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Titrimetric method for determination of O-desulfated heparin in physiological samples using protamine-sensitive membrane electrode as endpoint detector.
Anal Biochem 2005; 346(2):241-5AB

Abstract

O-Desulfated heparin (ODSH) is a promising new anti-inflammatory agent for the prevention of reperfusion injury following myocardial infarction or stroke. This partially desulfated heparin derivative has less anticoagulant activity than unfractionated heparin but retains the inherent anti-inflammatory properties of heparin. Thus, ODSH could be administered at the high doses needed to achieve desired anti-inflammatory function without risk of hemorrhage. However, given the very low anticoagulant activity of this species, traditional methods for heparin determination in clinical samples might not be well suited for ODSH measurements. In this article, a novel titrimetric method for detection of ODSH in buffer and plasma is described using a protamine-sensitive polymer membrane electrode as the detector. Titrations of ODSH with the heparin antagonist protamine yield sharp endpoints with sensitivity to ODSH in the micrograms per milliliter range for plasma samples. The stoichiometry for protamine interaction with ODSH is determined to average 1.39 microg protamine/microg ODSH in plasma. This technology is further applied to a toxicokinetic study of ODSH in an animal model, demonstrating the ability to detect the changes in ODSH concentrations in biological samples.

Authors+Show Affiliations

Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Validation Studies

Language

eng

PubMed ID

16213460

Citation

Buchanan, Stacey A., et al. "Titrimetric Method for Determination of O-desulfated Heparin in Physiological Samples Using Protamine-sensitive Membrane Electrode as Endpoint Detector." Analytical Biochemistry, vol. 346, no. 2, 2005, pp. 241-5.
Buchanan SA, Kennedy TP, MacArthur RB, et al. Titrimetric method for determination of O-desulfated heparin in physiological samples using protamine-sensitive membrane electrode as endpoint detector. Anal Biochem. 2005;346(2):241-5.
Buchanan, S. A., Kennedy, T. P., MacArthur, R. B., & Meyerhoff, M. E. (2005). Titrimetric method for determination of O-desulfated heparin in physiological samples using protamine-sensitive membrane electrode as endpoint detector. Analytical Biochemistry, 346(2), pp. 241-5.
Buchanan SA, et al. Titrimetric Method for Determination of O-desulfated Heparin in Physiological Samples Using Protamine-sensitive Membrane Electrode as Endpoint Detector. Anal Biochem. 2005 Nov 15;346(2):241-5. PubMed PMID: 16213460.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Titrimetric method for determination of O-desulfated heparin in physiological samples using protamine-sensitive membrane electrode as endpoint detector. AU - Buchanan,Stacey A, AU - Kennedy,Thomas P, AU - MacArthur,Robert B, AU - Meyerhoff,Mark E, Y1 - 2005/09/15/ PY - 2005/06/20/received PY - 2005/08/23/revised PY - 2005/08/30/accepted PY - 2005/10/11/pubmed PY - 2006/3/3/medline PY - 2005/10/11/entrez SP - 241 EP - 5 JF - Analytical biochemistry JO - Anal. Biochem. VL - 346 IS - 2 N2 - O-Desulfated heparin (ODSH) is a promising new anti-inflammatory agent for the prevention of reperfusion injury following myocardial infarction or stroke. This partially desulfated heparin derivative has less anticoagulant activity than unfractionated heparin but retains the inherent anti-inflammatory properties of heparin. Thus, ODSH could be administered at the high doses needed to achieve desired anti-inflammatory function without risk of hemorrhage. However, given the very low anticoagulant activity of this species, traditional methods for heparin determination in clinical samples might not be well suited for ODSH measurements. In this article, a novel titrimetric method for detection of ODSH in buffer and plasma is described using a protamine-sensitive polymer membrane electrode as the detector. Titrations of ODSH with the heparin antagonist protamine yield sharp endpoints with sensitivity to ODSH in the micrograms per milliliter range for plasma samples. The stoichiometry for protamine interaction with ODSH is determined to average 1.39 microg protamine/microg ODSH in plasma. This technology is further applied to a toxicokinetic study of ODSH in an animal model, demonstrating the ability to detect the changes in ODSH concentrations in biological samples. SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/16213460/Titrimetric_method_for_determination_of_O_desulfated_heparin_in_physiological_samples_using_protamine_sensitive_membrane_electrode_as_endpoint_detector_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(05)00645-7 DB - PRIME DP - Unbound Medicine ER -