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Biomarkers of neutrophilic inflammation in exhaled air of cystic fibrosis children with bacterial airway infections.
Pediatr Pulmonol. 2005 Dec; 40(6):494-9.PP

Abstract

Leukotriene B(4) (LTB(4)) and interleukin-8 (IL-8) are inflammatory mediators involved in the neutrophil response to pulmonary bacterial colonization in cystic fibrosis (CF). The aim of this study was to investigate whether the LTB(4) and IL-8 levels in exhaled breath condensate (EBC) could be related to the type of bacterial colonization in CF patients. The pH level in EBC was analyzed as an estimate of airway acidification. Forty children were evaluated: 10 CF patients with P. aeruginosa, 10 CF patients with S. aureus, 10 not colonized CF patients, and 10 healthy children. LTB(4) and IL-8 in EBC were analyzed by specific enzyme immunoassay kits (EIA). The pH of EBC was measured with a pH-meter after deareation by bubbling with argon. Exhaled LTB(4) was higher in CF children with P. aeruginosa compared to those with S. aureus (P < 0.01), not colonized (P < 0.001), and healthy children (P < 0.01). Exhaled IL-8 was elevated in CF patients colonized by P. aeruginosa compared with other subgroups (vs. not colonized, P < 0.05; vs. healthy children, P < 0.001). IL-8 levels were higher in CF children with S. aureus than in healthy children (P < 0.05). There was an increase in IL-8 levels in not colonized CF patients compared with healthy children (P < 0.05). EBC pH was higher in healthy children compared to CF patients not colonized (P < 0.05). Our data suggest that EBC is suitable for evaluating neutrophil inflammatory mediators (LTB(4), IL-8, and pH) involved in the response to pulmonary bacterial colonization in CF children.

Authors+Show Affiliations

Department of Pediatrics, University of Verona, Verona, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16229003

Citation

Bodini, Alessandro, et al. "Biomarkers of Neutrophilic Inflammation in Exhaled Air of Cystic Fibrosis Children With Bacterial Airway Infections." Pediatric Pulmonology, vol. 40, no. 6, 2005, pp. 494-9.
Bodini A, D'Orazio C, Peroni D, et al. Biomarkers of neutrophilic inflammation in exhaled air of cystic fibrosis children with bacterial airway infections. Pediatr Pulmonol. 2005;40(6):494-9.
Bodini, A., D'Orazio, C., Peroni, D., Corradi, M., Folesani, G., Baraldi, E., Assael, B. M., Boner, A., & Piacentini, G. L. (2005). Biomarkers of neutrophilic inflammation in exhaled air of cystic fibrosis children with bacterial airway infections. Pediatric Pulmonology, 40(6), 494-9.
Bodini A, et al. Biomarkers of Neutrophilic Inflammation in Exhaled Air of Cystic Fibrosis Children With Bacterial Airway Infections. Pediatr Pulmonol. 2005;40(6):494-9. PubMed PMID: 16229003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biomarkers of neutrophilic inflammation in exhaled air of cystic fibrosis children with bacterial airway infections. AU - Bodini,Alessandro, AU - D'Orazio,Ciro, AU - Peroni,Diego, AU - Corradi,Massimo, AU - Folesani,Giuseppina, AU - Baraldi,Eugenio, AU - Assael,Barouk M, AU - Boner,Attilio, AU - Piacentini,Giorgio L, PY - 2005/10/18/pubmed PY - 2006/3/24/medline PY - 2005/10/18/entrez SP - 494 EP - 9 JF - Pediatric pulmonology JO - Pediatr Pulmonol VL - 40 IS - 6 N2 - Leukotriene B(4) (LTB(4)) and interleukin-8 (IL-8) are inflammatory mediators involved in the neutrophil response to pulmonary bacterial colonization in cystic fibrosis (CF). The aim of this study was to investigate whether the LTB(4) and IL-8 levels in exhaled breath condensate (EBC) could be related to the type of bacterial colonization in CF patients. The pH level in EBC was analyzed as an estimate of airway acidification. Forty children were evaluated: 10 CF patients with P. aeruginosa, 10 CF patients with S. aureus, 10 not colonized CF patients, and 10 healthy children. LTB(4) and IL-8 in EBC were analyzed by specific enzyme immunoassay kits (EIA). The pH of EBC was measured with a pH-meter after deareation by bubbling with argon. Exhaled LTB(4) was higher in CF children with P. aeruginosa compared to those with S. aureus (P < 0.01), not colonized (P < 0.001), and healthy children (P < 0.01). Exhaled IL-8 was elevated in CF patients colonized by P. aeruginosa compared with other subgroups (vs. not colonized, P < 0.05; vs. healthy children, P < 0.001). IL-8 levels were higher in CF children with S. aureus than in healthy children (P < 0.05). There was an increase in IL-8 levels in not colonized CF patients compared with healthy children (P < 0.05). EBC pH was higher in healthy children compared to CF patients not colonized (P < 0.05). Our data suggest that EBC is suitable for evaluating neutrophil inflammatory mediators (LTB(4), IL-8, and pH) involved in the response to pulmonary bacterial colonization in CF children. SN - 8755-6863 UR - https://www.unboundmedicine.com/medline/citation/16229003/Biomarkers_of_neutrophilic_inflammation_in_exhaled_air_of_cystic_fibrosis_children_with_bacterial_airway_infections_ DB - PRIME DP - Unbound Medicine ER -