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Osmoregulation, immunolocalization of Na+/K+-ATPase, and ultrastructure of branchial epithelia in the developing brown shrimp, Crangon crangon (Decapoda, Caridea).
Physiol Biochem Zool. 2005 Nov-Dec; 78(6):1017-25.PB

Abstract

Aspects of osmoregulation including salinity tolerance, osmoregulatory capacity, location of transporting epithelia, and the expression of the enzyme Na+/K+-ATPase were investigated in the developing brown shrimp, Crangon crangon (L.), from the North Sea. Early developmental stages and large juveniles were exposed to a wide range of salinities for measurement of hemolymph osmolality and survival rates. In media ranging from 17.0 per thousand to 32.2 per thousand, salinity tolerance was generally high (survival rates: 70%-100%) in all developmental stages, but it decreased in media <10.2 per thousand. Zoeal stages and decapodids slightly hyperregulated at 17.0 per thousand and osmoconformed in media > or =25.5 per thousand. At 10.2 per thousand, these stages showed high mortality, and only juveniles survived at 5.3 per thousand. Juveniles hyperregulated at 10.2 per thousand and 17.0 per thousand, osmoconformed at 25.5 per thousand, and hyporegulated in media > or =32.2 per thousand. Large juveniles hyperregulated also at 5.3 per thousand. Expression of the Na+/K+-ATPase and ion-transporting cells was located through immunofluorescence microscopy and transmission electron microscopy. In zoeae I and VI, a strong immunoreactivity was observed in cells of the inner epithelia of the branchiostegites and in epithelial cells lining the pleurae. Their ultrastructure showed typical features of ion-transporting cells. In decapodids and juveniles, ionocytes and expression of Na+/K+-ATPase remained located in the branchiostegite epithelium, but they disappeared from the pleurae and appeared in the epipodites. In large juveniles, the cells of the gill shaft showed positive immunolabeling and ultrastructural features of ionocytes. In summary, the adult pattern of osmoregulation in C. crangon is accomplished after metamorphosis from a moderately hyperosmoconforming decapodid to an effectively hyper-/hyporegulating juvenile stage. Salinity tolerance and osmoregulatory capacity are closely correlated with the development of ion-transporting cells and the expression of Na+/K+-ATPase.

Authors+Show Affiliations

Biologische Anstalt Helgoland/Stiftung Alfred-Wegener-Institut fur Polar- und Meeresforschung, Meeresstation, D-27498 Helgoland, Germany. ucieluch@awi-bremerhaven.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16228940

Citation

Cieluch, Ude, et al. "Osmoregulation, Immunolocalization of Na+/K+-ATPase, and Ultrastructure of Branchial Epithelia in the Developing Brown Shrimp, Crangon Crangon (Decapoda, Caridea)." Physiological and Biochemical Zoology : PBZ, vol. 78, no. 6, 2005, pp. 1017-25.
Cieluch U, Charmantier G, Grousset E, et al. Osmoregulation, immunolocalization of Na+/K+-ATPase, and ultrastructure of branchial epithelia in the developing brown shrimp, Crangon crangon (Decapoda, Caridea). Physiol Biochem Zool. 2005;78(6):1017-25.
Cieluch, U., Charmantier, G., Grousset, E., Charmantier-Daures, M., & Anger, K. (2005). Osmoregulation, immunolocalization of Na+/K+-ATPase, and ultrastructure of branchial epithelia in the developing brown shrimp, Crangon crangon (Decapoda, Caridea). Physiological and Biochemical Zoology : PBZ, 78(6), 1017-25.
Cieluch U, et al. Osmoregulation, Immunolocalization of Na+/K+-ATPase, and Ultrastructure of Branchial Epithelia in the Developing Brown Shrimp, Crangon Crangon (Decapoda, Caridea). Physiol Biochem Zool. 2005;78(6):1017-25. PubMed PMID: 16228940.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Osmoregulation, immunolocalization of Na+/K+-ATPase, and ultrastructure of branchial epithelia in the developing brown shrimp, Crangon crangon (Decapoda, Caridea). AU - Cieluch,Ude, AU - Charmantier,Guy, AU - Grousset,Evelyse, AU - Charmantier-Daures,Mireille, AU - Anger,Klaus, Y1 - 2005/09/21/ PY - 2004/12/17/accepted PY - 2005/10/18/pubmed PY - 2006/9/14/medline PY - 2005/10/18/entrez SP - 1017 EP - 25 JF - Physiological and biochemical zoology : PBZ JO - Physiol. Biochem. Zool. VL - 78 IS - 6 N2 - Aspects of osmoregulation including salinity tolerance, osmoregulatory capacity, location of transporting epithelia, and the expression of the enzyme Na+/K+-ATPase were investigated in the developing brown shrimp, Crangon crangon (L.), from the North Sea. Early developmental stages and large juveniles were exposed to a wide range of salinities for measurement of hemolymph osmolality and survival rates. In media ranging from 17.0 per thousand to 32.2 per thousand, salinity tolerance was generally high (survival rates: 70%-100%) in all developmental stages, but it decreased in media <10.2 per thousand. Zoeal stages and decapodids slightly hyperregulated at 17.0 per thousand and osmoconformed in media > or =25.5 per thousand. At 10.2 per thousand, these stages showed high mortality, and only juveniles survived at 5.3 per thousand. Juveniles hyperregulated at 10.2 per thousand and 17.0 per thousand, osmoconformed at 25.5 per thousand, and hyporegulated in media > or =32.2 per thousand. Large juveniles hyperregulated also at 5.3 per thousand. Expression of the Na+/K+-ATPase and ion-transporting cells was located through immunofluorescence microscopy and transmission electron microscopy. In zoeae I and VI, a strong immunoreactivity was observed in cells of the inner epithelia of the branchiostegites and in epithelial cells lining the pleurae. Their ultrastructure showed typical features of ion-transporting cells. In decapodids and juveniles, ionocytes and expression of Na+/K+-ATPase remained located in the branchiostegite epithelium, but they disappeared from the pleurae and appeared in the epipodites. In large juveniles, the cells of the gill shaft showed positive immunolabeling and ultrastructural features of ionocytes. In summary, the adult pattern of osmoregulation in C. crangon is accomplished after metamorphosis from a moderately hyperosmoconforming decapodid to an effectively hyper-/hyporegulating juvenile stage. Salinity tolerance and osmoregulatory capacity are closely correlated with the development of ion-transporting cells and the expression of Na+/K+-ATPase. SN - 1522-2152 UR - https://www.unboundmedicine.com/medline/citation/16228940/Osmoregulation_immunolocalization_of_Na+/K+_ATPase_and_ultrastructure_of_branchial_epithelia_in_the_developing_brown_shrimp_Crangon_crangon__Decapoda_Caridea__ L2 - http://dx.doi.org/10.1086/432856 DB - PRIME DP - Unbound Medicine ER -