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Cloning of an endangered species (Bos gaurus) using interspecies nuclear transfer.
Cloning. 2000; 2(2):79-90.C

Abstract

Approximately 100 species become extinct a day. Despite increasing interest in using cloning to rescue endangered species, successful interspecies nuclear transfer has not been previously described, and only a few reports of in vitro embryo formation exist. Here we show that interspecies nuclear transfer can be used to clone an endangered species with normal karyotypic and phenotypic development through implantation and the late stages of fetal growth. Somatic cells from a gaur bull (Bos gaurus), a large wild ox on the verge of extinction, (Species Survival Plan < 100 animals) were electrofused with enucleated oocytes from domestic cows. Twelve percent of the reconstructed oocytes developed to the blastocyst stage, and 18% of these embryos developed to the fetal stage when transferred to surrogate mothers. Three of the fetuses were electively removed at days 46 to 54 of gestation, and two continued gestation longer than 180 (ongoing) and 200 days, respectively. Microsatellite marker and cytogenetic analyses confirmed that the nuclear genome of the cloned animals was gaurus in origin. The gaur nuclei were shown to direct normal fetal development, with differentiation into complex tissue and organs, even though the mitochondrial DNA (mtDNA) within all the tissue types evaluated was derived exclusively from the recipient bovine oocytes. These results suggest that somatic cell cloning methods could be used to restore endangered, or even extinct, species and populations.

Authors+Show Affiliations

Advanced Cell Technology, One Innovation Drive, Worcester, MA 01605, USA. rlanza@advancedcell.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

16218862

Citation

Lanza, R P., et al. "Cloning of an Endangered Species (Bos Gaurus) Using Interspecies Nuclear Transfer." Cloning, vol. 2, no. 2, 2000, pp. 79-90.
Lanza RP, Cibelli JB, Diaz F, et al. Cloning of an endangered species (Bos gaurus) using interspecies nuclear transfer. Cloning. 2000;2(2):79-90.
Lanza, R. P., Cibelli, J. B., Diaz, F., Moraes, C. T., Farin, P. W., Farin, C. E., Hammer, C. J., West, M. D., & Damiani, P. (2000). Cloning of an endangered species (Bos gaurus) using interspecies nuclear transfer. Cloning, 2(2), 79-90.
Lanza RP, et al. Cloning of an Endangered Species (Bos Gaurus) Using Interspecies Nuclear Transfer. Cloning. 2000;2(2):79-90. PubMed PMID: 16218862.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning of an endangered species (Bos gaurus) using interspecies nuclear transfer. AU - Lanza,R P, AU - Cibelli,J B, AU - Diaz,F, AU - Moraes,C T, AU - Farin,P W, AU - Farin,C E, AU - Hammer,C J, AU - West,M D, AU - Damiani,P, PY - 2005/10/13/pubmed PY - 2005/12/13/medline PY - 2005/10/13/entrez SP - 79 EP - 90 JF - Cloning JO - Cloning VL - 2 IS - 2 N2 - Approximately 100 species become extinct a day. Despite increasing interest in using cloning to rescue endangered species, successful interspecies nuclear transfer has not been previously described, and only a few reports of in vitro embryo formation exist. Here we show that interspecies nuclear transfer can be used to clone an endangered species with normal karyotypic and phenotypic development through implantation and the late stages of fetal growth. Somatic cells from a gaur bull (Bos gaurus), a large wild ox on the verge of extinction, (Species Survival Plan < 100 animals) were electrofused with enucleated oocytes from domestic cows. Twelve percent of the reconstructed oocytes developed to the blastocyst stage, and 18% of these embryos developed to the fetal stage when transferred to surrogate mothers. Three of the fetuses were electively removed at days 46 to 54 of gestation, and two continued gestation longer than 180 (ongoing) and 200 days, respectively. Microsatellite marker and cytogenetic analyses confirmed that the nuclear genome of the cloned animals was gaurus in origin. The gaur nuclei were shown to direct normal fetal development, with differentiation into complex tissue and organs, even though the mitochondrial DNA (mtDNA) within all the tissue types evaluated was derived exclusively from the recipient bovine oocytes. These results suggest that somatic cell cloning methods could be used to restore endangered, or even extinct, species and populations. SN - 1520-4553 UR - https://www.unboundmedicine.com/medline/citation/16218862/Cloning_of_an_endangered_species__Bos_gaurus__using_interspecies_nuclear_transfer_ L2 - https://medlineplus.gov/cloning.html DB - PRIME DP - Unbound Medicine ER -