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[Expression of SARS-CoV in various types of cells in lung tissues].
Beijing Da Xue Xue Bao Yi Xue Ban. 2005 Oct 18; 37(5):453-7.BD

Abstract

OBJECTIVE

To investigate the cell types infected by severe acute respiratory syndrome-associated coronavirus (SARS-CoV) in lung tissues and explore the mechanism of lung injury in SARS.

METHODS

In-situ hybridization(ISH) and immunohistochemistry(IHC) double staining was applied to study the lung tissues from 7 SARS cases of Beijing and one of Anhui province. According to SARS-CoV genome sequence, the cDNA probe was synthesized and labelled by digoxin. Immunohistochemically, antibodies of cytokeratin(CK), CD34, CD68, Vimentin and CD3 were applied to demonstrate bronchial epithelial cells, type II pneumocytes, endothelial cells, macrophages, fibroblasts and T cells respectively.

RESULTS

The positive results of in-situ hybridization showed that the lung tissues of all cases expressed SARS-CoV RNA, and positive signals displayed in cytoplasms (purple-blue, NBP-BCIP. ISH-IHC double staining showed that positive signals of both ISH (purple-blue NBT-BCIP and IHC (red-brown, AEC expressed in the cytoplasms (purple and red). The positive results of double staining indicated that bronchial epithelial cells, type II pneumocytes, endothelial cells, macrophages, fibroblasts and T lymphocytes were diffusely infected by SARS-CoV.

CONCLUSION

This study of ISH-IHC double staining in lung tissues of SARS patients showed that bronchial epithelial cells, type II pneumocytes, endothelial cells, macrophages, T lymphocytes and fibroblasts were attacked diffusely in SARS lungs. Various types of cells damaged by SARS-CoV and inflammatory mediators released by those cells play an important role in the pathogenesis of lung injury in SARS.

Authors+Show Affiliations

Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100083, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16224511

Citation

Lu, Yuan, et al. "[Expression of SARS-CoV in Various Types of Cells in Lung Tissues]." Beijing Da Xue Xue Bao. Yi Xue Ban = Journal of Peking University. Health Sciences, vol. 37, no. 5, 2005, pp. 453-7.
Lu Y, Gong EC, Zhang QY, et al. [Expression of SARS-CoV in various types of cells in lung tissues]. Beijing Da Xue Xue Bao Yi Xue Ban. 2005;37(5):453-7.
Lu, Y., Gong, E. C., Zhang, Q. Y., Gu, J., Li, X. W., Zhang, B., Hou, L., Shao, H. Q., Gao, Z. F., Zheng, J., Fang, W. G., & Zhong, Y. F. (2005). [Expression of SARS-CoV in various types of cells in lung tissues]. Beijing Da Xue Xue Bao. Yi Xue Ban = Journal of Peking University. Health Sciences, 37(5), 453-7.
Lu Y, et al. [Expression of SARS-CoV in Various Types of Cells in Lung Tissues]. Beijing Da Xue Xue Bao Yi Xue Ban. 2005 Oct 18;37(5):453-7. PubMed PMID: 16224511.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression of SARS-CoV in various types of cells in lung tissues]. AU - Lu,Yuan, AU - Gong,En-cong, AU - Zhang,Qian-ying, AU - Gu,Jiang, AU - Li,Xing-wang, AU - Zhang,Bo, AU - Hou,Lin, AU - Shao,Hong-quan, AU - Gao,Zi-fen, AU - Zheng,Jie, AU - Fang,Wei-gang, AU - Zhong,Yan-feng, PY - 2005/10/15/pubmed PY - 2008/6/24/medline PY - 2005/10/15/entrez SP - 453 EP - 7 JF - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences JO - Beijing Da Xue Xue Bao Yi Xue Ban VL - 37 IS - 5 N2 - OBJECTIVE: To investigate the cell types infected by severe acute respiratory syndrome-associated coronavirus (SARS-CoV) in lung tissues and explore the mechanism of lung injury in SARS. METHODS: In-situ hybridization(ISH) and immunohistochemistry(IHC) double staining was applied to study the lung tissues from 7 SARS cases of Beijing and one of Anhui province. According to SARS-CoV genome sequence, the cDNA probe was synthesized and labelled by digoxin. Immunohistochemically, antibodies of cytokeratin(CK), CD34, CD68, Vimentin and CD3 were applied to demonstrate bronchial epithelial cells, type II pneumocytes, endothelial cells, macrophages, fibroblasts and T cells respectively. RESULTS: The positive results of in-situ hybridization showed that the lung tissues of all cases expressed SARS-CoV RNA, and positive signals displayed in cytoplasms (purple-blue, NBP-BCIP. ISH-IHC double staining showed that positive signals of both ISH (purple-blue NBT-BCIP and IHC (red-brown, AEC expressed in the cytoplasms (purple and red). The positive results of double staining indicated that bronchial epithelial cells, type II pneumocytes, endothelial cells, macrophages, fibroblasts and T lymphocytes were diffusely infected by SARS-CoV. CONCLUSION: This study of ISH-IHC double staining in lung tissues of SARS patients showed that bronchial epithelial cells, type II pneumocytes, endothelial cells, macrophages, T lymphocytes and fibroblasts were attacked diffusely in SARS lungs. Various types of cells damaged by SARS-CoV and inflammatory mediators released by those cells play an important role in the pathogenesis of lung injury in SARS. SN - 1671-167X UR - https://www.unboundmedicine.com/medline/citation/16224511/[Expression_of_SARS_CoV_in_various_types_of_cells_in_lung_tissues]_ L2 - http://xuebao.bjmu.edu.cn/EN/Y2005/V37/I5/453 DB - PRIME DP - Unbound Medicine ER -