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Polyclonal emergence and importation of community-acquired methicillin-resistant Staphylococcus aureus strains harbouring Panton-Valentine leucocidin genes in Belgium.
J Antimicrob Chemother 2005; 56(6):1103-6JA

Abstract

OBJECTIVES

Worldwide spread of a limited number of Panton-Valentine leucocidin (PVL) -producing methicillin-resistant Staphylococcus aureus (MRSA) clones has been reported in various communities. The objective of this study was to describe the molecular characteristics of the first PVL-positive MRSA strains isolated in Belgium.

METHODS

Clinical MRSA isolates (n = 41) collected from 2002 to 2004 from Belgian patients were investigated for the PVL gene by PCR. PVL-positive isolates were genotyped by PFGE, staphylococcal cassette chromosome mec (SCCmec) typing, spa sequence typing, accessory gene regulator (agr) polymorphism and multi-locus sequence typing (MLST). Susceptibility to 14 antimicrobials was determined by the disc diffusion method. Genes encoding resistance to tetracyclines, aminoglycosides and macrolide-lincosamide-streptogramin were determined by PCR.

RESULTS

Sixteen isolates carried lukS-lukF genes that encode the PVL toxin. All but one isolate were community-acquired. Three patients reported recent travel to North Africa and South America. They were associated with skin or soft tissue infections, bacteraemia and peritonitis. By molecular typing, they belonged to five genotypes: ST80-SCCmec IV, ST8-SCCmec IV, ST30-SCCmec IV, ST153-SCCmec IV and ST88-SCCmec IV. They belonged to the agr type 3 except for ST8 strains, which showed agr type 1. All isolates were susceptible to fluoroquinolones. Approximately, half of them were resistant to tetracycline, fusidic acid and kanamycin. Tetracycline-resistant strains harboured the tet(K) gene and resistance to kanamycin was associated with the aph3'-IIIa gene. The single erythromycin-resistant isolate harboured msr(A/B) genes conferring the M resistance phenotype.

CONCLUSIONS

These results indicate the recent emergence and sporadic importation into Belgium of PVL-positive community-associated MRSA strains belonging to five distinct clones.

Authors+Show Affiliations

Laboratoire de Référence MRSA--Staphylocoques, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium. odenis@ulb.ac.beNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16223937

Citation

Denis, O, et al. "Polyclonal Emergence and Importation of Community-acquired Methicillin-resistant Staphylococcus Aureus Strains Harbouring Panton-Valentine Leucocidin Genes in Belgium." The Journal of Antimicrobial Chemotherapy, vol. 56, no. 6, 2005, pp. 1103-6.
Denis O, Deplano A, De Beenhouwer H, et al. Polyclonal emergence and importation of community-acquired methicillin-resistant Staphylococcus aureus strains harbouring Panton-Valentine leucocidin genes in Belgium. J Antimicrob Chemother. 2005;56(6):1103-6.
Denis, O., Deplano, A., De Beenhouwer, H., Hallin, M., Huysmans, G., Garrino, M. G., ... Struelens, M. J. (2005). Polyclonal emergence and importation of community-acquired methicillin-resistant Staphylococcus aureus strains harbouring Panton-Valentine leucocidin genes in Belgium. The Journal of Antimicrobial Chemotherapy, 56(6), pp. 1103-6.
Denis O, et al. Polyclonal Emergence and Importation of Community-acquired Methicillin-resistant Staphylococcus Aureus Strains Harbouring Panton-Valentine Leucocidin Genes in Belgium. J Antimicrob Chemother. 2005;56(6):1103-6. PubMed PMID: 16223937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyclonal emergence and importation of community-acquired methicillin-resistant Staphylococcus aureus strains harbouring Panton-Valentine leucocidin genes in Belgium. AU - Denis,O, AU - Deplano,A, AU - De Beenhouwer,H, AU - Hallin,M, AU - Huysmans,G, AU - Garrino,M G, AU - Glupczynski,Y, AU - Malaviolle,X, AU - Vergison,A, AU - Struelens,M J, Y1 - 2005/10/13/ PY - 2005/10/15/pubmed PY - 2006/3/22/medline PY - 2005/10/15/entrez SP - 1103 EP - 6 JF - The Journal of antimicrobial chemotherapy JO - J. Antimicrob. Chemother. VL - 56 IS - 6 N2 - OBJECTIVES: Worldwide spread of a limited number of Panton-Valentine leucocidin (PVL) -producing methicillin-resistant Staphylococcus aureus (MRSA) clones has been reported in various communities. The objective of this study was to describe the molecular characteristics of the first PVL-positive MRSA strains isolated in Belgium. METHODS: Clinical MRSA isolates (n = 41) collected from 2002 to 2004 from Belgian patients were investigated for the PVL gene by PCR. PVL-positive isolates were genotyped by PFGE, staphylococcal cassette chromosome mec (SCCmec) typing, spa sequence typing, accessory gene regulator (agr) polymorphism and multi-locus sequence typing (MLST). Susceptibility to 14 antimicrobials was determined by the disc diffusion method. Genes encoding resistance to tetracyclines, aminoglycosides and macrolide-lincosamide-streptogramin were determined by PCR. RESULTS: Sixteen isolates carried lukS-lukF genes that encode the PVL toxin. All but one isolate were community-acquired. Three patients reported recent travel to North Africa and South America. They were associated with skin or soft tissue infections, bacteraemia and peritonitis. By molecular typing, they belonged to five genotypes: ST80-SCCmec IV, ST8-SCCmec IV, ST30-SCCmec IV, ST153-SCCmec IV and ST88-SCCmec IV. They belonged to the agr type 3 except for ST8 strains, which showed agr type 1. All isolates were susceptible to fluoroquinolones. Approximately, half of them were resistant to tetracycline, fusidic acid and kanamycin. Tetracycline-resistant strains harboured the tet(K) gene and resistance to kanamycin was associated with the aph3'-IIIa gene. The single erythromycin-resistant isolate harboured msr(A/B) genes conferring the M resistance phenotype. CONCLUSIONS: These results indicate the recent emergence and sporadic importation into Belgium of PVL-positive community-associated MRSA strains belonging to five distinct clones. SN - 0305-7453 UR - https://www.unboundmedicine.com/medline/citation/16223937/Polyclonal_emergence_and_importation_of_community_acquired_methicillin_resistant_Staphylococcus_aureus_strains_harbouring_Panton_Valentine_leucocidin_genes_in_Belgium_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dki379 DB - PRIME DP - Unbound Medicine ER -