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Characterization of the reconstructed 1918 Spanish influenza pandemic virus.
Science 2005; 310(5745):77-80Sci

Abstract

The pandemic influenza virus of 1918-1919 killed an estimated 20 to 50 million people worldwide. With the recent availability of the complete 1918 influenza virus coding sequence, we used reverse genetics to generate an influenza virus bearing all eight gene segments of the pandemic virus to study the properties associated with its extraordinary virulence. In stark contrast to contemporary human influenza H1N1 viruses, the 1918 pandemic virus had the ability to replicate in the absence of trypsin, caused death in mice and embryonated chicken eggs, and displayed a high-growth phenotype in human bronchial epithelial cells. Moreover, the coordinated expression of the 1918 virus genes most certainly confers the unique high-virulence phenotype observed with this pandemic virus.

Authors+Show Affiliations

Influenza Branch, Mailstop G-16, Division of Viral and Rickettsial Diseases (DVRD), National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, NE, Atlanta, GA 30333, USA. tft9@cdc.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Historical Article
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

16210530

Citation

Tumpey, Terrence M., et al. "Characterization of the Reconstructed 1918 Spanish Influenza Pandemic Virus." Science (New York, N.Y.), vol. 310, no. 5745, 2005, pp. 77-80.
Tumpey TM, Basler CF, Aguilar PV, et al. Characterization of the reconstructed 1918 Spanish influenza pandemic virus. Science. 2005;310(5745):77-80.
Tumpey, T. M., Basler, C. F., Aguilar, P. V., Zeng, H., Solórzano, A., Swayne, D. E., ... García-Sastre, A. (2005). Characterization of the reconstructed 1918 Spanish influenza pandemic virus. Science (New York, N.Y.), 310(5745), pp. 77-80.
Tumpey TM, et al. Characterization of the Reconstructed 1918 Spanish Influenza Pandemic Virus. Science. 2005 Oct 7;310(5745):77-80. PubMed PMID: 16210530.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the reconstructed 1918 Spanish influenza pandemic virus. AU - Tumpey,Terrence M, AU - Basler,Christopher F, AU - Aguilar,Patricia V, AU - Zeng,Hui, AU - Solórzano,Alicia, AU - Swayne,David E, AU - Cox,Nancy J, AU - Katz,Jacqueline M, AU - Taubenberger,Jeffery K, AU - Palese,Peter, AU - García-Sastre,Adolfo, PY - 2005/10/8/pubmed PY - 2005/11/4/medline PY - 2005/10/8/entrez SP - 77 EP - 80 JF - Science (New York, N.Y.) JO - Science VL - 310 IS - 5745 N2 - The pandemic influenza virus of 1918-1919 killed an estimated 20 to 50 million people worldwide. With the recent availability of the complete 1918 influenza virus coding sequence, we used reverse genetics to generate an influenza virus bearing all eight gene segments of the pandemic virus to study the properties associated with its extraordinary virulence. In stark contrast to contemporary human influenza H1N1 viruses, the 1918 pandemic virus had the ability to replicate in the absence of trypsin, caused death in mice and embryonated chicken eggs, and displayed a high-growth phenotype in human bronchial epithelial cells. Moreover, the coordinated expression of the 1918 virus genes most certainly confers the unique high-virulence phenotype observed with this pandemic virus. SN - 1095-9203 UR - https://www.unboundmedicine.com/medline/citation/16210530/Characterization_of_the_reconstructed_1918_Spanish_influenza_pandemic_virus_ L2 - http://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=16210530 DB - PRIME DP - Unbound Medicine ER -