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Antibiotic susceptabilities of Pseudomonas aeruginosa isolates derived from patients with cystic fibrosis under aerobic, anaerobic, and biofilm conditions.
J Clin Microbiol. 2005 Oct; 43(10):5085-90.JC

Abstract

Recent studies have determined that Pseudomonas aeruginosa can live in a biofilm mode within hypoxic mucus in the airways of patients with cystic fibrosis (CF). P. aeruginosa grown under anaerobic and biofilm conditions may better approximate in vivo growth conditions in the CF airways, and combination antibiotic susceptibility testing of anaerobically and biofilm-grown isolates may be more relevant than traditional susceptibility testing under planktonic aerobic conditions. We tested 16 multidrug-resistant isolates of P. aeruginosa derived from CF patients using multiple combination bactericidal testing to compare the efficacies of double and triple antibiotic combinations against the isolates grown under traditional aerobic planktonic conditions, in planktonic anaerobic conditions, and in biofilm mode. Both anaerobically grown and biofilm-grown bacteria were significantly less susceptible (P < 0.01) to single and combination antibiotics than corresponding aerobic planktonically grown isolates. Furthermore, the antibiotic combinations that were bactericidal under anaerobic conditions were often different from those that were bactericidal against the same organisms grown as biofilms. The most effective combinations under all conditions were colistin (tested at concentrations suitable for nebulization) either alone or in combination with tobramycin (10 microg ml(-1)), followed by meropenem combined with tobramycin or ciprofloxacin. The findings of this study illustrate that antibiotic sensitivities are dependent on culture conditions and highlight the complexities of choosing appropriate combination therapy for multidrug-resistant P. aeruginosa in the CF lung.

Authors+Show Affiliations

Department of Infectious Diseases and Immunology, University of Sydney, Sydney, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16207967

Citation

Hill, Dominic, et al. "Antibiotic Susceptabilities of Pseudomonas Aeruginosa Isolates Derived From Patients With Cystic Fibrosis Under Aerobic, Anaerobic, and Biofilm Conditions." Journal of Clinical Microbiology, vol. 43, no. 10, 2005, pp. 5085-90.
Hill D, Rose B, Pajkos A, et al. Antibiotic susceptabilities of Pseudomonas aeruginosa isolates derived from patients with cystic fibrosis under aerobic, anaerobic, and biofilm conditions. J Clin Microbiol. 2005;43(10):5085-90.
Hill, D., Rose, B., Pajkos, A., Robinson, M., Bye, P., Bell, S., Elkins, M., Thompson, B., Macleod, C., Aaron, S. D., & Harbour, C. (2005). Antibiotic susceptabilities of Pseudomonas aeruginosa isolates derived from patients with cystic fibrosis under aerobic, anaerobic, and biofilm conditions. Journal of Clinical Microbiology, 43(10), 5085-90.
Hill D, et al. Antibiotic Susceptabilities of Pseudomonas Aeruginosa Isolates Derived From Patients With Cystic Fibrosis Under Aerobic, Anaerobic, and Biofilm Conditions. J Clin Microbiol. 2005;43(10):5085-90. PubMed PMID: 16207967.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibiotic susceptabilities of Pseudomonas aeruginosa isolates derived from patients with cystic fibrosis under aerobic, anaerobic, and biofilm conditions. AU - Hill,Dominic, AU - Rose,Barbara, AU - Pajkos,Aniko, AU - Robinson,Michael, AU - Bye,Peter, AU - Bell,Scott, AU - Elkins,Mark, AU - Thompson,Barbara, AU - Macleod,Colin, AU - Aaron,Shawn D, AU - Harbour,Colin, PY - 2005/10/7/pubmed PY - 2005/12/13/medline PY - 2005/10/7/entrez SP - 5085 EP - 90 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 43 IS - 10 N2 - Recent studies have determined that Pseudomonas aeruginosa can live in a biofilm mode within hypoxic mucus in the airways of patients with cystic fibrosis (CF). P. aeruginosa grown under anaerobic and biofilm conditions may better approximate in vivo growth conditions in the CF airways, and combination antibiotic susceptibility testing of anaerobically and biofilm-grown isolates may be more relevant than traditional susceptibility testing under planktonic aerobic conditions. We tested 16 multidrug-resistant isolates of P. aeruginosa derived from CF patients using multiple combination bactericidal testing to compare the efficacies of double and triple antibiotic combinations against the isolates grown under traditional aerobic planktonic conditions, in planktonic anaerobic conditions, and in biofilm mode. Both anaerobically grown and biofilm-grown bacteria were significantly less susceptible (P < 0.01) to single and combination antibiotics than corresponding aerobic planktonically grown isolates. Furthermore, the antibiotic combinations that were bactericidal under anaerobic conditions were often different from those that were bactericidal against the same organisms grown as biofilms. The most effective combinations under all conditions were colistin (tested at concentrations suitable for nebulization) either alone or in combination with tobramycin (10 microg ml(-1)), followed by meropenem combined with tobramycin or ciprofloxacin. The findings of this study illustrate that antibiotic sensitivities are dependent on culture conditions and highlight the complexities of choosing appropriate combination therapy for multidrug-resistant P. aeruginosa in the CF lung. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/16207967/Antibiotic_susceptabilities_of_Pseudomonas_aeruginosa_isolates_derived_from_patients_with_cystic_fibrosis_under_aerobic_anaerobic_and_biofilm_conditions_ L2 - https://journals.asm.org/doi/10.1128/JCM.43.10.5085-5090.2005?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -