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Antibacterial properties of Ag nanoparticle loaded multilayers and formation of magnetically directed antibacterial microparticles.
Langmuir. 2005 Oct 11; 21(21):9651-9.L

Abstract

Antibacterial coatings based on hydrogen-bonded multilayers containing in situ synthesized Ag nanoparticles were created on planar surfaces and on magnetic colloidal particles. We report the antibacterial properties of these coatings, determined using a disk-diffusion (Kirby-Bauer) test, as a function of the film thickness and the concentration of Ag nanoparticles in the hydrogen-bonded multilayers. The zone of inhibition (ZoI) determined by the disk-diffusion test increases as the thickness of the multilayer film is increased. Results obtained for the values of the ZoI as a function of film thickness can be described adequately with a simple diffusion model (i.e., the square of the zone of inhibition (ZoI) depended linearly on the logarithm of the thickness of the silver-loaded films). This observation suggests that, in order to incrementally increase the ZoI, an exponentially increasing amount of Ag is required within the multilayers. In general, there was no statistically significant correlation between the zone of inhibition and the number of Ag loading and reduction cycles. The duration of sustained release of antibacterial Ag ions from these coatings, however, could be prolonged by increasing the total supply of zerovalent silver in the films via multiple loading and reduction cycles. These results indicate that the release of silver is controlled by an oxidation mechanism at the surface of the nanoparticles and that repeated loading and reduction of silver leads preferentially to growth of the existing silver nanoparticles in the film as opposed to nucleation of new Ag nanoparticles. We also show that magnetic microspheres coated with silver nanoparticle loaded hydrogen-bonded multilayer thin films can be used to deliver antibacterial agents to specific locations. The minimum inhibitory concentration (MIC) of nanocomposite coated microspheres was determined by the agar dilution technique: antibacterial magnetic microspheres with higher concentrations of Ag nanoparticles exhibited lower MIC values.

Authors+Show Affiliations

Department of Chemical Engineering and the Center for Materials Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16207049

Citation

Lee, Daeyeon, et al. "Antibacterial Properties of Ag Nanoparticle Loaded Multilayers and Formation of Magnetically Directed Antibacterial Microparticles." Langmuir : the ACS Journal of Surfaces and Colloids, vol. 21, no. 21, 2005, pp. 9651-9.
Lee D, Cohen RE, Rubner MF. Antibacterial properties of Ag nanoparticle loaded multilayers and formation of magnetically directed antibacterial microparticles. Langmuir. 2005;21(21):9651-9.
Lee, D., Cohen, R. E., & Rubner, M. F. (2005). Antibacterial properties of Ag nanoparticle loaded multilayers and formation of magnetically directed antibacterial microparticles. Langmuir : the ACS Journal of Surfaces and Colloids, 21(21), 9651-9.
Lee D, Cohen RE, Rubner MF. Antibacterial Properties of Ag Nanoparticle Loaded Multilayers and Formation of Magnetically Directed Antibacterial Microparticles. Langmuir. 2005 Oct 11;21(21):9651-9. PubMed PMID: 16207049.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial properties of Ag nanoparticle loaded multilayers and formation of magnetically directed antibacterial microparticles. AU - Lee,Daeyeon, AU - Cohen,Robert E, AU - Rubner,Michael F, PY - 2005/10/7/pubmed PY - 2007/4/11/medline PY - 2005/10/7/entrez SP - 9651 EP - 9 JF - Langmuir : the ACS journal of surfaces and colloids JO - Langmuir VL - 21 IS - 21 N2 - Antibacterial coatings based on hydrogen-bonded multilayers containing in situ synthesized Ag nanoparticles were created on planar surfaces and on magnetic colloidal particles. We report the antibacterial properties of these coatings, determined using a disk-diffusion (Kirby-Bauer) test, as a function of the film thickness and the concentration of Ag nanoparticles in the hydrogen-bonded multilayers. The zone of inhibition (ZoI) determined by the disk-diffusion test increases as the thickness of the multilayer film is increased. Results obtained for the values of the ZoI as a function of film thickness can be described adequately with a simple diffusion model (i.e., the square of the zone of inhibition (ZoI) depended linearly on the logarithm of the thickness of the silver-loaded films). This observation suggests that, in order to incrementally increase the ZoI, an exponentially increasing amount of Ag is required within the multilayers. In general, there was no statistically significant correlation between the zone of inhibition and the number of Ag loading and reduction cycles. The duration of sustained release of antibacterial Ag ions from these coatings, however, could be prolonged by increasing the total supply of zerovalent silver in the films via multiple loading and reduction cycles. These results indicate that the release of silver is controlled by an oxidation mechanism at the surface of the nanoparticles and that repeated loading and reduction of silver leads preferentially to growth of the existing silver nanoparticles in the film as opposed to nucleation of new Ag nanoparticles. We also show that magnetic microspheres coated with silver nanoparticle loaded hydrogen-bonded multilayer thin films can be used to deliver antibacterial agents to specific locations. The minimum inhibitory concentration (MIC) of nanocomposite coated microspheres was determined by the agar dilution technique: antibacterial magnetic microspheres with higher concentrations of Ag nanoparticles exhibited lower MIC values. SN - 0743-7463 UR - https://www.unboundmedicine.com/medline/citation/16207049/Antibacterial_properties_of_Ag_nanoparticle_loaded_multilayers_and_formation_of_magnetically_directed_antibacterial_microparticles_ L2 - https://doi.org/10.1021/la0513306 DB - PRIME DP - Unbound Medicine ER -