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The essential WD-repeat protein Rsa4p is required for rRNA processing and intra-nuclear transport of 60S ribosomal subunits.
Nucleic Acids Res. 2005; 33(18):5728-39.NA

Abstract

We report the characterization of a novel factor, Rsa4p (Ycr072cp), which is essential for the synthesis of 60S ribosomal subunits. Rsa4p is a conserved WD-repeat protein that seems to localize in the nucleolus. In vivo depletion of Rsa4p results in a deficit of 60S ribosomal subunits and the appearance of half-mer polysomes. Northern hybridization and primer extension analyses of pre-rRNA and mature rRNAs show that depletion of Rsa4p leads to the accumulation of the 27S, 25.5S and 7S pre-rRNAs, resulting in a reduction of the mature 25S and 5.8S rRNAs. Pulse-chase analyses of pre-rRNA processing reveal that, at least, this is due to a strong delay in the maturation of 27S pre-rRNA intermediates to mature 25S rRNA. Furthermore, depletion of Rsa4p inhibited the release of the pre-60S ribosomal particles from the nucleolus to the nucleoplasm, as judged by the predominantly nucleolar accumulation of the large subunit Rpl25-eGFP reporter construct. We propose that Rsa4p associates early with pre-60S ribosomal particles and provides a platform of interaction for correct processing of rRNA precursors and nucleolar release of 60S ribosomal subunits.

Authors+Show Affiliations

Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Avda Reina Mercedes, 6 Apartado 1095, E-41012 Sevilla, Spain. jdlcd@us.esNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16221974

Citation

de la Cruz, Jesús, et al. "The Essential WD-repeat Protein Rsa4p Is Required for rRNA Processing and Intra-nuclear Transport of 60S Ribosomal Subunits." Nucleic Acids Research, vol. 33, no. 18, 2005, pp. 5728-39.
de la Cruz J, Sanz-Martínez E, Remacha M. The essential WD-repeat protein Rsa4p is required for rRNA processing and intra-nuclear transport of 60S ribosomal subunits. Nucleic Acids Res. 2005;33(18):5728-39.
de la Cruz, J., Sanz-Martínez, E., & Remacha, M. (2005). The essential WD-repeat protein Rsa4p is required for rRNA processing and intra-nuclear transport of 60S ribosomal subunits. Nucleic Acids Research, 33(18), 5728-39.
de la Cruz J, Sanz-Martínez E, Remacha M. The Essential WD-repeat Protein Rsa4p Is Required for rRNA Processing and Intra-nuclear Transport of 60S Ribosomal Subunits. Nucleic Acids Res. 2005;33(18):5728-39. PubMed PMID: 16221974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The essential WD-repeat protein Rsa4p is required for rRNA processing and intra-nuclear transport of 60S ribosomal subunits. AU - de la Cruz,Jesús, AU - Sanz-Martínez,Eloisa, AU - Remacha,Miguel, Y1 - 2005/10/12/ PY - 2005/10/14/pubmed PY - 2005/10/26/medline PY - 2005/10/14/entrez SP - 5728 EP - 39 JF - Nucleic acids research JO - Nucleic Acids Res. VL - 33 IS - 18 N2 - We report the characterization of a novel factor, Rsa4p (Ycr072cp), which is essential for the synthesis of 60S ribosomal subunits. Rsa4p is a conserved WD-repeat protein that seems to localize in the nucleolus. In vivo depletion of Rsa4p results in a deficit of 60S ribosomal subunits and the appearance of half-mer polysomes. Northern hybridization and primer extension analyses of pre-rRNA and mature rRNAs show that depletion of Rsa4p leads to the accumulation of the 27S, 25.5S and 7S pre-rRNAs, resulting in a reduction of the mature 25S and 5.8S rRNAs. Pulse-chase analyses of pre-rRNA processing reveal that, at least, this is due to a strong delay in the maturation of 27S pre-rRNA intermediates to mature 25S rRNA. Furthermore, depletion of Rsa4p inhibited the release of the pre-60S ribosomal particles from the nucleolus to the nucleoplasm, as judged by the predominantly nucleolar accumulation of the large subunit Rpl25-eGFP reporter construct. We propose that Rsa4p associates early with pre-60S ribosomal particles and provides a platform of interaction for correct processing of rRNA precursors and nucleolar release of 60S ribosomal subunits. SN - 1362-4962 UR - https://www.unboundmedicine.com/medline/citation/16221974/The_essential_WD_repeat_protein_Rsa4p_is_required_for_rRNA_processing_and_intra_nuclear_transport_of_60S_ribosomal_subunits_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gki887 DB - PRIME DP - Unbound Medicine ER -