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[Effect of high glucose on the expression of fibronectin and plasminogen activator inhibitor-1 in human peritoneal mesothelial cells].
Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Sep; 36(5):700-2.SD

Abstract

OBJECTIVE

To evaluate the effect of high glucose on the expression of fibronectin (FN) and plasminogen activator inhibitor-1 (PAI-1) in human peritoneal mesothelial cells (HPMC).

METHODS

The cultured HPMC were exposed to the culture medium containing different concentrations of glucose (1.5%, 2.5%, 4.25%) for 6, 12, 24, 48 hours, and normal culture medium was used as control. FN and PAI-1 in supernatant were detected by enzyme-linked immunoassay(ELISA).

RESULTS

The glucose at 1. 5%, 2.5%, 4.25% resulted in a time- and concentration-dependent increase in FN expression in HPMC(P < 0.05). The glucose at 2.5%, 4.25% increased PAI-1 expression in HPMC (P < 0.05), and the effect was time- and concentration-dependent. PAI-1 expression was not affected by 1.5% glucose (P > 0.05).

CONCLUSION

High glucose increases the expression of FN and PAI-1 in HPMC, thus playing a part in the progression of peritoneal fibrosis.

Authors+Show Affiliations

Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

16235542

Citation

Ji, Ling, et al. "[Effect of High Glucose On the Expression of Fibronectin and Plasminogen Activator Inhibitor-1 in Human Peritoneal Mesothelial Cells]." Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition, vol. 36, no. 5, 2005, pp. 700-2.
Ji L, Zhuang B, Yang LC, et al. [Effect of high glucose on the expression of fibronectin and plasminogen activator inhibitor-1 in human peritoneal mesothelial cells]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2005;36(5):700-2.
Ji, L., Zhuang, B., Yang, L. C., Liu, X. J., & Liu, X. R. (2005). [Effect of high glucose on the expression of fibronectin and plasminogen activator inhibitor-1 in human peritoneal mesothelial cells]. Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition, 36(5), 700-2.
Ji L, et al. [Effect of High Glucose On the Expression of Fibronectin and Plasminogen Activator Inhibitor-1 in Human Peritoneal Mesothelial Cells]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2005;36(5):700-2. PubMed PMID: 16235542.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of high glucose on the expression of fibronectin and plasminogen activator inhibitor-1 in human peritoneal mesothelial cells]. AU - Ji,Ling, AU - Zhuang,Bin, AU - Yang,Li-Chuan, AU - Liu,Xiao-Jing, AU - Liu,Xian-Rong, PY - 2005/10/21/pubmed PY - 2006/9/20/medline PY - 2005/10/21/entrez SP - 700 EP - 2 JF - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition JO - Sichuan Da Xue Xue Bao Yi Xue Ban VL - 36 IS - 5 N2 - OBJECTIVE: To evaluate the effect of high glucose on the expression of fibronectin (FN) and plasminogen activator inhibitor-1 (PAI-1) in human peritoneal mesothelial cells (HPMC). METHODS: The cultured HPMC were exposed to the culture medium containing different concentrations of glucose (1.5%, 2.5%, 4.25%) for 6, 12, 24, 48 hours, and normal culture medium was used as control. FN and PAI-1 in supernatant were detected by enzyme-linked immunoassay(ELISA). RESULTS: The glucose at 1. 5%, 2.5%, 4.25% resulted in a time- and concentration-dependent increase in FN expression in HPMC(P < 0.05). The glucose at 2.5%, 4.25% increased PAI-1 expression in HPMC (P < 0.05), and the effect was time- and concentration-dependent. PAI-1 expression was not affected by 1.5% glucose (P > 0.05). CONCLUSION: High glucose increases the expression of FN and PAI-1 in HPMC, thus playing a part in the progression of peritoneal fibrosis. SN - 1672-173X UR - https://www.unboundmedicine.com/medline/citation/16235542/[Effect_of_high_glucose_on_the_expression_of_fibronectin_and_plasminogen_activator_inhibitor_1_in_human_peritoneal_mesothelial_cells]_ DB - PRIME DP - Unbound Medicine ER -