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Chloride turnover and ion-transporting activities of yolk-sac preparations (yolk balls) separated from Mozambique tilapia embryos and incubated in freshwater and seawater.
J Exp Biol. 2005 Oct; 208(Pt 20):3851-8.JE

Abstract

We have recently established a unique in vitro experimental model for mitochondrion-rich cell (MRC) research, a ;yolk-ball' incubation system, in which the yolk sac is separated from the embryonic body of Mozambique tilapia embryos and subjected to in vitro incubation. To evaluate the ion-transporting property of the yolk balls, we examined Cl- content and turnover in yolk balls incubated in freshwater and seawater for 48 h, and distribution patterns of three ion transporters, Na+/K+-ATPase, Na+/K+/2Cl- cotransporter (NKCC) and cystic fibrosis transmembrane conductance regulator (CFTR), in MRCs in the yolk-sac membrane. The Cl- turnover rate measured by whole-body influx of 36Cl- was about 60 times higher in yolk balls in seawater than in freshwater, while there was no essential difference in Cl- content between them. Na+/K+-ATPase-immunoreactive MRCs were larger in yolk balls from seawater than yolk balls from freshwater. Distribution patterns of ion-transporting proteins allowed us to classify MRCs in freshwater yolk balls into three types: cells showing only basolateral Na+/K+-ATPase, cells showing basolateral Na+/K+-ATPase and apical NKCC, and cells showing basolateral Na+/K+-ATPase and basolateral NKCC. The seawater yolk balls, on the other hand, were characterized by the appearance of MRCs possessing basolateral Na+/K+-ATPase, basolateral NKCC and apical CFTR. Those seawater-type MRCs were considered to secrete Cl- through the CFTR-positive apical opening to cope with diffusional Cl- influx. These findings indicate that the yolk balls preserve the Cl- transporting property of intact embryos, ensuring the propriety of the yolk ball as an in vitro experimental model for the yolk-sac membrane that contains MRCs.

Authors+Show Affiliations

Department of Anatomy, St Marianna University School of Medicine, Miyamae, Kawasaki 216-8511, Japan. j-hiroi@marianna-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16215213

Citation

Hiroi, Junya, et al. "Chloride Turnover and Ion-transporting Activities of Yolk-sac Preparations (yolk Balls) Separated From Mozambique Tilapia Embryos and Incubated in Freshwater and Seawater." The Journal of Experimental Biology, vol. 208, no. Pt 20, 2005, pp. 3851-8.
Hiroi J, Miyazaki H, Katoh F, et al. Chloride turnover and ion-transporting activities of yolk-sac preparations (yolk balls) separated from Mozambique tilapia embryos and incubated in freshwater and seawater. J Exp Biol. 2005;208(Pt 20):3851-8.
Hiroi, J., Miyazaki, H., Katoh, F., Ohtani-Kaneko, R., & Kaneko, T. (2005). Chloride turnover and ion-transporting activities of yolk-sac preparations (yolk balls) separated from Mozambique tilapia embryos and incubated in freshwater and seawater. The Journal of Experimental Biology, 208(Pt 20), 3851-8.
Hiroi J, et al. Chloride Turnover and Ion-transporting Activities of Yolk-sac Preparations (yolk Balls) Separated From Mozambique Tilapia Embryos and Incubated in Freshwater and Seawater. J Exp Biol. 2005;208(Pt 20):3851-8. PubMed PMID: 16215213.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chloride turnover and ion-transporting activities of yolk-sac preparations (yolk balls) separated from Mozambique tilapia embryos and incubated in freshwater and seawater. AU - Hiroi,Junya, AU - Miyazaki,Hiroaki, AU - Katoh,Fumi, AU - Ohtani-Kaneko,Ritsuko, AU - Kaneko,Toyoji, PY - 2005/10/11/pubmed PY - 2006/1/13/medline PY - 2005/10/11/entrez SP - 3851 EP - 8 JF - The Journal of experimental biology JO - J. Exp. Biol. VL - 208 IS - Pt 20 N2 - We have recently established a unique in vitro experimental model for mitochondrion-rich cell (MRC) research, a ;yolk-ball' incubation system, in which the yolk sac is separated from the embryonic body of Mozambique tilapia embryos and subjected to in vitro incubation. To evaluate the ion-transporting property of the yolk balls, we examined Cl- content and turnover in yolk balls incubated in freshwater and seawater for 48 h, and distribution patterns of three ion transporters, Na+/K+-ATPase, Na+/K+/2Cl- cotransporter (NKCC) and cystic fibrosis transmembrane conductance regulator (CFTR), in MRCs in the yolk-sac membrane. The Cl- turnover rate measured by whole-body influx of 36Cl- was about 60 times higher in yolk balls in seawater than in freshwater, while there was no essential difference in Cl- content between them. Na+/K+-ATPase-immunoreactive MRCs were larger in yolk balls from seawater than yolk balls from freshwater. Distribution patterns of ion-transporting proteins allowed us to classify MRCs in freshwater yolk balls into three types: cells showing only basolateral Na+/K+-ATPase, cells showing basolateral Na+/K+-ATPase and apical NKCC, and cells showing basolateral Na+/K+-ATPase and basolateral NKCC. The seawater yolk balls, on the other hand, were characterized by the appearance of MRCs possessing basolateral Na+/K+-ATPase, basolateral NKCC and apical CFTR. Those seawater-type MRCs were considered to secrete Cl- through the CFTR-positive apical opening to cope with diffusional Cl- influx. These findings indicate that the yolk balls preserve the Cl- transporting property of intact embryos, ensuring the propriety of the yolk ball as an in vitro experimental model for the yolk-sac membrane that contains MRCs. SN - 0022-0949 UR - https://www.unboundmedicine.com/medline/citation/16215213/Chloride_turnover_and_ion_transporting_activities_of_yolk_sac_preparations__yolk_balls__separated_from_Mozambique_tilapia_embryos_and_incubated_in_freshwater_and_seawater_ L2 - http://jeb.biologists.org/cgi/pmidlookup?view=long&pmid=16215213 DB - PRIME DP - Unbound Medicine ER -