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Analysis of developmental potentials of dental pulp in vitro using GFP transgenes.
Orthod Craniofac Res. 2005 Nov; 8(4):252-8.OC

Abstract

BACKGROUND

In recent years there has been increasing progress in identifying stem cells from adult tissues and their potential application for tooth replacement/regeneration. Our previous in vivo studies show that pOBCol3.6GFP and pOBCol2.3GFP transgenic animals provide a unique model to gain insight into progenitor/stem cells in the dental pulp capable of giving rise to odontoblasts.

OBJECTIVES

To characterize the behavior of dental pulp cells derived from pOBCol3.6GFP animals in vitro.

EXPERIMENTAL DESIGN

Primary cultures were established from the coronal portions of the pulps isolated first molars from 5-day-old pOBCol3.6GFP heterozygous mice and grown for 21 days. In these cultures proliferation, clonogenic capacity, activation of 3.6-GFP and mineralization were examined.

RESULTS

Our observations show that dental pulp cells derived from 3.6-GFP contain a population of proliferative, clonogenic cells with the ability to mineralize. We also show the stage specific activation/upregulation of 3.6-GFP in primary cultures derived from dental pulp. In these cultures, expression of Col1a1-3.6-GFP occurs prior to the appearance of mineralized nodules and is unregulated in mineralized nodules.

CONCLUSIONS

Col1a1-GFP transgenes appear to fulfill many of the requirements of a marker gene for cell lineage studies in intact tooth and primary cultures derived from dental pulp.

Authors+Show Affiliations

Department of Pediatric Dentistry, School of Dental Medicine, University of Connecticut Health Center, Farmington, 06030, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16238605

Citation

Balic, A, and M Mina. "Analysis of Developmental Potentials of Dental Pulp in Vitro Using GFP Transgenes." Orthodontics & Craniofacial Research, vol. 8, no. 4, 2005, pp. 252-8.
Balic A, Mina M. Analysis of developmental potentials of dental pulp in vitro using GFP transgenes. Orthod Craniofac Res. 2005;8(4):252-8.
Balic, A., & Mina, M. (2005). Analysis of developmental potentials of dental pulp in vitro using GFP transgenes. Orthodontics & Craniofacial Research, 8(4), 252-8.
Balic A, Mina M. Analysis of Developmental Potentials of Dental Pulp in Vitro Using GFP Transgenes. Orthod Craniofac Res. 2005;8(4):252-8. PubMed PMID: 16238605.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of developmental potentials of dental pulp in vitro using GFP transgenes. AU - Balic,A, AU - Mina,M, PY - 2005/10/22/pubmed PY - 2005/12/24/medline PY - 2005/10/22/entrez SP - 252 EP - 8 JF - Orthodontics & craniofacial research JO - Orthod Craniofac Res VL - 8 IS - 4 N2 - BACKGROUND: In recent years there has been increasing progress in identifying stem cells from adult tissues and their potential application for tooth replacement/regeneration. Our previous in vivo studies show that pOBCol3.6GFP and pOBCol2.3GFP transgenic animals provide a unique model to gain insight into progenitor/stem cells in the dental pulp capable of giving rise to odontoblasts. OBJECTIVES: To characterize the behavior of dental pulp cells derived from pOBCol3.6GFP animals in vitro. EXPERIMENTAL DESIGN: Primary cultures were established from the coronal portions of the pulps isolated first molars from 5-day-old pOBCol3.6GFP heterozygous mice and grown for 21 days. In these cultures proliferation, clonogenic capacity, activation of 3.6-GFP and mineralization were examined. RESULTS: Our observations show that dental pulp cells derived from 3.6-GFP contain a population of proliferative, clonogenic cells with the ability to mineralize. We also show the stage specific activation/upregulation of 3.6-GFP in primary cultures derived from dental pulp. In these cultures, expression of Col1a1-3.6-GFP occurs prior to the appearance of mineralized nodules and is unregulated in mineralized nodules. CONCLUSIONS: Col1a1-GFP transgenes appear to fulfill many of the requirements of a marker gene for cell lineage studies in intact tooth and primary cultures derived from dental pulp. SN - 1601-6335 UR - https://www.unboundmedicine.com/medline/citation/16238605/Analysis_of_developmental_potentials_of_dental_pulp_in_vitro_using_GFP_transgenes_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=1601-6335&date=2005&volume=8&issue=4&spage=252 DB - PRIME DP - Unbound Medicine ER -