytes, cultured in low-oxygen tension, were pretreated with L-selenomethionine or EGCg for 24 h, followed by interleukin 1 (IL-1beta) for 1 h (nuclear and cytoplasmic extracts) or 24 h (RNA extraction). Reverse transcription-polymerase chain reaction was performed to determine mRNA levels of matrix metalloproteinases (MMP-1, -3, -13), aggrecanases (-1, -2), IL-1beta, inducible nitric oxide synthase, cyclooxygenases (-1, -2), type II collagen and aggrecan, and transforming growth factor-beta (TGF-beta1, -2, -3) and their receptors I and II. Activity of mitogen-activating protein kinases (MAPK) was assayed by Western blot and AP-1/NF-kB DNA binding by electrophoretic mobility shift assay. RESULTS: Pretreatment with 0.5 microM Se-met prevented IL-1beta-induced MMP-1 and aggrecanase-1 expression, and reduced the cytokine inhibitory effect on type II collagen, aggrecan core protein, and TGF-beta receptor II (TGF-betaRII) mRNA levels. EGCg was more efficient in modulating the effects of IL-1beta on the genes studied. Whereas EGCg inhibited the IL-1beta-activated MAPK, NF-kappaB, and AP-1, Se-met stimulated that signaling pathway. This could account for the differential effects exerted by these antioxidants on chondrocytes. CONCLUSION: Our data provide insights into the mechanisms whereby ECGg and selenium modulate chondrocyte metabolism. Despite their differential mechanisms of action, the 2 compounds may exert global beneficial effects on articular cartilage. SN - 0315-162X UR - https://www.unboundmedicine.com/medline/citation/16206353/Comparative_effects_of_2_antioxidants_selenomethionine_and_epigallocatechin_gallate_on_catabolic_and_anabolic_gene_expression_of_articular_chondrocytes_ L2 - http://www.jrheum.org/cgi/pmidlookup?view=long&pmid=16206353 DB - PRIME DP - Unbound Medicine ER -