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Simultaneous determination of multiple phytohormones in plant extracts by liquid chromatography-electrospray tandem mass spectrometry.
J Agric Food Chem. 2005 Nov 02; 53(22):8437-42.JA

Abstract

A rapid multiresidue method to quantify three different classes of plant hormones has been developed. The reduced concentrations of these metabolites in real samples with complex matrixes require sensitive techniques for their quantification in small amounts of plant tissue. The method described combines high-performance liquid chromatography with electrospray ionization tandem mass spectrometry. Deuterium-labeled standards were added prior to sample extraction to achieve an accurate quantification of abscisic acid, indole-3-acetic acid, and jasmonic acid in a single run. A simple method of extraction and purification involving only centrifugation, a partition against diethyl ether, and filtration was developed and the analytical method validated in four different plant tissues, citrus leaves, papaya roots, barley seedlings, and barley immature embryos. This method represents a clear advantage because it extensively reduces sample preparation and total time for routine analysis of phytohormones in real plant samples.

Authors+Show Affiliations

Departamento de Ciencias Experimentales, Universitat Jaume I, Campus Riu Sec, E-12071 Castelló, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16248534

Citation

Durgbanshi, Abhilasha, et al. "Simultaneous Determination of Multiple Phytohormones in Plant Extracts By Liquid Chromatography-electrospray Tandem Mass Spectrometry." Journal of Agricultural and Food Chemistry, vol. 53, no. 22, 2005, pp. 8437-42.
Durgbanshi A, Arbona V, Pozo O, et al. Simultaneous determination of multiple phytohormones in plant extracts by liquid chromatography-electrospray tandem mass spectrometry. J Agric Food Chem. 2005;53(22):8437-42.
Durgbanshi, A., Arbona, V., Pozo, O., Miersch, O., Sancho, J. V., & Gómez-Cadenas, A. (2005). Simultaneous determination of multiple phytohormones in plant extracts by liquid chromatography-electrospray tandem mass spectrometry. Journal of Agricultural and Food Chemistry, 53(22), 8437-42.
Durgbanshi A, et al. Simultaneous Determination of Multiple Phytohormones in Plant Extracts By Liquid Chromatography-electrospray Tandem Mass Spectrometry. J Agric Food Chem. 2005 Nov 2;53(22):8437-42. PubMed PMID: 16248534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of multiple phytohormones in plant extracts by liquid chromatography-electrospray tandem mass spectrometry. AU - Durgbanshi,Abhilasha, AU - Arbona,Vicent, AU - Pozo,Oscar, AU - Miersch,Otto, AU - Sancho,Juan V, AU - Gómez-Cadenas,Aurelio, PY - 2005/10/27/pubmed PY - 2005/12/24/medline PY - 2005/10/27/entrez SP - 8437 EP - 42 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 53 IS - 22 N2 - A rapid multiresidue method to quantify three different classes of plant hormones has been developed. The reduced concentrations of these metabolites in real samples with complex matrixes require sensitive techniques for their quantification in small amounts of plant tissue. The method described combines high-performance liquid chromatography with electrospray ionization tandem mass spectrometry. Deuterium-labeled standards were added prior to sample extraction to achieve an accurate quantification of abscisic acid, indole-3-acetic acid, and jasmonic acid in a single run. A simple method of extraction and purification involving only centrifugation, a partition against diethyl ether, and filtration was developed and the analytical method validated in four different plant tissues, citrus leaves, papaya roots, barley seedlings, and barley immature embryos. This method represents a clear advantage because it extensively reduces sample preparation and total time for routine analysis of phytohormones in real plant samples. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/16248534/Simultaneous_determination_of_multiple_phytohormones_in_plant_extracts_by_liquid_chromatography_electrospray_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -