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Detection of DNA damage by alkaline single cell gel electrophoresis in 2,4-dichlorophenoxyacetic-acid- and butachlor-exposed erythrocytes of Clarias batrachus.
Ecotoxicol Environ Saf. 2005 Nov; 62(3):348-54.EE

Abstract

The alkaline single cell gel electrophoresis, also known as comet assay, is a rapid, simple and sensitive technique for measuring DNA strand breaks in individual cells. The present study was undertaken to evaluate the genotoxic potential of two widely used herbicides; 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-chloro-2,6-diethyl-N-(butoxymethyl) acetanilide (butachlor) in erythrocytes of freshwater catfish, Clarias batrachus. Fish were exposed by medium treatment with three sub-lethal concentrations of 2,4-D (25, 50, and 75ppm) and butachlor (1, 2, and 2.5ppm) and alkaline comet assay was performed on nucleated erythrocytes after 48, 72, and 96h. The amount of DNA damage in cells was estimated from comet tail length as the extent of migration of the genetic material. A significant increase in comet tail length indicating DNA damage was observed at all concentrations of both the herbicides compared with control (P<0.05). The mean comet tail length showed a concentration-related and time-dependent increase as the maximum tail length recorded at highest concentration and longer duration of 2,4-D (9.59microm) and butachlor (9.28microm). This study confirmed that the comet assay applied on the fish erythrocyte is a useful tool in determining potential genotoxicity of water pollutants and might be appropriate as a part of a monitoring program.

Authors+Show Affiliations

Gene-Tox Lab, Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh-202002, India. bushra_ateeq@hotmail.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16216628

Citation

Ateeq, Bushra, et al. "Detection of DNA Damage By Alkaline Single Cell Gel Electrophoresis in 2,4-dichlorophenoxyacetic-acid- and Butachlor-exposed Erythrocytes of Clarias Batrachus." Ecotoxicology and Environmental Safety, vol. 62, no. 3, 2005, pp. 348-54.
Ateeq B, Abul Farah M, Ahmad W. Detection of DNA damage by alkaline single cell gel electrophoresis in 2,4-dichlorophenoxyacetic-acid- and butachlor-exposed erythrocytes of Clarias batrachus. Ecotoxicol Environ Saf. 2005;62(3):348-54.
Ateeq, B., Abul Farah, M., & Ahmad, W. (2005). Detection of DNA damage by alkaline single cell gel electrophoresis in 2,4-dichlorophenoxyacetic-acid- and butachlor-exposed erythrocytes of Clarias batrachus. Ecotoxicology and Environmental Safety, 62(3), 348-54.
Ateeq B, Abul Farah M, Ahmad W. Detection of DNA Damage By Alkaline Single Cell Gel Electrophoresis in 2,4-dichlorophenoxyacetic-acid- and Butachlor-exposed Erythrocytes of Clarias Batrachus. Ecotoxicol Environ Saf. 2005;62(3):348-54. PubMed PMID: 16216628.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of DNA damage by alkaline single cell gel electrophoresis in 2,4-dichlorophenoxyacetic-acid- and butachlor-exposed erythrocytes of Clarias batrachus. AU - Ateeq,Bushra, AU - Abul Farah,M, AU - Ahmad,Waseem, PY - 2004/03/06/received PY - 2004/12/13/revised PY - 2004/12/17/accepted PY - 2005/10/12/pubmed PY - 2006/1/7/medline PY - 2005/10/12/entrez SP - 348 EP - 54 JF - Ecotoxicology and environmental safety JO - Ecotoxicol. Environ. Saf. VL - 62 IS - 3 N2 - The alkaline single cell gel electrophoresis, also known as comet assay, is a rapid, simple and sensitive technique for measuring DNA strand breaks in individual cells. The present study was undertaken to evaluate the genotoxic potential of two widely used herbicides; 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-chloro-2,6-diethyl-N-(butoxymethyl) acetanilide (butachlor) in erythrocytes of freshwater catfish, Clarias batrachus. Fish were exposed by medium treatment with three sub-lethal concentrations of 2,4-D (25, 50, and 75ppm) and butachlor (1, 2, and 2.5ppm) and alkaline comet assay was performed on nucleated erythrocytes after 48, 72, and 96h. The amount of DNA damage in cells was estimated from comet tail length as the extent of migration of the genetic material. A significant increase in comet tail length indicating DNA damage was observed at all concentrations of both the herbicides compared with control (P<0.05). The mean comet tail length showed a concentration-related and time-dependent increase as the maximum tail length recorded at highest concentration and longer duration of 2,4-D (9.59microm) and butachlor (9.28microm). This study confirmed that the comet assay applied on the fish erythrocyte is a useful tool in determining potential genotoxicity of water pollutants and might be appropriate as a part of a monitoring program. SN - 0147-6513 UR - https://www.unboundmedicine.com/medline/citation/16216628/Detection_of_DNA_damage_by_alkaline_single_cell_gel_electrophoresis_in_24_dichlorophenoxyacetic_acid__and_butachlor_exposed_erythrocytes_of_Clarias_batrachus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(04)00255-6 DB - PRIME DP - Unbound Medicine ER -