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Oxidative stress in Graves' disease patients and antioxidant protection against lymphocytes DNA damage in vitro.
Pharmazie. 2005 Sep; 60(9):696-700.P

Abstract

DNA damage to peripheral blood lymphocytes of patients with Graves' disease (GD) was studied in vitro before and after treatment with antioxidants, melatonin, quercetin, N-acetylcysteine (NAC) and vitamin C. DNA damage (comet %) was remarkably higher in patients (23.7 +/- 5.5%) than that in healthy persons (9.8 +/- 3.2%, p < 0.01). Plasma malondialdehyde (MDA) content (7.90 +/- 1.77 microM) of patients was significantly higher than that of healthy persons (4.71 +/- 1.19 microM, p < 0.01). Also, the plasma total antioxidant capacity (TAC) (7.53 +/- 1.35 U/ml) in GD patients was significantly lower than that in healthy persons (10.56 +/- 2.21 U/ml, p < 0.01). Negative correlations were observed between plasma TAC and DNA damage in lymphocytes (r = -0.599, p < 0.01), and between plasma TAC and MDA (r = -0.40, p < 0.05) in GD patients. After treatment with 100 microM melatonin, quercetin or NAC for 4 h in vitro, DNA damage in lymphocytes in GD patients declined significantly (from 23.8 +/- 4.4% to 14.4 +/- 4.0%, p < 0.001 for melatonin, from 23.4 +/- 4.7% to 18.1 +/- 4.3%, p < 0.01 for quercetin, from 23.7 +/- 4.0% to 18.7 +/- 5.7%, p < 0.05 for NAC), while there was little change with concentrations of 1-100 microM of vitamin C. However, 1000 microM vitamin C enhanced DNA damage significantly (from 23.8 +/- 2.3% to 30.3 +/- 3.9%, p < 0.05). Our results showed that oxidative stress existed in GD patients and the antioxidants melatonin, quercetin and NAC are beneficial for DNA damage in lymphocytes of GD patients in vitro.

Authors+Show Affiliations

Tang XL
School of Life Sciences, Lanzhou University, China.
Liu XJ
No affiliation info available
Sun WM
No affiliation info available
Zhao J
No affiliation info available
Zheng RL
No affiliation info available

MeSH

AcetylcysteineAdultAntioxidantsAscorbic AcidComet AssayDNA DamageFemaleGraves DiseaseHumansIndicators and ReagentsLymphocytesMaleMalondialdehydeMelatoninOxidative StressQuercetinThyroid GlandThyrotropinThyroxineTriiodothyronine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16222871

Citation

Tang, Xu-Lei, et al. "Oxidative Stress in Graves' Disease Patients and Antioxidant Protection Against Lymphocytes DNA Damage in Vitro." Die Pharmazie, vol. 60, no. 9, 2005, pp. 696-700.
Tang XL, Liu XJ, Sun WM, et al. Oxidative stress in Graves' disease patients and antioxidant protection against lymphocytes DNA damage in vitro. Pharmazie. 2005;60(9):696-700.
Tang, X. L., Liu, X. J., Sun, W. M., Zhao, J., & Zheng, R. L. (2005). Oxidative stress in Graves' disease patients and antioxidant protection against lymphocytes DNA damage in vitro. Die Pharmazie, 60(9), 696-700.
Tang XL, et al. Oxidative Stress in Graves' Disease Patients and Antioxidant Protection Against Lymphocytes DNA Damage in Vitro. Pharmazie. 2005;60(9):696-700. PubMed PMID: 16222871.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidative stress in Graves' disease patients and antioxidant protection against lymphocytes DNA damage in vitro. AU - Tang,Xu-Lei, AU - Liu,Xiao-Ju, AU - Sun,Wei-Ming, AU - Zhao,Jin, AU - Zheng,Rong-Liang, PY - 2005/10/15/pubmed PY - 2005/12/13/medline PY - 2005/10/15/entrez SP - 696 EP - 700 JF - Die Pharmazie JO - Pharmazie VL - 60 IS - 9 N2 - DNA damage to peripheral blood lymphocytes of patients with Graves' disease (GD) was studied in vitro before and after treatment with antioxidants, melatonin, quercetin, N-acetylcysteine (NAC) and vitamin C. DNA damage (comet %) was remarkably higher in patients (23.7 +/- 5.5%) than that in healthy persons (9.8 +/- 3.2%, p < 0.01). Plasma malondialdehyde (MDA) content (7.90 +/- 1.77 microM) of patients was significantly higher than that of healthy persons (4.71 +/- 1.19 microM, p < 0.01). Also, the plasma total antioxidant capacity (TAC) (7.53 +/- 1.35 U/ml) in GD patients was significantly lower than that in healthy persons (10.56 +/- 2.21 U/ml, p < 0.01). Negative correlations were observed between plasma TAC and DNA damage in lymphocytes (r = -0.599, p < 0.01), and between plasma TAC and MDA (r = -0.40, p < 0.05) in GD patients. After treatment with 100 microM melatonin, quercetin or NAC for 4 h in vitro, DNA damage in lymphocytes in GD patients declined significantly (from 23.8 +/- 4.4% to 14.4 +/- 4.0%, p < 0.001 for melatonin, from 23.4 +/- 4.7% to 18.1 +/- 4.3%, p < 0.01 for quercetin, from 23.7 +/- 4.0% to 18.7 +/- 5.7%, p < 0.05 for NAC), while there was little change with concentrations of 1-100 microM of vitamin C. However, 1000 microM vitamin C enhanced DNA damage significantly (from 23.8 +/- 2.3% to 30.3 +/- 3.9%, p < 0.05). Our results showed that oxidative stress existed in GD patients and the antioxidants melatonin, quercetin and NAC are beneficial for DNA damage in lymphocytes of GD patients in vitro. SN - 0031-7144 UR - https://www.unboundmedicine.com/medline/citation/16222871/Oxidative_stress_in_Graves'_disease_patients_and_antioxidant_protection_against_lymphocytes_DNA_damage_in_vitro_ DB - PRIME DP - Unbound Medicine ER -