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Distribution of follicle-stimulating hormone and luteinizing hormone isoforms in sera from women with primary ovarian failure compared with that of normal reproductive and postmenopausal women.
Fertil Steril. 1992 Jul; 58(1):60-5.FS

Abstract

OBJECTIVE

To demonstrate if molecular heterogeneity of gonadotropins correlates with the type of primary gonadal failure.

DESIGN AND METHODS

Aliquots of sera from women with hypogonadism were subjected to gel filtration chromatography to be assayed for follicle-stimulating hormone (FSH) and luteinizing hormone (LH) by the use of radioimmunoassay. Molecular weight (MW) of isoforms was calculated on a calibration curve obtained with molecular markers. The molecular variants were characterized on the basis of elution volume, MW, and partition coefficient.

RESULTS

Chromatographic profile of sera from four women with natural menopause exhibited two FSH peaks of immunoreactivity and a heavier LH isoform. This pattern was different from that obtained in sera from women of reproductive age who presented a single peak that eluted after the corresponding standard. In six cases of idiopathic premature menopause and three more with gonadotropin-resistant ovary, the chromatographic profile showed a marked and remarkable molecular heterogeneity, particularly LH, and this was more apparent in women with resistant ovary.

CONCLUSIONS

Our investigation confirms the relationship between the gonadotropin heterogeneity with the gonadal failure. The duration of the ovarian failure may influence the molecular proportion of gonadotropins and the predominance of heavier MW isohormones.

Authors+Show Affiliations

Endocrine Research Unit, Instituto Mexicano del Seguro Social, D.F.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1624024

Citation

Mason, M, et al. "Distribution of Follicle-stimulating Hormone and Luteinizing Hormone Isoforms in Sera From Women With Primary Ovarian Failure Compared With That of Normal Reproductive and Postmenopausal Women." Fertility and Sterility, vol. 58, no. 1, 1992, pp. 60-5.
Mason M, Fonseca E, Ruiz JE, et al. Distribution of follicle-stimulating hormone and luteinizing hormone isoforms in sera from women with primary ovarian failure compared with that of normal reproductive and postmenopausal women. Fertil Steril. 1992;58(1):60-5.
Mason, M., Fonseca, E., Ruiz, J. E., Morán, C., & Zárate, A. (1992). Distribution of follicle-stimulating hormone and luteinizing hormone isoforms in sera from women with primary ovarian failure compared with that of normal reproductive and postmenopausal women. Fertility and Sterility, 58(1), 60-5.
Mason M, et al. Distribution of Follicle-stimulating Hormone and Luteinizing Hormone Isoforms in Sera From Women With Primary Ovarian Failure Compared With That of Normal Reproductive and Postmenopausal Women. Fertil Steril. 1992;58(1):60-5. PubMed PMID: 1624024.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of follicle-stimulating hormone and luteinizing hormone isoforms in sera from women with primary ovarian failure compared with that of normal reproductive and postmenopausal women. AU - Mason,M, AU - Fonseca,E, AU - Ruiz,J E, AU - Morán,C, AU - Zárate,A, PY - 1992/7/1/pubmed PY - 1992/7/1/medline PY - 1992/7/1/entrez SP - 60 EP - 5 JF - Fertility and sterility JO - Fertil Steril VL - 58 IS - 1 N2 - OBJECTIVE: To demonstrate if molecular heterogeneity of gonadotropins correlates with the type of primary gonadal failure. DESIGN AND METHODS: Aliquots of sera from women with hypogonadism were subjected to gel filtration chromatography to be assayed for follicle-stimulating hormone (FSH) and luteinizing hormone (LH) by the use of radioimmunoassay. Molecular weight (MW) of isoforms was calculated on a calibration curve obtained with molecular markers. The molecular variants were characterized on the basis of elution volume, MW, and partition coefficient. RESULTS: Chromatographic profile of sera from four women with natural menopause exhibited two FSH peaks of immunoreactivity and a heavier LH isoform. This pattern was different from that obtained in sera from women of reproductive age who presented a single peak that eluted after the corresponding standard. In six cases of idiopathic premature menopause and three more with gonadotropin-resistant ovary, the chromatographic profile showed a marked and remarkable molecular heterogeneity, particularly LH, and this was more apparent in women with resistant ovary. CONCLUSIONS: Our investigation confirms the relationship between the gonadotropin heterogeneity with the gonadal failure. The duration of the ovarian failure may influence the molecular proportion of gonadotropins and the predominance of heavier MW isohormones. SN - 0015-0282 UR - https://www.unboundmedicine.com/medline/citation/1624024/Distribution_of_follicle_stimulating_hormone_and_luteinizing_hormone_isoforms_in_sera_from_women_with_primary_ovarian_failure_compared_with_that_of_normal_reproductive_and_postmenopausal_women_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(16)55137-1 DB - PRIME DP - Unbound Medicine ER -