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Effect of vitamin E and C supplementation combined with oral antidiabetic therapy on the endothelial dysfunction in the neonatally streptozotocin injected diabetic rat.
Diabetes Metab Res Rev. 2006 May-Jun; 22(3):190-7.DM

Abstract

BACKGROUND

This study investigates the contribution of vitamin supplementation to the efficacy of oral antidiabetic therapy on the reversal of endothelial dysfunction in a model of type-2 diabetes in rat.

METHODS

Diabetes was induced by streptozotocin injection to neonatal rats which were breastfed for 4 weeks, then fed 6 weeks with normal food or food supplemented with 2% vitamin E and 4% vitamin C. Some diabetic rats were treated with gliclazide for 6 weeks. Endothelium-dependent and -independent relaxations to acetylcholine and sodium nitroprusside (SNP) were recorded in thoracic aortic rings. Plasma insulin, HbA(1c) and antioxidant vitamins (A, C and E); plasma and aortic malondialdehyde (MDA) levels were determined.

RESULTS

Induction of diabetes resulted in decreased body weight and increased blood glucose, plasma insulin and HbA(1c) levels compared to controls. Acetylcholine relaxation was impaired in diabetic aorta, while SNP relaxation remained unchanged. Aortic MDA level was significantly higher, while plasma vitamin levels were lower in diabetic rats. Diminished acetylcholine response, enhanced aortic MDA level and decreased plasma vitamin levels were all restored after gliclazide and/or vitamin therapy. However, vitamin supplementation in control rats significantly impaired acetylcholine relaxations and increased aortic MDA levels.

CONCLUSIONS

Apparently, a selective endothelial dysfunction accompanies the imbalance in oxidant/antioxidant status in the type-2 diabetes model of rat and gliclazide and/or vitamin supplementation improves the impairment in diabetic vasculature. However, vitamin supplementation triggers oxidative stress in normal aortic tissue, thereby, leads to endothelial dysfunction; indicating that nutritional extra-supplementation of antioxidant vitamins isn't advisable for normal subjects, although it's beneficial in disease status.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Medicine, Ege University, Izmir, Turkey. gulinnaz.alper@ege.edu.trNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16216038

Citation

Alper, Gülinnaz, et al. "Effect of Vitamin E and C Supplementation Combined With Oral Antidiabetic Therapy On the Endothelial Dysfunction in the Neonatally Streptozotocin Injected Diabetic Rat." Diabetes/metabolism Research and Reviews, vol. 22, no. 3, 2006, pp. 190-7.
Alper G, Olukman M, Irer S, et al. Effect of vitamin E and C supplementation combined with oral antidiabetic therapy on the endothelial dysfunction in the neonatally streptozotocin injected diabetic rat. Diabetes Metab Res Rev. 2006;22(3):190-7.
Alper, G., Olukman, M., Irer, S., Cağlayan, O., Duman, E., Yilmaz, C., & Ulker, S. (2006). Effect of vitamin E and C supplementation combined with oral antidiabetic therapy on the endothelial dysfunction in the neonatally streptozotocin injected diabetic rat. Diabetes/metabolism Research and Reviews, 22(3), 190-7.
Alper G, et al. Effect of Vitamin E and C Supplementation Combined With Oral Antidiabetic Therapy On the Endothelial Dysfunction in the Neonatally Streptozotocin Injected Diabetic Rat. Diabetes Metab Res Rev. 2006 May-Jun;22(3):190-7. PubMed PMID: 16216038.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of vitamin E and C supplementation combined with oral antidiabetic therapy on the endothelial dysfunction in the neonatally streptozotocin injected diabetic rat. AU - Alper,Gülinnaz, AU - Olukman,Murat, AU - Irer,Seda, AU - Cağlayan,Osman, AU - Duman,Erdal, AU - Yilmaz,Candeğer, AU - Ulker,Sibel, PY - 2005/10/11/pubmed PY - 2006/8/17/medline PY - 2005/10/11/entrez SP - 190 EP - 7 JF - Diabetes/metabolism research and reviews JO - Diabetes Metab Res Rev VL - 22 IS - 3 N2 - BACKGROUND: This study investigates the contribution of vitamin supplementation to the efficacy of oral antidiabetic therapy on the reversal of endothelial dysfunction in a model of type-2 diabetes in rat. METHODS: Diabetes was induced by streptozotocin injection to neonatal rats which were breastfed for 4 weeks, then fed 6 weeks with normal food or food supplemented with 2% vitamin E and 4% vitamin C. Some diabetic rats were treated with gliclazide for 6 weeks. Endothelium-dependent and -independent relaxations to acetylcholine and sodium nitroprusside (SNP) were recorded in thoracic aortic rings. Plasma insulin, HbA(1c) and antioxidant vitamins (A, C and E); plasma and aortic malondialdehyde (MDA) levels were determined. RESULTS: Induction of diabetes resulted in decreased body weight and increased blood glucose, plasma insulin and HbA(1c) levels compared to controls. Acetylcholine relaxation was impaired in diabetic aorta, while SNP relaxation remained unchanged. Aortic MDA level was significantly higher, while plasma vitamin levels were lower in diabetic rats. Diminished acetylcholine response, enhanced aortic MDA level and decreased plasma vitamin levels were all restored after gliclazide and/or vitamin therapy. However, vitamin supplementation in control rats significantly impaired acetylcholine relaxations and increased aortic MDA levels. CONCLUSIONS: Apparently, a selective endothelial dysfunction accompanies the imbalance in oxidant/antioxidant status in the type-2 diabetes model of rat and gliclazide and/or vitamin supplementation improves the impairment in diabetic vasculature. However, vitamin supplementation triggers oxidative stress in normal aortic tissue, thereby, leads to endothelial dysfunction; indicating that nutritional extra-supplementation of antioxidant vitamins isn't advisable for normal subjects, although it's beneficial in disease status. SN - 1520-7552 UR - https://www.unboundmedicine.com/medline/citation/16216038/Effect_of_vitamin_E_and_C_supplementation_combined_with_oral_antidiabetic_therapy_on_the_endothelial_dysfunction_in_the_neonatally_streptozotocin_injected_diabetic_rat_ L2 - https://doi.org/10.1002/dmrr.586 DB - PRIME DP - Unbound Medicine ER -