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Effect of magnesium sulfate on the calcium-stimulated adenosine triphosphatase activity and lipid peroxidation of red blood cell membranes from preeclamptic women.
Biochem Pharmacol. 2005 Nov 25; 70(11):1634-41.BP

Abstract

The effect of the treatment with magnesium sulfate (MgSO(4)) on Ca-ATPase activity and level of lipid peroxidation of red blood cells from preeclamptic pregnant women was examined because it is known that these parameters are affected with preeclampsia. Red cell ghosts from 11 normotensive and 11 preeclamptic pregnant women, before and after treatment with MgSO(4), were assayed for Ca-ATPase activity and level of lipid peroxidation, determined as TBARS or conjugated dienes. It was found that the Ca-ATPase activity is significantly lower and the level of lipid peroxidation is significantly higher in the preeclamptic women with no treatment, as compared to normotensive pregnant women. Both parameters return to normal values after the MgSO(4) therapy. These results can be mimicked by in vitro preincubation with MgSO(4) of intact red blood cells from preeclamptic pregnant women, without any treatment. Our data indicate that MgSO(4) treatment of preeclamptic pregnant women modifies both the Ca-ATPase activity and the level of lipid peroxidation of their red blood cell membranes, reaching values similar to those of normotensive pregnant women. The diminution of the level of lipid peroxidation by MgSO(4), can account for the increase in Ca-ATPase activity.

Authors+Show Affiliations

Laboratorio de Bioenergética Celular, Centro de Biofísica y Bioquímica (CBB), Instituto Venezolano de Investigaciones Científicas (IVIC), A.P. 21827, Caracas 1020A, Venezuela.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16226223

Citation

Abad, Cilia, et al. "Effect of Magnesium Sulfate On the Calcium-stimulated Adenosine Triphosphatase Activity and Lipid Peroxidation of Red Blood Cell Membranes From Preeclamptic Women." Biochemical Pharmacology, vol. 70, no. 11, 2005, pp. 1634-41.
Abad C, Teppa-Garrán A, Proverbio T, et al. Effect of magnesium sulfate on the calcium-stimulated adenosine triphosphatase activity and lipid peroxidation of red blood cell membranes from preeclamptic women. Biochem Pharmacol. 2005;70(11):1634-41.
Abad, C., Teppa-Garrán, A., Proverbio, T., Piñero, S., Proverbio, F., & Marín, R. (2005). Effect of magnesium sulfate on the calcium-stimulated adenosine triphosphatase activity and lipid peroxidation of red blood cell membranes from preeclamptic women. Biochemical Pharmacology, 70(11), 1634-41.
Abad C, et al. Effect of Magnesium Sulfate On the Calcium-stimulated Adenosine Triphosphatase Activity and Lipid Peroxidation of Red Blood Cell Membranes From Preeclamptic Women. Biochem Pharmacol. 2005 Nov 25;70(11):1634-41. PubMed PMID: 16226223.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of magnesium sulfate on the calcium-stimulated adenosine triphosphatase activity and lipid peroxidation of red blood cell membranes from preeclamptic women. AU - Abad,Cilia, AU - Teppa-Garrán,Alejandro, AU - Proverbio,Teresa, AU - Piñero,Sandy, AU - Proverbio,Fulgencio, AU - Marín,Reinaldo, Y1 - 2005/10/13/ PY - 2005/08/11/received PY - 2005/09/13/revised PY - 2005/09/14/accepted PY - 2005/10/18/pubmed PY - 2005/12/21/medline PY - 2005/10/18/entrez SP - 1634 EP - 41 JF - Biochemical pharmacology JO - Biochem Pharmacol VL - 70 IS - 11 N2 - The effect of the treatment with magnesium sulfate (MgSO(4)) on Ca-ATPase activity and level of lipid peroxidation of red blood cells from preeclamptic pregnant women was examined because it is known that these parameters are affected with preeclampsia. Red cell ghosts from 11 normotensive and 11 preeclamptic pregnant women, before and after treatment with MgSO(4), were assayed for Ca-ATPase activity and level of lipid peroxidation, determined as TBARS or conjugated dienes. It was found that the Ca-ATPase activity is significantly lower and the level of lipid peroxidation is significantly higher in the preeclamptic women with no treatment, as compared to normotensive pregnant women. Both parameters return to normal values after the MgSO(4) therapy. These results can be mimicked by in vitro preincubation with MgSO(4) of intact red blood cells from preeclamptic pregnant women, without any treatment. Our data indicate that MgSO(4) treatment of preeclamptic pregnant women modifies both the Ca-ATPase activity and the level of lipid peroxidation of their red blood cell membranes, reaching values similar to those of normotensive pregnant women. The diminution of the level of lipid peroxidation by MgSO(4), can account for the increase in Ca-ATPase activity. SN - 0006-2952 UR - https://www.unboundmedicine.com/medline/citation/16226223/Effect_of_magnesium_sulfate_on_the_calcium_stimulated_adenosine_triphosphatase_activity_and_lipid_peroxidation_of_red_blood_cell_membranes_from_preeclamptic_women_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-2952(05)00599-X DB - PRIME DP - Unbound Medicine ER -