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Role of oxidative stress in pathophysiology of peripheral neuropathy and modulation by N-acetyl-L-cysteine in rats.
Eur J Pain. 2006 Oct; 10(7):573-9.EJ

Abstract

OBJECTIVES

The objectives of this study were to examine the role of reactive oxygen species and oxidative stress in peripheral neuropathy and behavioural pain responses in experimentally induced chronic constriction injury (CCI) of sciatic nerve of rat. Effect of N-acetyl-L-cysteine (NAC) administered intraperitoneally, was also investigated on CCI-induced neuropathic pain in rats.

METHODS

Neuropathy was induced by CCI of the right sciatic nerve in ketamine anaesthetized rats. Effect of intraperitoneally administered NAC in rats was also investigated using nociceptive behavioural tests. Malondialdehyde, an index of oxidative stress and antioxidant enzymes was also estimated in ligated sciatic nerve.

RESULTS

Behavioural tests, mechanical, thermal and cold stimuli confirmed the development of neuropathic pain after the CCI. The malondialdehyde levels of ligated sciatic nerves were significantly increased compared to non-ligated sciatic nerves (sham operated). The antioxidant enzyme reduced, glutathione was inhibited, while superoxide dismutase increased. However, catalase remained unaffected in the injured sciatic nerves. Intraperitoneal administration of NAC resulted in significant reduction of hyperalgesia in CCI-induced neuropathic rats.

CONCLUSIONS

This study identifies antioxidants superoxide dismutase and reduced glutathione, and oxidative stress as important determinants of neuropathological and behavioural consequences of CCI-induced neuropathy, and NAC may be a potential candidate for alleviation of neuropathic pain.

Authors+Show Affiliations

Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar-243 122 UP, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16214382

Citation

Naik, Ajit K., et al. "Role of Oxidative Stress in Pathophysiology of Peripheral Neuropathy and Modulation By N-acetyl-L-cysteine in Rats." European Journal of Pain (London, England), vol. 10, no. 7, 2006, pp. 573-9.
Naik AK, Tandan SK, Dudhgaonkar SP, et al. Role of oxidative stress in pathophysiology of peripheral neuropathy and modulation by N-acetyl-L-cysteine in rats. Eur J Pain. 2006;10(7):573-9.
Naik, A. K., Tandan, S. K., Dudhgaonkar, S. P., Jadhav, S. H., Kataria, M., Prakash, V. R., & Kumar, D. (2006). Role of oxidative stress in pathophysiology of peripheral neuropathy and modulation by N-acetyl-L-cysteine in rats. European Journal of Pain (London, England), 10(7), 573-9.
Naik AK, et al. Role of Oxidative Stress in Pathophysiology of Peripheral Neuropathy and Modulation By N-acetyl-L-cysteine in Rats. Eur J Pain. 2006;10(7):573-9. PubMed PMID: 16214382.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of oxidative stress in pathophysiology of peripheral neuropathy and modulation by N-acetyl-L-cysteine in rats. AU - Naik,Ajit K, AU - Tandan,Surendra K, AU - Dudhgaonkar,Shailesh P, AU - Jadhav,Sachin H, AU - Kataria,Meena, AU - Prakash,V Ravi, AU - Kumar,Dinesh, Y1 - 2005/10/07/ PY - 2005/03/10/received PY - 2005/08/01/revised PY - 2005/08/03/accepted PY - 2005/10/11/pubmed PY - 2006/12/9/medline PY - 2005/10/11/entrez SP - 573 EP - 9 JF - European journal of pain (London, England) JO - Eur J Pain VL - 10 IS - 7 N2 - OBJECTIVES: The objectives of this study were to examine the role of reactive oxygen species and oxidative stress in peripheral neuropathy and behavioural pain responses in experimentally induced chronic constriction injury (CCI) of sciatic nerve of rat. Effect of N-acetyl-L-cysteine (NAC) administered intraperitoneally, was also investigated on CCI-induced neuropathic pain in rats. METHODS: Neuropathy was induced by CCI of the right sciatic nerve in ketamine anaesthetized rats. Effect of intraperitoneally administered NAC in rats was also investigated using nociceptive behavioural tests. Malondialdehyde, an index of oxidative stress and antioxidant enzymes was also estimated in ligated sciatic nerve. RESULTS: Behavioural tests, mechanical, thermal and cold stimuli confirmed the development of neuropathic pain after the CCI. The malondialdehyde levels of ligated sciatic nerves were significantly increased compared to non-ligated sciatic nerves (sham operated). The antioxidant enzyme reduced, glutathione was inhibited, while superoxide dismutase increased. However, catalase remained unaffected in the injured sciatic nerves. Intraperitoneal administration of NAC resulted in significant reduction of hyperalgesia in CCI-induced neuropathic rats. CONCLUSIONS: This study identifies antioxidants superoxide dismutase and reduced glutathione, and oxidative stress as important determinants of neuropathological and behavioural consequences of CCI-induced neuropathy, and NAC may be a potential candidate for alleviation of neuropathic pain. SN - 1090-3801 UR - https://www.unboundmedicine.com/medline/citation/16214382/Role_of_oxidative_stress_in_pathophysiology_of_peripheral_neuropathy_and_modulation_by_N_acetyl_L_cysteine_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1090-3801(05)00112-6 DB - PRIME DP - Unbound Medicine ER -