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[Effects of hepatocyte growth factor on TGF-beta1 triggered tubular epithelial-myofibroblast transdifferentiation by CTGF in vitro].
Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Sep; 36(5):653-6, 691.SD

Abstract

OBJECTIVE

To observe the effects of hepatocyte growth factor (HGF) on TGF-beta1 triggered tubular epithelial-myofibroblast transdifferentiation (TEMT) and on the expression of connective tissue growth factor (CTGF).

METHODS

The morphology of transdifferentiate tubular cells was observed using phase-contrast microscopy and scanning electron microscopy. alpha-SMA was assessed by immunohistochemistry and semiquantified by mean intergrated opitical density (IOD). The level of fibronectin (FN) in the culture supernatant was measured by ELISA. CTGF mRNA expression was examined by RT-PCR.

RESULTS

The TGF-beta1-induced TEMT characterized by expression of alpha-SMA was shown by immunohistochemistry. TGF-beta1 was also shown to stimulate the secretion of FN in cultured supernatant and the CTGF mRNA expression of NRK52E cells. There was no statistically significant difference between HGF-treated groups and control group in the result of alpha-SMA immunostaining and the level of FN, except that CTGF mRNA expression was slightly increased in the HGF-treated groups. The addition of HGF inhibited the TGF-beta1-induced TEMT, the secretion of FN, and the CTGF expression of NRK52E cells, there was a significant correlation between the expression of CTGF and the expression of alpha-SMA.

CONCLUSION

HGF could block TEMT and FN secretion triggered by TGF-beta1, which implies that HGF could participate in renal interstitial fibrosis as a negative regulator. The negative regulation of transdifferentiation of HGF may be partially achieved by attenuation of CTGF expression.

Authors+Show Affiliations

Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16235529

Citation

Zhong, Hui, et al. "[Effects of Hepatocyte Growth Factor On TGF-beta1 Triggered Tubular Epithelial-myofibroblast Transdifferentiation By CTGF in Vitro]." Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition, vol. 36, no. 5, 2005, pp. 653-6, 691.
Zhong H, Fan JM, Li Z, et al. [Effects of hepatocyte growth factor on TGF-beta1 triggered tubular epithelial-myofibroblast transdifferentiation by CTGF in vitro]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2005;36(5):653-6, 691.
Zhong, H., Fan, J. M., Li, Z., Liu, F., Yang, L. C., Ji, L., Sha, Z. H., & Ma, X. Y. (2005). [Effects of hepatocyte growth factor on TGF-beta1 triggered tubular epithelial-myofibroblast transdifferentiation by CTGF in vitro]. Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition, 36(5), 653-6, 691.
Zhong H, et al. [Effects of Hepatocyte Growth Factor On TGF-beta1 Triggered Tubular Epithelial-myofibroblast Transdifferentiation By CTGF in Vitro]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2005;36(5):653-6, 691. PubMed PMID: 16235529.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of hepatocyte growth factor on TGF-beta1 triggered tubular epithelial-myofibroblast transdifferentiation by CTGF in vitro]. AU - Zhong,Hui, AU - Fan,Jun-Ming, AU - Li,Zi, AU - Liu,Fang, AU - Yang,Li-Chuan, AU - Ji,Ling, AU - Sha,Zhao-Hui, AU - Ma,Xing-Yi, PY - 2005/10/21/pubmed PY - 2006/9/20/medline PY - 2005/10/21/entrez SP - 653-6, 691 JF - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition JO - Sichuan Da Xue Xue Bao Yi Xue Ban VL - 36 IS - 5 N2 - OBJECTIVE: To observe the effects of hepatocyte growth factor (HGF) on TGF-beta1 triggered tubular epithelial-myofibroblast transdifferentiation (TEMT) and on the expression of connective tissue growth factor (CTGF). METHODS: The morphology of transdifferentiate tubular cells was observed using phase-contrast microscopy and scanning electron microscopy. alpha-SMA was assessed by immunohistochemistry and semiquantified by mean intergrated opitical density (IOD). The level of fibronectin (FN) in the culture supernatant was measured by ELISA. CTGF mRNA expression was examined by RT-PCR. RESULTS: The TGF-beta1-induced TEMT characterized by expression of alpha-SMA was shown by immunohistochemistry. TGF-beta1 was also shown to stimulate the secretion of FN in cultured supernatant and the CTGF mRNA expression of NRK52E cells. There was no statistically significant difference between HGF-treated groups and control group in the result of alpha-SMA immunostaining and the level of FN, except that CTGF mRNA expression was slightly increased in the HGF-treated groups. The addition of HGF inhibited the TGF-beta1-induced TEMT, the secretion of FN, and the CTGF expression of NRK52E cells, there was a significant correlation between the expression of CTGF and the expression of alpha-SMA. CONCLUSION: HGF could block TEMT and FN secretion triggered by TGF-beta1, which implies that HGF could participate in renal interstitial fibrosis as a negative regulator. The negative regulation of transdifferentiation of HGF may be partially achieved by attenuation of CTGF expression. SN - 1672-173X UR - https://www.unboundmedicine.com/medline/citation/16235529/[Effects_of_hepatocyte_growth_factor_on_TGF_beta1_triggered_tubular_epithelial_myofibroblast_transdifferentiation_by_CTGF_in_vitro]_ L2 - https://antibodies.cancer.gov/detail/CPTC-IL6-1 DB - PRIME DP - Unbound Medicine ER -