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Study of anticancer activities of muscadine grape phenolics in vitro.
J Agric Food Chem. 2005 Nov 02; 53(22):8804-12.JA

Abstract

Muscadine grapes have unique aroma and flavor characteristics. Although a few studies reported high polyphenols content of muscadine grapes, little research has been conducted to evaluate the phenolic compounds bioactivities in any muscadine grape cultivar. The objective of this study was to evaluate the effect of phenolic compounds in muscadine grapes on cancer cell viability and apoptosis. Four cultivars of muscadine (Carlos, Ison, Noble, and Supreme) were assessed in this study. Phenolic compounds were extracted from muscadine skins and further separated into phenolic acids, tannins, flavonols, and anthocyanins using HLB cartridge and LH20 column. Some individual phenolic acids and flavonoids were identified by HPLC. Anthocyanin fractions were more than 90% pure. The effect of different fractions on the viability and apoptosis of two colon cancer cell lines (HT-29 and Caco-2) was evaluated. A 50% inhibition of cancer cell population growth for the two cell lines was observed at concentrations of 1-7 mg/mL for crude extracts. The phenolic acid fractions showed a 50% inhibition at the level of 0.5-3 mg/mL. The greatest inhibitory activity was found in the anthocyanin fraction, with a 50% inhibition at concentrations of approximately 200 microg/mL in HT-29 and 100-300 microg/mL in Caco-2. Anthocyanin fractions also resulted in 2-4 times increase in DNA fragmentation, indicating the induction of apoptosis. These findings suggest that polyphenols from muscadine grapes may have anticancer properties.

Authors+Show Affiliations

Department of Food Science and Technology, The University of Georgia, Athens, GA 30602-7610, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16248588

Citation

Yi, Weiguang, et al. "Study of Anticancer Activities of Muscadine Grape Phenolics in Vitro." Journal of Agricultural and Food Chemistry, vol. 53, no. 22, 2005, pp. 8804-12.
Yi W, Fischer J, Akoh CC. Study of anticancer activities of muscadine grape phenolics in vitro. J Agric Food Chem. 2005;53(22):8804-12.
Yi, W., Fischer, J., & Akoh, C. C. (2005). Study of anticancer activities of muscadine grape phenolics in vitro. Journal of Agricultural and Food Chemistry, 53(22), 8804-12.
Yi W, Fischer J, Akoh CC. Study of Anticancer Activities of Muscadine Grape Phenolics in Vitro. J Agric Food Chem. 2005 Nov 2;53(22):8804-12. PubMed PMID: 16248588.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Study of anticancer activities of muscadine grape phenolics in vitro. AU - Yi,Weiguang, AU - Fischer,Joan, AU - Akoh,Casimir C, PY - 2005/10/27/pubmed PY - 2005/12/24/medline PY - 2005/10/27/entrez SP - 8804 EP - 12 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 53 IS - 22 N2 - Muscadine grapes have unique aroma and flavor characteristics. Although a few studies reported high polyphenols content of muscadine grapes, little research has been conducted to evaluate the phenolic compounds bioactivities in any muscadine grape cultivar. The objective of this study was to evaluate the effect of phenolic compounds in muscadine grapes on cancer cell viability and apoptosis. Four cultivars of muscadine (Carlos, Ison, Noble, and Supreme) were assessed in this study. Phenolic compounds were extracted from muscadine skins and further separated into phenolic acids, tannins, flavonols, and anthocyanins using HLB cartridge and LH20 column. Some individual phenolic acids and flavonoids were identified by HPLC. Anthocyanin fractions were more than 90% pure. The effect of different fractions on the viability and apoptosis of two colon cancer cell lines (HT-29 and Caco-2) was evaluated. A 50% inhibition of cancer cell population growth for the two cell lines was observed at concentrations of 1-7 mg/mL for crude extracts. The phenolic acid fractions showed a 50% inhibition at the level of 0.5-3 mg/mL. The greatest inhibitory activity was found in the anthocyanin fraction, with a 50% inhibition at concentrations of approximately 200 microg/mL in HT-29 and 100-300 microg/mL in Caco-2. Anthocyanin fractions also resulted in 2-4 times increase in DNA fragmentation, indicating the induction of apoptosis. These findings suggest that polyphenols from muscadine grapes may have anticancer properties. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/16248588/Study_of_anticancer_activities_of_muscadine_grape_phenolics_in_vitro_ L2 - https://doi.org/10.1021/jf0515328 DB - PRIME DP - Unbound Medicine ER -