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Miniaturized glucose biosensor modified with a nitric oxide-releasing xerogel microarray.
Biosens Bioelectron. 2005 Nov 15; 21(5):749-57.BB

Abstract

An enzyme-based glucose biosensor modified to release nitric oxide (NO) via a xerogel microarray is reported. The biosensor design is as follows: (1) glucose oxidase (GOx) is immobilized in a methyltrimethoxysilane (MTMOS) xerogel layer; (2) a blended polyurethane/hydrophilic polyurethane coating prevents enzyme leaching and imparts selectivity for glucose; and (3) micropatterned xerogel lines (5 microm wide) separated by distances of 5 or 20 microm provide NO-release capability. This configuration allows for increased glucose sensitivity relative to sensors modified with NO-releasing xerogel films since significant portions of the sensor surface remain unmodified. Glucose diffusion to the GOx layer is thus less inhibited. The micropatterned NO-releasing biosensors generate sufficient NO levels to reduce both Pseudomonas aeruginosa and platelet adhesion without significantly compromising the enzymatic activity of GOx. The glucose response, linearity and stability of the NO-releasing micropatterned sensors are reported.

Authors+Show Affiliations

Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3290, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16242614

Citation

Oh, Bong Kyun, et al. "Miniaturized Glucose Biosensor Modified With a Nitric Oxide-releasing Xerogel Microarray." Biosensors & Bioelectronics, vol. 21, no. 5, 2005, pp. 749-57.
Oh BK, Robbins ME, Nablo BJ, et al. Miniaturized glucose biosensor modified with a nitric oxide-releasing xerogel microarray. Biosens Bioelectron. 2005;21(5):749-57.
Oh, B. K., Robbins, M. E., Nablo, B. J., & Schoenfisch, M. H. (2005). Miniaturized glucose biosensor modified with a nitric oxide-releasing xerogel microarray. Biosensors & Bioelectronics, 21(5), 749-57.
Oh BK, et al. Miniaturized Glucose Biosensor Modified With a Nitric Oxide-releasing Xerogel Microarray. Biosens Bioelectron. 2005 Nov 15;21(5):749-57. PubMed PMID: 16242614.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Miniaturized glucose biosensor modified with a nitric oxide-releasing xerogel microarray. AU - Oh,Bong Kyun, AU - Robbins,Mary E, AU - Nablo,Brian J, AU - Schoenfisch,Mark H, PY - 2004/11/01/received PY - 2004/12/21/revised PY - 2005/01/12/accepted PY - 2005/10/26/pubmed PY - 2006/2/16/medline PY - 2005/10/26/entrez SP - 749 EP - 57 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 21 IS - 5 N2 - An enzyme-based glucose biosensor modified to release nitric oxide (NO) via a xerogel microarray is reported. The biosensor design is as follows: (1) glucose oxidase (GOx) is immobilized in a methyltrimethoxysilane (MTMOS) xerogel layer; (2) a blended polyurethane/hydrophilic polyurethane coating prevents enzyme leaching and imparts selectivity for glucose; and (3) micropatterned xerogel lines (5 microm wide) separated by distances of 5 or 20 microm provide NO-release capability. This configuration allows for increased glucose sensitivity relative to sensors modified with NO-releasing xerogel films since significant portions of the sensor surface remain unmodified. Glucose diffusion to the GOx layer is thus less inhibited. The micropatterned NO-releasing biosensors generate sufficient NO levels to reduce both Pseudomonas aeruginosa and platelet adhesion without significantly compromising the enzymatic activity of GOx. The glucose response, linearity and stability of the NO-releasing micropatterned sensors are reported. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16242614/Miniaturized_glucose_biosensor_modified_with_a_nitric_oxide_releasing_xerogel_microarray_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(05)00025-4 DB - PRIME DP - Unbound Medicine ER -