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Upregulation of T-bet expression in peripheral blood mononuclear cells during Vogt-Koyanagi-Harada disease.
Br J Ophthalmol. 2005 Nov; 89(11):1410-2.BJ

Abstract

AIM

To test the hypothesis that T-bet expression is altered in patients with Vogt-Koyanagi-Harada (VKH) disease.

METHODS

Peripheral blood was withdrawn from 16 VKH patients before and after immunosuppressive treatment and from 16 healthy individuals. IFN-gamma, IL-2, and IL-4 in the serum and the supernatants of peripheral blood mononuclear cells (PBMC) cultured with or without phytohaemagglutinin (PHA) were measured by ELISA. T-bet mRNA and protein expression in PBMC cultured with or without PHA was detected by RT-PCR and western blot, respectively.

RESULTS

The level of IFN-gamma, but not IL-2 and IL-4, was significantly higher in the supernatants of stimulated PBMC in patients than in controls. A significantly increased T-bet mRNA was found in VKH patients during an active uveitis episode, but not in quiescent patients, compared to controls. T-bet protein was detectable in VKH patients during an active uveitis episode, but not in quiescent patients nor in the healthy controls. Stimulation of PBMC with PHA resulted in a marked upregulation of T-bet mRNA and protein expression for both patients and controls with no significant difference between the two groups.

CONCLUSIONS

Upregulation of T-bet may be associated with the development of a Th1 mediated immune response in VKH disease.

Authors+Show Affiliations

Zhongshan Ophthalmic Center, Sun Yat-sen University, 54 Xianlie Road, Guangzhou 510060, P R China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16234441

Citation

Li, B, et al. "Upregulation of T-bet Expression in Peripheral Blood Mononuclear Cells During Vogt-Koyanagi-Harada Disease." The British Journal of Ophthalmology, vol. 89, no. 11, 2005, pp. 1410-2.
Li B, Yang P, Zhou H, et al. Upregulation of T-bet expression in peripheral blood mononuclear cells during Vogt-Koyanagi-Harada disease. Br J Ophthalmol. 2005;89(11):1410-2.
Li, B., Yang, P., Zhou, H., Huang, X., Jin, H., Chu, L., Gao, Y., Zhu, L., & Kijlstra, A. (2005). Upregulation of T-bet expression in peripheral blood mononuclear cells during Vogt-Koyanagi-Harada disease. The British Journal of Ophthalmology, 89(11), 1410-2.
Li B, et al. Upregulation of T-bet Expression in Peripheral Blood Mononuclear Cells During Vogt-Koyanagi-Harada Disease. Br J Ophthalmol. 2005;89(11):1410-2. PubMed PMID: 16234441.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Upregulation of T-bet expression in peripheral blood mononuclear cells during Vogt-Koyanagi-Harada disease. AU - Li,B, AU - Yang,P, AU - Zhou,H, AU - Huang,X, AU - Jin,H, AU - Chu,L, AU - Gao,Y, AU - Zhu,L, AU - Kijlstra,A, PY - 2005/10/20/pubmed PY - 2005/12/13/medline PY - 2005/10/20/entrez SP - 1410 EP - 2 JF - The British journal of ophthalmology JO - Br J Ophthalmol VL - 89 IS - 11 N2 - AIM: To test the hypothesis that T-bet expression is altered in patients with Vogt-Koyanagi-Harada (VKH) disease. METHODS: Peripheral blood was withdrawn from 16 VKH patients before and after immunosuppressive treatment and from 16 healthy individuals. IFN-gamma, IL-2, and IL-4 in the serum and the supernatants of peripheral blood mononuclear cells (PBMC) cultured with or without phytohaemagglutinin (PHA) were measured by ELISA. T-bet mRNA and protein expression in PBMC cultured with or without PHA was detected by RT-PCR and western blot, respectively. RESULTS: The level of IFN-gamma, but not IL-2 and IL-4, was significantly higher in the supernatants of stimulated PBMC in patients than in controls. A significantly increased T-bet mRNA was found in VKH patients during an active uveitis episode, but not in quiescent patients, compared to controls. T-bet protein was detectable in VKH patients during an active uveitis episode, but not in quiescent patients nor in the healthy controls. Stimulation of PBMC with PHA resulted in a marked upregulation of T-bet mRNA and protein expression for both patients and controls with no significant difference between the two groups. CONCLUSIONS: Upregulation of T-bet may be associated with the development of a Th1 mediated immune response in VKH disease. SN - 0007-1161 UR - https://www.unboundmedicine.com/medline/citation/16234441/Upregulation_of_T_bet_expression_in_peripheral_blood_mononuclear_cells_during_Vogt_Koyanagi_Harada_disease_ L2 - http://bjo.bmj.com/cgi/pmidlookup?view=long&pmid=16234441 DB - PRIME DP - Unbound Medicine ER -