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99mTc ovalbumin labelled eggs for gastric emptying scintigraphy: in-vitro comparison of solid food markers.
Nucl Med Commun. 2005 Nov; 26(11):1021-5.NM

Abstract

BACKGROUND

The reliability of solid phase gastric emptying measurements by scintigraphy requires a marker that remains within the solid component of the test meal, and which is not degraded by the gastric juice throughout the scintigraphic procedure. In Europe, foods are most often labelled with 99mTc rhenium sulfide macrocolloid (RSMC) but this solid phase marker was withdrawn from the market in January 2004.

OBJECTIVE

To test other potential solid phase markers and to compare them to the reference marker RSMC. These markers were rhenium sulfide nanocolloid (RSNC), tin fluoride colloid (TFC), phytates and two albumins (Alb and AlbC). All were radiolabelled with 99mTc.

METHODS

After quality control, each 99mTc marker was incorporated into the albumin of one egg. Then, egg white and yolk were mixed together, and a well-cooked omelette was prepared. Aliquots of the omelette were incubated with an acidic solution of pepsin at 37 degrees C which mimicked gastric juice. Unbound radioactivity in the supernatant fraction was measured at various times up to 3 h.

RESULTS

The radiochemical purity was > 95% for all radiopharmaceuticals. During the in-vitro incubation, the percentage of 99mTc labelled colloids released from the omelette increased continuously: after 3 h, 5% for TFC and RSMC, 8% for phytates, and > 9% for the two albumins and RSNC.

CONCLUSION

Considering quality controls and release of 99mTc during in-vitro incubation of the omelette, TFC showed the same behaviour as the reference marker RSMC. Thus, TFC seems to be the best candidate to replace RSMC for the radiolabelling of the solid phase of the gastric emptying test meal.

Authors+Show Affiliations

Nuclear Medicine Department, University Hospital, Brest, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16208181

Citation

Blanc, Frédérique, et al. "99mTc Ovalbumin Labelled Eggs for Gastric Emptying Scintigraphy: In-vitro Comparison of Solid Food Markers." Nuclear Medicine Communications, vol. 26, no. 11, 2005, pp. 1021-5.
Blanc F, Salaun PY, Couturier O, et al. 99mTc ovalbumin labelled eggs for gastric emptying scintigraphy: in-vitro comparison of solid food markers. Nucl Med Commun. 2005;26(11):1021-5.
Blanc, F., Salaun, P. Y., Couturier, O., Querellou, S., Le Duc-Pennec, A., Mougin-Degraef, M., Bizais, Y., & Legendre, J. M. (2005). 99mTc ovalbumin labelled eggs for gastric emptying scintigraphy: in-vitro comparison of solid food markers. Nuclear Medicine Communications, 26(11), 1021-5.
Blanc F, et al. 99mTc Ovalbumin Labelled Eggs for Gastric Emptying Scintigraphy: In-vitro Comparison of Solid Food Markers. Nucl Med Commun. 2005;26(11):1021-5. PubMed PMID: 16208181.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 99mTc ovalbumin labelled eggs for gastric emptying scintigraphy: in-vitro comparison of solid food markers. AU - Blanc,Frédérique, AU - Salaun,Pierre Y, AU - Couturier,Olivier, AU - Querellou,Solène, AU - Le Duc-Pennec,Alexandra, AU - Mougin-Degraef,Marie, AU - Bizais,Yves, AU - Legendre,Jean M, PY - 2005/10/7/pubmed PY - 2006/2/28/medline PY - 2005/10/7/entrez SP - 1021 EP - 5 JF - Nuclear medicine communications JO - Nucl Med Commun VL - 26 IS - 11 N2 - BACKGROUND: The reliability of solid phase gastric emptying measurements by scintigraphy requires a marker that remains within the solid component of the test meal, and which is not degraded by the gastric juice throughout the scintigraphic procedure. In Europe, foods are most often labelled with 99mTc rhenium sulfide macrocolloid (RSMC) but this solid phase marker was withdrawn from the market in January 2004. OBJECTIVE: To test other potential solid phase markers and to compare them to the reference marker RSMC. These markers were rhenium sulfide nanocolloid (RSNC), tin fluoride colloid (TFC), phytates and two albumins (Alb and AlbC). All were radiolabelled with 99mTc. METHODS: After quality control, each 99mTc marker was incorporated into the albumin of one egg. Then, egg white and yolk were mixed together, and a well-cooked omelette was prepared. Aliquots of the omelette were incubated with an acidic solution of pepsin at 37 degrees C which mimicked gastric juice. Unbound radioactivity in the supernatant fraction was measured at various times up to 3 h. RESULTS: The radiochemical purity was > 95% for all radiopharmaceuticals. During the in-vitro incubation, the percentage of 99mTc labelled colloids released from the omelette increased continuously: after 3 h, 5% for TFC and RSMC, 8% for phytates, and > 9% for the two albumins and RSNC. CONCLUSION: Considering quality controls and release of 99mTc during in-vitro incubation of the omelette, TFC showed the same behaviour as the reference marker RSMC. Thus, TFC seems to be the best candidate to replace RSMC for the radiolabelling of the solid phase of the gastric emptying test meal. SN - 0143-3636 UR - https://www.unboundmedicine.com/medline/citation/16208181/99mTc_ovalbumin_labelled_eggs_for_gastric_emptying_scintigraphy:_in_vitro_comparison_of_solid_food_markers_ DB - PRIME DP - Unbound Medicine ER -