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Assessment of the phototoxic hazard of some essential oils using modified 3T3 neutral red uptake assay.
Toxicol In Vitro 2006; 20(4):480-9TV

Abstract

When substances are developed in the aim to be a constituent of personal care products, and to be applied on the skin, it is necessary to carry out an assessment of potential phototoxic hazard. Phototoxicity is skin reaction caused by concurrent topical or systemic exposure to specific molecule and ultraviolet radiation. Most phototoxic compounds absorb energy particularly from UVA light leading to the generation of activated derivatives which can induce cellular damage. This type of adverse cutaneous response can be reproduced in vitro using different models of phototoxicity such as the validated 3T3 Neutral Red Uptake (NRU) phototoxicity assay. In the present study we utilised two different cell lines (the murine fibroblastic cell line 3T3 and the rabbit cornea derived cell line SIRC) to compare the photo-irritation potential of a strong phototoxic compound, chlorpromazine, to a weaker composite, such as 8-methoxypsoralen and Bergamot oil. After comparison of the different systems, five other essential oils were tested with both cell lines. Cellular damage was evaluated by the NRU cytotoxicity test or by MTT conversion test.

Authors+Show Affiliations

Yves-Rocher Research and Development, Worldwide Safety Department, 101 quai du Président Roosevelt, 92444 Issy les Moulineaux, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16219446

Citation

Dijoux, Nathalie, et al. "Assessment of the Phototoxic Hazard of some Essential Oils Using Modified 3T3 Neutral Red Uptake Assay." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 20, no. 4, 2006, pp. 480-9.
Dijoux N, Guingand Y, Bourgeois C, et al. Assessment of the phototoxic hazard of some essential oils using modified 3T3 neutral red uptake assay. Toxicol In Vitro. 2006;20(4):480-9.
Dijoux, N., Guingand, Y., Bourgeois, C., Durand, S., Fromageot, C., Combe, C., & Ferret, P. J. (2006). Assessment of the phototoxic hazard of some essential oils using modified 3T3 neutral red uptake assay. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 20(4), pp. 480-9.
Dijoux N, et al. Assessment of the Phototoxic Hazard of some Essential Oils Using Modified 3T3 Neutral Red Uptake Assay. Toxicol In Vitro. 2006;20(4):480-9. PubMed PMID: 16219446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of the phototoxic hazard of some essential oils using modified 3T3 neutral red uptake assay. AU - Dijoux,Nathalie, AU - Guingand,Yannick, AU - Bourgeois,Caroline, AU - Durand,Sandrine, AU - Fromageot,Claude, AU - Combe,Corinne, AU - Ferret,Pierre-Jacques, Y1 - 2005/10/10/ PY - 2005/03/04/received PY - 2005/08/04/revised PY - 2005/08/29/accepted PY - 2005/10/13/pubmed PY - 2006/7/26/medline PY - 2005/10/13/entrez SP - 480 EP - 9 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 20 IS - 4 N2 - When substances are developed in the aim to be a constituent of personal care products, and to be applied on the skin, it is necessary to carry out an assessment of potential phototoxic hazard. Phototoxicity is skin reaction caused by concurrent topical or systemic exposure to specific molecule and ultraviolet radiation. Most phototoxic compounds absorb energy particularly from UVA light leading to the generation of activated derivatives which can induce cellular damage. This type of adverse cutaneous response can be reproduced in vitro using different models of phototoxicity such as the validated 3T3 Neutral Red Uptake (NRU) phototoxicity assay. In the present study we utilised two different cell lines (the murine fibroblastic cell line 3T3 and the rabbit cornea derived cell line SIRC) to compare the photo-irritation potential of a strong phototoxic compound, chlorpromazine, to a weaker composite, such as 8-methoxypsoralen and Bergamot oil. After comparison of the different systems, five other essential oils were tested with both cell lines. Cellular damage was evaluated by the NRU cytotoxicity test or by MTT conversion test. SN - 0887-2333 UR - https://www.unboundmedicine.com/medline/citation/16219446/Assessment_of_the_phototoxic_hazard_of_some_essential_oils_using_modified_3T3_neutral_red_uptake_assay L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(05)00174-8 DB - PRIME DP - Unbound Medicine ER -