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Responses of microglia in vitro to the gram-positive bacterial component, lipoteichoic acid.
J Neurosci Res 2005; 82(4):515-24JN

Abstract

An increase in incidence and severity of gram-positive infections has emerged in the past decade. In this regard, attention has been focused recently on immune responses of microglial cells in the central nervous system to gram-positive bacteria. The underlying immunological and cellular events in microglial activation induced by specific bacterial toxin of gram-positive bacteria, however, have not yet been clarified fully. This study reports that a simple cell wall product, lipoteichoic acid (LTA), derived from gram-positive bacteria (Staphylococcus aureus) could trigger microglial activation in vitro. Microglia challenged with LTA showed intense ruffling of plasma membrane in the form of lamellipodia or rounded up forming cell aggregates. MTT assay and Western blot analysis with anti-proliferating cell nuclear antigen antibody showed a significant microglial proliferation that may be induced at the later phases of LTA treatment with low doses but at the early period with a high dose. Concentrated LTA also caused apoptotic death of cultured microglia showing fragmented nuclei and increased expression of annexin V or caspase 3. In response to LTA, isolated microglia increased the expression of inducible nitric oxide synthase and major histocompatibility complex class II antigen. Microglial LTA receptors such as CD14 molecule, complement receptor type 3, and macrophage scavenger receptor were upregulated concurrently. In conclusion, staphylococcal LTA can exert an immunomodulatory effect on microglial morphology, cell cycle, and immunomolecules, including its receptors.

Authors+Show Affiliations

Department of Anatomy, National Cheng Kung University Medical College, Tainan, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16237723

Citation

Jiang-Shieh, Ya-Fen, et al. "Responses of Microglia in Vitro to the Gram-positive Bacterial Component, Lipoteichoic Acid." Journal of Neuroscience Research, vol. 82, no. 4, 2005, pp. 515-24.
Jiang-Shieh YF, Yeh KY, Wei IH, et al. Responses of microglia in vitro to the gram-positive bacterial component, lipoteichoic acid. J Neurosci Res. 2005;82(4):515-24.
Jiang-Shieh, Y. F., Yeh, K. Y., Wei, I. H., Chang, C. Y., Chien, H. F., Tsai, R. Y., ... Wu, C. H. (2005). Responses of microglia in vitro to the gram-positive bacterial component, lipoteichoic acid. Journal of Neuroscience Research, 82(4), pp. 515-24.
Jiang-Shieh YF, et al. Responses of Microglia in Vitro to the Gram-positive Bacterial Component, Lipoteichoic Acid. J Neurosci Res. 2005 Nov 15;82(4):515-24. PubMed PMID: 16237723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Responses of microglia in vitro to the gram-positive bacterial component, lipoteichoic acid. AU - Jiang-Shieh,Ya-Fen, AU - Yeh,Kuei-Ying, AU - Wei,I-Hua, AU - Chang,Chiu-Yun, AU - Chien,Hsiung-Fei, AU - Tsai,Ru-Yin, AU - Chang,Min-Lin, AU - Lee,Ai-Wei, AU - Pai,Man-Hui, AU - Wu,Ching-Hsiang, PY - 2005/10/21/pubmed PY - 2006/2/17/medline PY - 2005/10/21/entrez SP - 515 EP - 24 JF - Journal of neuroscience research JO - J. Neurosci. Res. VL - 82 IS - 4 N2 - An increase in incidence and severity of gram-positive infections has emerged in the past decade. In this regard, attention has been focused recently on immune responses of microglial cells in the central nervous system to gram-positive bacteria. The underlying immunological and cellular events in microglial activation induced by specific bacterial toxin of gram-positive bacteria, however, have not yet been clarified fully. This study reports that a simple cell wall product, lipoteichoic acid (LTA), derived from gram-positive bacteria (Staphylococcus aureus) could trigger microglial activation in vitro. Microglia challenged with LTA showed intense ruffling of plasma membrane in the form of lamellipodia or rounded up forming cell aggregates. MTT assay and Western blot analysis with anti-proliferating cell nuclear antigen antibody showed a significant microglial proliferation that may be induced at the later phases of LTA treatment with low doses but at the early period with a high dose. Concentrated LTA also caused apoptotic death of cultured microglia showing fragmented nuclei and increased expression of annexin V or caspase 3. In response to LTA, isolated microglia increased the expression of inducible nitric oxide synthase and major histocompatibility complex class II antigen. Microglial LTA receptors such as CD14 molecule, complement receptor type 3, and macrophage scavenger receptor were upregulated concurrently. In conclusion, staphylococcal LTA can exert an immunomodulatory effect on microglial morphology, cell cycle, and immunomolecules, including its receptors. SN - 0360-4012 UR - https://www.unboundmedicine.com/medline/citation/16237723/Responses_of_microglia_in_vitro_to_the_gram_positive_bacterial_component_lipoteichoic_acid_ L2 - https://doi.org/10.1002/jnr.20663 DB - PRIME DP - Unbound Medicine ER -