Tags

Type your tag names separated by a space and hit enter

Detection of FLT3 gene and FLT3/ITD mutation by polymerase chain reaction-single-strand conformation polymorphism in patients with acute lymphoblastic leukemia.
Di Yi Jun Yi Da Xue Xue Bao. 2005 Oct; 25(10):1207-10.DY

Abstract

OBJECTIVE

To analyze Fms-like tyrosine kinase 3 (FLT3) gene and FLT3 internal tandem duplication (ITD) mutation in acute lymphoblastic leukemia (ALL) patients of different immunological subtypes.

METHODS

Polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) was used to detect FLT3 gene and FLT3/ITD mutation in 63 ALL cases.

RESULTS

Among the 63 ALL cases, FLT3 gene was detected in 41 (61.5%) cases. The positivity rate of FLT3 gene in pre-pre B-lineage ALL, pre-B-ALL, B-lineage ALL and T-lineage ALL cases were 93.3% (14/15), 77.8% (14/18), 41.7% (5/12) and 28.6% (4/14), respectively. The positivity rate of FLT3 gene was significantly higher in pre-pre B-ALL/pre B-ALL subtypes (84.8%) than in B-ALL subtypes (41.7%, P<0.005), and the rate was significantly higher in B-ALL subtypes (73.3%) than in T-ALL subtypes (28.6%, P<0.001). Two cases (3.2%) were found to have FLT3/ITD mutation, which were also positive for myeloid antigen expression and diagnosed as acute mixed-lineage leukemia, showing leukocytosis and high percentage of bone marrow blast cells with poor prognosis.

CONCLUSIONS

FLT3 gene can be detected in both B-and T-lineage ALL patients, but more frequently in the former. In B-lineage ALL patients, FLT3 gene is more frequent in cases with undifferentiated than those with differentiated blast cells. FLT3/ITD is rarely detected in ALL patients and FLT3/ITD mutation detection might be helpful to identify the genotypes and evaluate the prognosis of acute leukemia.

Authors+Show Affiliations

Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. xbzj@fimmu.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16234090

Citation

Xu, Bing, et al. "Detection of FLT3 Gene and FLT3/ITD Mutation By Polymerase Chain Reaction-single-strand Conformation Polymorphism in Patients With Acute Lymphoblastic Leukemia." Di 1 Jun Yi Da Xue Xue Bao = Academic Journal of the First Medical College of PLA, vol. 25, no. 10, 2005, pp. 1207-10.
Xu B, Li L, Tang JH, et al. Detection of FLT3 gene and FLT3/ITD mutation by polymerase chain reaction-single-strand conformation polymorphism in patients with acute lymphoblastic leukemia. Di Yi Jun Yi Da Xue Xue Bao. 2005;25(10):1207-10.
Xu, B., Li, L., Tang, J. H., & Zhou, S. Y. (2005). Detection of FLT3 gene and FLT3/ITD mutation by polymerase chain reaction-single-strand conformation polymorphism in patients with acute lymphoblastic leukemia. Di 1 Jun Yi Da Xue Xue Bao = Academic Journal of the First Medical College of PLA, 25(10), 1207-10.
Xu B, et al. Detection of FLT3 Gene and FLT3/ITD Mutation By Polymerase Chain Reaction-single-strand Conformation Polymorphism in Patients With Acute Lymphoblastic Leukemia. Di Yi Jun Yi Da Xue Xue Bao. 2005;25(10):1207-10. PubMed PMID: 16234090.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of FLT3 gene and FLT3/ITD mutation by polymerase chain reaction-single-strand conformation polymorphism in patients with acute lymphoblastic leukemia. AU - Xu,Bing, AU - Li,Lin, AU - Tang,Jia-hong, AU - Zhou,Shu-yun, PY - 2005/10/20/pubmed PY - 2007/12/18/medline PY - 2005/10/20/entrez SP - 1207 EP - 10 JF - Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA JO - Di Yi Jun Yi Da Xue Xue Bao VL - 25 IS - 10 N2 - OBJECTIVE: To analyze Fms-like tyrosine kinase 3 (FLT3) gene and FLT3 internal tandem duplication (ITD) mutation in acute lymphoblastic leukemia (ALL) patients of different immunological subtypes. METHODS: Polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) was used to detect FLT3 gene and FLT3/ITD mutation in 63 ALL cases. RESULTS: Among the 63 ALL cases, FLT3 gene was detected in 41 (61.5%) cases. The positivity rate of FLT3 gene in pre-pre B-lineage ALL, pre-B-ALL, B-lineage ALL and T-lineage ALL cases were 93.3% (14/15), 77.8% (14/18), 41.7% (5/12) and 28.6% (4/14), respectively. The positivity rate of FLT3 gene was significantly higher in pre-pre B-ALL/pre B-ALL subtypes (84.8%) than in B-ALL subtypes (41.7%, P<0.005), and the rate was significantly higher in B-ALL subtypes (73.3%) than in T-ALL subtypes (28.6%, P<0.001). Two cases (3.2%) were found to have FLT3/ITD mutation, which were also positive for myeloid antigen expression and diagnosed as acute mixed-lineage leukemia, showing leukocytosis and high percentage of bone marrow blast cells with poor prognosis. CONCLUSIONS: FLT3 gene can be detected in both B-and T-lineage ALL patients, but more frequently in the former. In B-lineage ALL patients, FLT3 gene is more frequent in cases with undifferentiated than those with differentiated blast cells. FLT3/ITD is rarely detected in ALL patients and FLT3/ITD mutation detection might be helpful to identify the genotypes and evaluate the prognosis of acute leukemia. SN - 1000-2588 UR - https://www.unboundmedicine.com/medline/citation/16234090/Detection_of_FLT3_gene_and_FLT3/ITD_mutation_by_polymerase_chain_reaction_single_strand_conformation_polymorphism_in_patients_with_acute_lymphoblastic_leukemia_ L2 - https://www.diseaseinfosearch.org/result/188 DB - PRIME DP - Unbound Medicine ER -