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[Determination of two main components in bark of Paeonia suffruticosa by HPLC].
Zhongguo Zhong Yao Za Zhi. 2005 Aug; 30(16):1265-8.ZZ

Abstract

OBJECTIVE

To establish a HPLC method for determination two constituents in bark of Paeonia Suffuticosa.

METHOD

The reversed phase HPLC system consisting of an Alltima ODS column (4.6 mm x 150 mm, 5 microm) and a mixture of water-THF-methanol-HAc (60:20:20:0.05) as the mobile phase was used. The flow rate was 0.8 mL x min(-1) and UV detection was set at 274 nm.

RESULT

The assay displayed good linearity over the concentration ranges of 0.06-1.0 microg (r = 0.999 9, gallic acid) and 0.16-2.58 microg (r = 0.999 9, paeonol) respectively. The average recoveries (n = 9) of gallic acid and paeonol were 98.6% (RSD = 3.0%), 98.2% (RSD = 2.5%), respectively. The samples were extracted with methanol for 24 h bu maceration.

CONCLUSION

The method is simple, accurate and can be used for the quality study of bark of P. suffruticosa.

Authors+Show Affiliations

Tian YZ
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Befiing 100050, China.
Wang Y
No affiliation info available
Liu JZ
No affiliation info available
Yang BB
No affiliation info available
Shi JG
No affiliation info available
Wang MZ
No affiliation info available

MeSH

AcetophenonesChromatography, High Pressure LiquidGallic AcidPaeoniaPlant BarkPlants, MedicinalQuality Control

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16245906

Citation

Tian, Yuan-zi, et al. "[Determination of Two Main Components in Bark of Paeonia Suffruticosa By HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 30, no. 16, 2005, pp. 1265-8.
Tian YZ, Wang Y, Liu JZ, et al. [Determination of two main components in bark of Paeonia suffruticosa by HPLC]. Zhongguo Zhong Yao Za Zhi. 2005;30(16):1265-8.
Tian, Y. Z., Wang, Y., Liu, J. Z., Yang, B. B., Shi, J. G., & Wang, M. Z. (2005). [Determination of two main components in bark of Paeonia suffruticosa by HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 30(16), 1265-8.
Tian YZ, et al. [Determination of Two Main Components in Bark of Paeonia Suffruticosa By HPLC]. Zhongguo Zhong Yao Za Zhi. 2005;30(16):1265-8. PubMed PMID: 16245906.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of two main components in bark of Paeonia suffruticosa by HPLC]. AU - Tian,Yuan-zi, AU - Wang,Yan, AU - Liu,Jian-zhen, AU - Yang,Bei-bei, AU - Shi,Jian-gong, AU - Wang,Mu-zou, PY - 2005/10/26/pubmed PY - 2007/2/3/medline PY - 2005/10/26/entrez SP - 1265 EP - 8 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 30 IS - 16 N2 - OBJECTIVE: To establish a HPLC method for determination two constituents in bark of Paeonia Suffuticosa. METHOD: The reversed phase HPLC system consisting of an Alltima ODS column (4.6 mm x 150 mm, 5 microm) and a mixture of water-THF-methanol-HAc (60:20:20:0.05) as the mobile phase was used. The flow rate was 0.8 mL x min(-1) and UV detection was set at 274 nm. RESULT: The assay displayed good linearity over the concentration ranges of 0.06-1.0 microg (r = 0.999 9, gallic acid) and 0.16-2.58 microg (r = 0.999 9, paeonol) respectively. The average recoveries (n = 9) of gallic acid and paeonol were 98.6% (RSD = 3.0%), 98.2% (RSD = 2.5%), respectively. The samples were extracted with methanol for 24 h bu maceration. CONCLUSION: The method is simple, accurate and can be used for the quality study of bark of P. suffruticosa. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/16245906/[Determination_of_two_main_components_in_bark_of_Paeonia_suffruticosa_by_HPLC]_ DB - PRIME DP - Unbound Medicine ER -