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Involvement of tumor necrosis factor-related apoptosis-inducing ligand and tumor necrosis factor-related apoptosis-inducing ligand receptors in viral hepatic diseases.
Hum Pathol. 2005 Oct; 36(10):1066-73.HP

Abstract

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces apoptosis in tumor cells, but not in most normal cells. The role of TRAIL in hepatic cell death and hepatic diseases is not well understood. The present study investigated the expression of TRAIL and TRAIL receptors (TRAIL-Rs) in patients with hepatitis C virus infection using immunohistochemistry and examined physiological roles under viral infection in the HepG2 cell line. Staining of TRAIL or TRAIL-Rs was prominent in the cytoplasm and membrane of hepatocytes in the periportal area. Some liver-infiltrating lymphocytes also displayed positive staining for TRAIL. Staining intensity was significantly increased with disease progression, particularly in the periportal area. AdCMVLacZ (Q-BIOgene, Carisbad, Calif) infection was also found to induce apoptosis in HepG2 cells and significantly augment TRAIL-induced apoptosis. Anti-TRAIL antibody significantly inhibited apoptosis induced by AdCMVLacZ infection. Flow cytometry analysis revealed that both TRAIL-R2 and TRAIL were up-regulated on the cell surface of HepG2 cells with AdCMVLacZ infection. Transforming growth factor-beta1 also enhanced TRAIL expression in HepG2 cells. These results indicate that TRAIL/TRAIL-R apoptotic pathways play important roles in the hepatic cell death during viral infection.

Authors+Show Affiliations

First Department of Internal Medicine, Mie University School of Medicine, Tsu, Mie 514-8507, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16226105

Citation

Saitou, Yukiko, et al. "Involvement of Tumor Necrosis Factor-related Apoptosis-inducing Ligand and Tumor Necrosis Factor-related Apoptosis-inducing Ligand Receptors in Viral Hepatic Diseases." Human Pathology, vol. 36, no. 10, 2005, pp. 1066-73.
Saitou Y, Shiraki K, Fuke H, et al. Involvement of tumor necrosis factor-related apoptosis-inducing ligand and tumor necrosis factor-related apoptosis-inducing ligand receptors in viral hepatic diseases. Hum Pathol. 2005;36(10):1066-73.
Saitou, Y., Shiraki, K., Fuke, H., Inoue, T., Miyashita, K., Yamanaka, Y., Yamaguchi, Y., Yamamoto, N., Ito, K., Sugimoto, K., & Nakano, T. (2005). Involvement of tumor necrosis factor-related apoptosis-inducing ligand and tumor necrosis factor-related apoptosis-inducing ligand receptors in viral hepatic diseases. Human Pathology, 36(10), 1066-73.
Saitou Y, et al. Involvement of Tumor Necrosis Factor-related Apoptosis-inducing Ligand and Tumor Necrosis Factor-related Apoptosis-inducing Ligand Receptors in Viral Hepatic Diseases. Hum Pathol. 2005;36(10):1066-73. PubMed PMID: 16226105.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of tumor necrosis factor-related apoptosis-inducing ligand and tumor necrosis factor-related apoptosis-inducing ligand receptors in viral hepatic diseases. AU - Saitou,Yukiko, AU - Shiraki,Katsuya, AU - Fuke,Hiroyuki, AU - Inoue,Tomoko, AU - Miyashita,Kazumi, AU - Yamanaka,Yutaka, AU - Yamaguchi,Yumi, AU - Yamamoto,Norihik, AU - Ito,Keiichi, AU - Sugimoto,Kazushi, AU - Nakano,Takeshi, PY - 2005/01/05/received PY - 2005/07/21/accepted PY - 2005/10/18/pubmed PY - 2005/12/13/medline PY - 2005/10/18/entrez SP - 1066 EP - 73 JF - Human pathology JO - Hum Pathol VL - 36 IS - 10 N2 - Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces apoptosis in tumor cells, but not in most normal cells. The role of TRAIL in hepatic cell death and hepatic diseases is not well understood. The present study investigated the expression of TRAIL and TRAIL receptors (TRAIL-Rs) in patients with hepatitis C virus infection using immunohistochemistry and examined physiological roles under viral infection in the HepG2 cell line. Staining of TRAIL or TRAIL-Rs was prominent in the cytoplasm and membrane of hepatocytes in the periportal area. Some liver-infiltrating lymphocytes also displayed positive staining for TRAIL. Staining intensity was significantly increased with disease progression, particularly in the periportal area. AdCMVLacZ (Q-BIOgene, Carisbad, Calif) infection was also found to induce apoptosis in HepG2 cells and significantly augment TRAIL-induced apoptosis. Anti-TRAIL antibody significantly inhibited apoptosis induced by AdCMVLacZ infection. Flow cytometry analysis revealed that both TRAIL-R2 and TRAIL were up-regulated on the cell surface of HepG2 cells with AdCMVLacZ infection. Transforming growth factor-beta1 also enhanced TRAIL expression in HepG2 cells. These results indicate that TRAIL/TRAIL-R apoptotic pathways play important roles in the hepatic cell death during viral infection. SN - 0046-8177 UR - https://www.unboundmedicine.com/medline/citation/16226105/Involvement_of_tumor_necrosis_factor_related_apoptosis_inducing_ligand_and_tumor_necrosis_factor_related_apoptosis_inducing_ligand_receptors_in_viral_hepatic_diseases_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0046-8177(05)00385-0 DB - PRIME DP - Unbound Medicine ER -