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Paraoxonase 1 (PON1) is a more potent antioxidant and stimulant of macrophage cholesterol efflux, when present in HDL than in lipoprotein-deficient serum: relevance to diabetes.
Atherosclerosis. 2006 Jul; 187(1):74-81.A

Abstract

The present study analyzed serum paraoxonase 1 (PON1) distribution among HDL and lipoprotein-deficient serum (LPDS) in atherosclerotic patients, and compared PON1 biological functions in these fractions. Serum HDL and LPDS fractions were isolated from control healthy subjects, diabetic and hypercholesterolemic patients. PON1 activities and protein in HDL/LPDS, as well as its ability to protect against lipid peroxidation and to stimulate HDL/LPDS-mediated macrophage cholesterol efflux were measured. In LPDS from controls, PON1 protein and a significant paraoxonase activity were found, whereas arylesterase and lactonase activities were substantially reduced compared to HDL, by 78% and 88%, respectively. In diabetic patients, PON1 protein and paraoxonase activity in HDL were significantly decreased by 2.8- and 1.7-fold, respectively, compared with controls' HDL. In parallel, in these patient's LPDS, PON1 protein and paraoxonase activity were markedly increased by 3.7- and 1.7-fold, respectively, compared with controls' LPDS. PON1 in HDL (but not PON1 in LPDS) significantly decreased AAPH-induced lipid peroxides formation by 33%, and increased macrophage cholesterol efflux by 31%. We conclude that PON1 is less antiatherogenic when present in LPDS than in HDL. The abnormal serum PON1 distribution in diabetic patients, could be responsible for the accelerated atherosclerosis development in these patients.

Authors+Show Affiliations

The Lipid Research Laboratory, Technion Faculty of Medicine, The Rappaport Family Institute for Research in the Medical Sciences and Rambam Medical Center, 31096 Haifa, Israel.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16229851

Citation

Rosenblat, Mira, et al. "Paraoxonase 1 (PON1) Is a More Potent Antioxidant and Stimulant of Macrophage Cholesterol Efflux, when Present in HDL Than in Lipoprotein-deficient Serum: Relevance to Diabetes." Atherosclerosis, vol. 187, no. 1, 2006, pp. 74-81.
Rosenblat M, Karry R, Aviram M. Paraoxonase 1 (PON1) is a more potent antioxidant and stimulant of macrophage cholesterol efflux, when present in HDL than in lipoprotein-deficient serum: relevance to diabetes. Atherosclerosis. 2006;187(1):74-81.
Rosenblat, M., Karry, R., & Aviram, M. (2006). Paraoxonase 1 (PON1) is a more potent antioxidant and stimulant of macrophage cholesterol efflux, when present in HDL than in lipoprotein-deficient serum: relevance to diabetes. Atherosclerosis, 187(1), 74-81.
Rosenblat M, Karry R, Aviram M. Paraoxonase 1 (PON1) Is a More Potent Antioxidant and Stimulant of Macrophage Cholesterol Efflux, when Present in HDL Than in Lipoprotein-deficient Serum: Relevance to Diabetes. Atherosclerosis. 2006;187(1):74-81. PubMed PMID: 16229851.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Paraoxonase 1 (PON1) is a more potent antioxidant and stimulant of macrophage cholesterol efflux, when present in HDL than in lipoprotein-deficient serum: relevance to diabetes. AU - Rosenblat,Mira, AU - Karry,Rachel, AU - Aviram,Michael, Y1 - 2005/10/17/ PY - 2005/04/14/received PY - 2005/07/12/revised PY - 2005/08/17/accepted PY - 2005/10/19/pubmed PY - 2007/1/18/medline PY - 2005/10/19/entrez SP - 74 EP - 81 JF - Atherosclerosis JO - Atherosclerosis VL - 187 IS - 1 N2 - The present study analyzed serum paraoxonase 1 (PON1) distribution among HDL and lipoprotein-deficient serum (LPDS) in atherosclerotic patients, and compared PON1 biological functions in these fractions. Serum HDL and LPDS fractions were isolated from control healthy subjects, diabetic and hypercholesterolemic patients. PON1 activities and protein in HDL/LPDS, as well as its ability to protect against lipid peroxidation and to stimulate HDL/LPDS-mediated macrophage cholesterol efflux were measured. In LPDS from controls, PON1 protein and a significant paraoxonase activity were found, whereas arylesterase and lactonase activities were substantially reduced compared to HDL, by 78% and 88%, respectively. In diabetic patients, PON1 protein and paraoxonase activity in HDL were significantly decreased by 2.8- and 1.7-fold, respectively, compared with controls' HDL. In parallel, in these patient's LPDS, PON1 protein and paraoxonase activity were markedly increased by 3.7- and 1.7-fold, respectively, compared with controls' LPDS. PON1 in HDL (but not PON1 in LPDS) significantly decreased AAPH-induced lipid peroxides formation by 33%, and increased macrophage cholesterol efflux by 31%. We conclude that PON1 is less antiatherogenic when present in LPDS than in HDL. The abnormal serum PON1 distribution in diabetic patients, could be responsible for the accelerated atherosclerosis development in these patients. SN - 0021-9150 UR - https://www.unboundmedicine.com/medline/citation/16229851/Paraoxonase_1__PON1__is_a_more_potent_antioxidant_and_stimulant_of_macrophage_cholesterol_efflux_when_present_in_HDL_than_in_lipoprotein_deficient_serum:_relevance_to_diabetes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9150(05)00546-0 DB - PRIME DP - Unbound Medicine ER -