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Performance of bean bruchids Callosobruchus maculatus and Zabrotes subfasciatus (Coleoptera: Bruchidae) reared on resistant (IT81D-1045) and susceptible (Epace 10) Vigna unguiculata seeds: relationship with trypsin inhibitor and vicilin excretion.
Comp Biochem Physiol A Mol Integr Physiol. 2005 Dec; 142(4):422-6.CB

Abstract

Callosobruchus maculatus (Cm) and Zabrotes subfasciatus (Zs) were reared on resistant (IT81D-1045) and on susceptible (Epace 10) cowpea seeds. The emergence of adult insects, total developmental period (TDP) and excretion of trypsin inhibitor and vicilin were determined for both bruchid populations. Parameter evaluation showed that the Zs populations emerged from both seeds had no significant differences in emergence and TDP. The Cm population raised from resistant seeds had lower emergence (5.6+/-1.3%) and delayed TDP (46+/-1.25 days) than those emerged from susceptible seeds. The excretion of defense proteins showed that Zs reared in resistant seeds excreted 1.7 times more trypsin inhibitor, but this did not affect emergence or TDP. Furthermore, Cm population emerged from resistant seeds excreted 7 times higher vicilin and 0.4 times less trypsin inhibitor than that emerged from susceptible seeds. These results indicate that vicilins from resistant seeds are involved to significantly longer TDP (46 days) and also drastic reduction of insect emergence (approximately 5%) of C. maculatus.

Authors+Show Affiliations

Sales MP
Laboratório de Química e Função de Proteínas Bioativas, Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal 59072-970, RN, Brazil. msales@cb.ufrn.br
Andrade LB
No affiliation info available
Ary MB
No affiliation info available
Miranda MR
No affiliation info available
Teixeira FM
No affiliation info available
Oliveira AS
No affiliation info available
Fernandes KV
No affiliation info available
Xavier-Filho J
No affiliation info available

MeSH

AnimalsColeopteraElectrophoresis, Polyacrylamide GelFabaceaeMolecular WeightPlant ProteinsSeed Storage ProteinsSeedsTrypsin Inhibitors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16246611

Citation

Sales, M P., et al. "Performance of Bean Bruchids Callosobruchus Maculatus and Zabrotes Subfasciatus (Coleoptera: Bruchidae) Reared On Resistant (IT81D-1045) and Susceptible (Epace 10) Vigna Unguiculata Seeds: Relationship With Trypsin Inhibitor and Vicilin Excretion." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 142, no. 4, 2005, pp. 422-6.
Sales MP, Andrade LB, Ary MB, et al. Performance of bean bruchids Callosobruchus maculatus and Zabrotes subfasciatus (Coleoptera: Bruchidae) reared on resistant (IT81D-1045) and susceptible (Epace 10) Vigna unguiculata seeds: relationship with trypsin inhibitor and vicilin excretion. Comp Biochem Physiol A Mol Integr Physiol. 2005;142(4):422-6.
Sales, M. P., Andrade, L. B., Ary, M. B., Miranda, M. R., Teixeira, F. M., Oliveira, A. S., Fernandes, K. V., & Xavier-Filho, J. (2005). Performance of bean bruchids Callosobruchus maculatus and Zabrotes subfasciatus (Coleoptera: Bruchidae) reared on resistant (IT81D-1045) and susceptible (Epace 10) Vigna unguiculata seeds: relationship with trypsin inhibitor and vicilin excretion. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 142(4), 422-6.
Sales MP, et al. Performance of Bean Bruchids Callosobruchus Maculatus and Zabrotes Subfasciatus (Coleoptera: Bruchidae) Reared On Resistant (IT81D-1045) and Susceptible (Epace 10) Vigna Unguiculata Seeds: Relationship With Trypsin Inhibitor and Vicilin Excretion. Comp Biochem Physiol A Mol Integr Physiol. 2005;142(4):422-6. PubMed PMID: 16246611.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Performance of bean bruchids Callosobruchus maculatus and Zabrotes subfasciatus (Coleoptera: Bruchidae) reared on resistant (IT81D-1045) and susceptible (Epace 10) Vigna unguiculata seeds: relationship with trypsin inhibitor and vicilin excretion. AU - Sales,M P, AU - Andrade,L B S, AU - Ary,M B, AU - Miranda,M R A, AU - Teixeira,F M, AU - Oliveira,A S, AU - Fernandes,K V S, AU - Xavier-Filho,J, Y1 - 2005/10/24/ PY - 2005/02/13/received PY - 2005/09/02/revised PY - 2005/09/10/accepted PY - 2005/10/26/pubmed PY - 2006/3/18/medline PY - 2005/10/26/entrez SP - 422 EP - 6 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp Biochem Physiol A Mol Integr Physiol VL - 142 IS - 4 N2 - Callosobruchus maculatus (Cm) and Zabrotes subfasciatus (Zs) were reared on resistant (IT81D-1045) and on susceptible (Epace 10) cowpea seeds. The emergence of adult insects, total developmental period (TDP) and excretion of trypsin inhibitor and vicilin were determined for both bruchid populations. Parameter evaluation showed that the Zs populations emerged from both seeds had no significant differences in emergence and TDP. The Cm population raised from resistant seeds had lower emergence (5.6+/-1.3%) and delayed TDP (46+/-1.25 days) than those emerged from susceptible seeds. The excretion of defense proteins showed that Zs reared in resistant seeds excreted 1.7 times more trypsin inhibitor, but this did not affect emergence or TDP. Furthermore, Cm population emerged from resistant seeds excreted 7 times higher vicilin and 0.4 times less trypsin inhibitor than that emerged from susceptible seeds. These results indicate that vicilins from resistant seeds are involved to significantly longer TDP (46 days) and also drastic reduction of insect emergence (approximately 5%) of C. maculatus. SN - 1095-6433 UR - https://www.unboundmedicine.com/medline/citation/16246611/Performance_of_bean_bruchids_Callosobruchus_maculatus_and_Zabrotes_subfasciatus__Coleoptera:_Bruchidae__reared_on_resistant__IT81D_1045__and_susceptible__Epace_10__Vigna_unguiculata_seeds:_relationship_with_trypsin_inhibitor_and_vicilin_excretion_ DB - PRIME DP - Unbound Medicine ER -

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