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Effect of iron on lipid peroxidation, and enzymatic and non-enzymatic antioxidants and bacoside-A content in medicinal plant Bacopa monnieri L.
Chemosphere. 2006 Mar; 62(8):1340-50.C

Abstract

The effect of Fe was investigated in medicinally important plant, Bacopa monnieri L. and the response on malondialdehyde (MDA) content, superoxide dismutase (SOD), peroxidase (POD) and ascorbate peroxidase (APX) was found different in roots and leaves of the metal treated plants. Iron induced stress was observed as indicated by high level of lipid peroxidation, being more steep increase in leaves than roots. In roots, SOD activity was found to increase in metal treated plants except 80 and 160 microM at 72 h, whereas, it decreased in leaves except 10 and 40 microM after 48 h as compared to their respective controls. Among H2O2 eliminating enzymes, POD activity increased in roots, however, it decreased in leaves except at 10 and 40 microM Fe after 48 h as compared to control. At 24 and 48 h, APX activity and ascorbic acid content followed the similar trend and were found to increase in both parts of the metal treated plants as compared to their respective controls. The level of cysteine content in the roots increased at initial period of exposure; however, no marked change in its content was noticed in leaves. In both roots and leaves, non-protein thiol content was found to increase except at higher metal concentrations at 72 h. The data of proline content have shown significant (p<0.01) increase at 40 microM onwards in both part of the plants after 48 and 72 h. Correlation coefficient was evaluated between metal accumulations with various parameters and also between different antioxidant parameters with MDA. Since the level of bacoside-A (active constituent) content in metal treated plants increases, therefore, it is advisable to assess the biological activity of the plants before using for medicinal purposes, particularly in developing countries.

Authors+Show Affiliations

Ecotoxicology and Bioremediation Group, National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, India. sinha_sarita@rediffmail.comNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16219336

Citation

Sinha, Sarita, and Rohit Saxena. "Effect of Iron On Lipid Peroxidation, and Enzymatic and Non-enzymatic Antioxidants and bacoside-A Content in Medicinal Plant Bacopa Monnieri L." Chemosphere, vol. 62, no. 8, 2006, pp. 1340-50.
Sinha S, Saxena R. Effect of iron on lipid peroxidation, and enzymatic and non-enzymatic antioxidants and bacoside-A content in medicinal plant Bacopa monnieri L. Chemosphere. 2006;62(8):1340-50.
Sinha, S., & Saxena, R. (2006). Effect of iron on lipid peroxidation, and enzymatic and non-enzymatic antioxidants and bacoside-A content in medicinal plant Bacopa monnieri L. Chemosphere, 62(8), 1340-50.
Sinha S, Saxena R. Effect of Iron On Lipid Peroxidation, and Enzymatic and Non-enzymatic Antioxidants and bacoside-A Content in Medicinal Plant Bacopa Monnieri L. Chemosphere. 2006;62(8):1340-50. PubMed PMID: 16219336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of iron on lipid peroxidation, and enzymatic and non-enzymatic antioxidants and bacoside-A content in medicinal plant Bacopa monnieri L. AU - Sinha,Sarita, AU - Saxena,Rohit, Y1 - 2005/10/10/ PY - 2004/12/14/received PY - 2005/06/14/revised PY - 2005/07/01/accepted PY - 2005/10/13/pubmed PY - 2006/4/28/medline PY - 2005/10/13/entrez SP - 1340 EP - 50 JF - Chemosphere JO - Chemosphere VL - 62 IS - 8 N2 - The effect of Fe was investigated in medicinally important plant, Bacopa monnieri L. and the response on malondialdehyde (MDA) content, superoxide dismutase (SOD), peroxidase (POD) and ascorbate peroxidase (APX) was found different in roots and leaves of the metal treated plants. Iron induced stress was observed as indicated by high level of lipid peroxidation, being more steep increase in leaves than roots. In roots, SOD activity was found to increase in metal treated plants except 80 and 160 microM at 72 h, whereas, it decreased in leaves except 10 and 40 microM after 48 h as compared to their respective controls. Among H2O2 eliminating enzymes, POD activity increased in roots, however, it decreased in leaves except at 10 and 40 microM Fe after 48 h as compared to control. At 24 and 48 h, APX activity and ascorbic acid content followed the similar trend and were found to increase in both parts of the metal treated plants as compared to their respective controls. The level of cysteine content in the roots increased at initial period of exposure; however, no marked change in its content was noticed in leaves. In both roots and leaves, non-protein thiol content was found to increase except at higher metal concentrations at 72 h. The data of proline content have shown significant (p<0.01) increase at 40 microM onwards in both part of the plants after 48 and 72 h. Correlation coefficient was evaluated between metal accumulations with various parameters and also between different antioxidant parameters with MDA. Since the level of bacoside-A (active constituent) content in metal treated plants increases, therefore, it is advisable to assess the biological activity of the plants before using for medicinal purposes, particularly in developing countries. SN - 0045-6535 UR - https://www.unboundmedicine.com/medline/citation/16219336/Effect_of_iron_on_lipid_peroxidation_and_enzymatic_and_non_enzymatic_antioxidants_and_bacoside_A_content_in_medicinal_plant_Bacopa_monnieri_L_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0045-6535(05)00940-9 DB - PRIME DP - Unbound Medicine ER -