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Molecular genetic study of introgression between Saccharomyces bayanus and S. cerevisiae.
Yeast. 2005 Oct 30; 22(14):1099-115.Y

Abstract

The genomic constitution of different S. bayanus strains and natural interspecific Saccharomyces hybrids has been studied by genetic and molecular methods. Unlike S. bayanus var. uvarum, some S. bayanus var. bayanus strains (the type culture CBS 380, CBS 378, CBS 425, CBS 1548) harbour a number of S. cerevisiae subtelomeric sequences: Y', pEL50, SUC, RTM and MAL. The two varieties, having 86-100% nDNA-nDNA reassociation, are partly genetically isolated from one another but completely isolated from S. cerevisiae. Genetic and molecular data support the maintaining of var. bayanus and var. uvarum strains in the species S. bayanus. Using Southern hybridization with species-specific molecular markers, RFLP of the MET2 gene and flow cytometry analysis, we showed that the non-S. cerevisiae parents are different in lager brewing yeasts and in wine hybrid strains. Our results suggest that S. pastorianus is a hybrid between S. cerevisiae and S. bayanus var. bayanus, while S. bayanus var. uvarum contributed to the formation of the wine hybrids S6U and CID1. According to the partial sequence of ACT1 gene and flow cytometry analysis, strain CID1 is a triple hybrid between S. cerevisiae, S. kudriavzevii and S. bayanus var. uvarum.

Authors+Show Affiliations

Naumova ES
State Institute for Genetics and Selection of Industrial Microorganisms, Moscow 117545, Russia.
Naumov GI
No affiliation info available
Masneuf-Pomarède I
No affiliation info available
Aigle M
No affiliation info available
Dubourdieu D
No affiliation info available

MeSH

BeerBeveragesBlotting, SouthernCrosses, GeneticDNA, FungalFlow CytometryFruitGenome, FungalHybridization, GeneticPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthSaccharomycesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSpecies SpecificityTelomereWine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16240458

Citation

Naumova, Elena S., et al. "Molecular Genetic Study of Introgression Between Saccharomyces Bayanus and S. Cerevisiae." Yeast (Chichester, England), vol. 22, no. 14, 2005, pp. 1099-115.
Naumova ES, Naumov GI, Masneuf-Pomarède I, et al. Molecular genetic study of introgression between Saccharomyces bayanus and S. cerevisiae. Yeast. 2005;22(14):1099-115.
Naumova, E. S., Naumov, G. I., Masneuf-Pomarède, I., Aigle, M., & Dubourdieu, D. (2005). Molecular genetic study of introgression between Saccharomyces bayanus and S. cerevisiae. Yeast (Chichester, England), 22(14), 1099-115.
Naumova ES, et al. Molecular Genetic Study of Introgression Between Saccharomyces Bayanus and S. Cerevisiae. Yeast. 2005 Oct 30;22(14):1099-115. PubMed PMID: 16240458.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular genetic study of introgression between Saccharomyces bayanus and S. cerevisiae. AU - Naumova,Elena S, AU - Naumov,Gennadi I, AU - Masneuf-Pomarède,Isabelle, AU - Aigle,Michel, AU - Dubourdieu,Denis, PY - 2005/10/22/pubmed PY - 2005/12/21/medline PY - 2005/10/22/entrez SP - 1099 EP - 115 JF - Yeast (Chichester, England) JO - Yeast VL - 22 IS - 14 N2 - The genomic constitution of different S. bayanus strains and natural interspecific Saccharomyces hybrids has been studied by genetic and molecular methods. Unlike S. bayanus var. uvarum, some S. bayanus var. bayanus strains (the type culture CBS 380, CBS 378, CBS 425, CBS 1548) harbour a number of S. cerevisiae subtelomeric sequences: Y', pEL50, SUC, RTM and MAL. The two varieties, having 86-100% nDNA-nDNA reassociation, are partly genetically isolated from one another but completely isolated from S. cerevisiae. Genetic and molecular data support the maintaining of var. bayanus and var. uvarum strains in the species S. bayanus. Using Southern hybridization with species-specific molecular markers, RFLP of the MET2 gene and flow cytometry analysis, we showed that the non-S. cerevisiae parents are different in lager brewing yeasts and in wine hybrid strains. Our results suggest that S. pastorianus is a hybrid between S. cerevisiae and S. bayanus var. bayanus, while S. bayanus var. uvarum contributed to the formation of the wine hybrids S6U and CID1. According to the partial sequence of ACT1 gene and flow cytometry analysis, strain CID1 is a triple hybrid between S. cerevisiae, S. kudriavzevii and S. bayanus var. uvarum. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/16240458/Molecular_genetic_study_of_introgression_between_Saccharomyces_bayanus_and_S__cerevisiae_ DB - PRIME DP - Unbound Medicine ER -