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Central serotonin depletion affects rat brain areas differently: a qualitative and quantitative comparison between different treatment schemes.
Neurosci Lett. 2006 Jan 09; 392(1-2):129-34.NL

Abstract

Depletion of rat brain serotonin (5-hydroxytryptamin, 5-HT) has been widely used to study effects of serotonin and its interaction with other transmitter systems. Various treatment regimes for serotonin depletion have been applied, but the efficacy of these seems to vary considerably. So far, no studies have systematically examined and compared different approaches. The present work combines quantitative and qualitative measurements and compares six different treatment schemes for 5-HT depletion. Treatment outcome was evaluated by HPLC measurements of 5-HT and 5-HIAA concentrations, and by 5-HT and tyrosine hydroxylase immunocytochemistry. The schemes included repeated administration of fenfluramine (FEN) and/or p-chlorophenylalanine (pCPA). The most efficient treatment for rat brain 5-HT depletion was the combined treatment with one daily pCPA (200 mg/kg) injection for 3 days followed by one injection of d,l-FEN (20 mg/kg) on the fourth day, causing a 94.9% brain 5-HT depletion. Immunostaining revealed a distinct brain distribution of the remaining 5-HT, with an almost complete depletion of 5-HT in the cerebral cortex, hippocampus and thalamus, while a substantial amount of 5-HT still was left in the raphe nuclei, the medial forebrain bundle, and the medial eminence. FEN or pCPA treatment alone caused from 68.2 to 94.0% decrease in 5-HT levels. While the pattern of 5-HT depletion using pCPA alone was comparable to the one seen with the combined treatment, the 5-HT depletion using FEN alone showed a different pattern with 5-HT distributed in several brain regions.

Authors+Show Affiliations

Neurobiology Research Unit, N9201, Copenhagen University Hospital Rigshospitalet, Blegdamsvej 9, Denmark. birgitte@nru.dkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16213658

Citation

Kornum, Birgitte Rahbek, et al. "Central Serotonin Depletion Affects Rat Brain Areas Differently: a Qualitative and Quantitative Comparison Between Different Treatment Schemes." Neuroscience Letters, vol. 392, no. 1-2, 2006, pp. 129-34.
Kornum BR, Licht CL, Weikop P, et al. Central serotonin depletion affects rat brain areas differently: a qualitative and quantitative comparison between different treatment schemes. Neurosci Lett. 2006;392(1-2):129-34.
Kornum, B. R., Licht, C. L., Weikop, P., Knudsen, G. M., & Aznar, S. (2006). Central serotonin depletion affects rat brain areas differently: a qualitative and quantitative comparison between different treatment schemes. Neuroscience Letters, 392(1-2), 129-34.
Kornum BR, et al. Central Serotonin Depletion Affects Rat Brain Areas Differently: a Qualitative and Quantitative Comparison Between Different Treatment Schemes. Neurosci Lett. 2006 Jan 9;392(1-2):129-34. PubMed PMID: 16213658.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Central serotonin depletion affects rat brain areas differently: a qualitative and quantitative comparison between different treatment schemes. AU - Kornum,Birgitte Rahbek, AU - Licht,Cecilie Löe, AU - Weikop,Pia, AU - Knudsen,Gitte Moos, AU - Aznar,Susana, Y1 - 2005/10/06/ PY - 2005/06/07/received PY - 2005/08/22/revised PY - 2005/09/02/accepted PY - 2005/10/11/pubmed PY - 2006/2/24/medline PY - 2005/10/11/entrez SP - 129 EP - 34 JF - Neuroscience letters JO - Neurosci Lett VL - 392 IS - 1-2 N2 - Depletion of rat brain serotonin (5-hydroxytryptamin, 5-HT) has been widely used to study effects of serotonin and its interaction with other transmitter systems. Various treatment regimes for serotonin depletion have been applied, but the efficacy of these seems to vary considerably. So far, no studies have systematically examined and compared different approaches. The present work combines quantitative and qualitative measurements and compares six different treatment schemes for 5-HT depletion. Treatment outcome was evaluated by HPLC measurements of 5-HT and 5-HIAA concentrations, and by 5-HT and tyrosine hydroxylase immunocytochemistry. The schemes included repeated administration of fenfluramine (FEN) and/or p-chlorophenylalanine (pCPA). The most efficient treatment for rat brain 5-HT depletion was the combined treatment with one daily pCPA (200 mg/kg) injection for 3 days followed by one injection of d,l-FEN (20 mg/kg) on the fourth day, causing a 94.9% brain 5-HT depletion. Immunostaining revealed a distinct brain distribution of the remaining 5-HT, with an almost complete depletion of 5-HT in the cerebral cortex, hippocampus and thalamus, while a substantial amount of 5-HT still was left in the raphe nuclei, the medial forebrain bundle, and the medial eminence. FEN or pCPA treatment alone caused from 68.2 to 94.0% decrease in 5-HT levels. While the pattern of 5-HT depletion using pCPA alone was comparable to the one seen with the combined treatment, the 5-HT depletion using FEN alone showed a different pattern with 5-HT distributed in several brain regions. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/16213658/Central_serotonin_depletion_affects_rat_brain_areas_differently:_a_qualitative_and_quantitative_comparison_between_different_treatment_schemes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(05)01047-5 DB - PRIME DP - Unbound Medicine ER -