Tags

Type your tag names separated by a space and hit enter

Antioxidant effect of ascorbic acid on PCB (Aroclor 1254) induced oxidative stress in hypothalamus of albino rats.
Clin Chim Acta. 2006 Mar; 365(1-2):297-303.CC

Abstract

BACKGROUND

PCBs are one of the environmental toxicants and neurotoxic compounds which induce the production of free radicals leading to oxidative stress. Vitamin C is well known as an outstanding antioxidant. We determined the protective role of ascorbate on hypothalamic antioxidant system of Aroclor 1254 exposed rats.

METHODS

The rats were injected Aroclor 1254 at a dose of 2 mg/kg bw/day intraperitoneally for 30 days. One group of rats received vitamin C (100 mg/kg bw/day) orally simultaneously with Aroclor 1254 for 30 days. Twenty-four hours after last treatment, the animals were killed and hypothalamic region was separated from brain tissue. Enzymatic and non-enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and vitamin C were estimated. Hydrogen peroxide (H(2)O(2)), lipid peroxidation (LPO) and acetylcholine esterase (AchE) activity were determined. Serum gonadotropins such as luteinizing hormone (LH) and follicle stimulating hormone (FSH) were also assayed.

RESULTS

Activities of SOD, CAT, GPx, GR, AchE and the concentration of vitamin C were decreased while an increase in H(2)O(2) and LPO were observed in hypothalamus of PCB treated animals. LH and FSH concentrations were also decreased in serum of PCB exposed animals. Vitamin C administration retrieved all the parameters significantly except serum hormonal profiles.

CONCLUSION

PCB induces oxidative stress in hypothalamus by decreasing the activities of antioxidant enzymes, which can be protected by vitamin C treatment.

Authors+Show Affiliations

Department of Endocrinology, University of Madras, Taramani campus, Chennai 600113, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16248992

Citation

Muthuvel, R, et al. "Antioxidant Effect of Ascorbic Acid On PCB (Aroclor 1254) Induced Oxidative Stress in Hypothalamus of Albino Rats." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 365, no. 1-2, 2006, pp. 297-303.
Muthuvel R, Venkataraman P, Krishnamoorthy G, et al. Antioxidant effect of ascorbic acid on PCB (Aroclor 1254) induced oxidative stress in hypothalamus of albino rats. Clin Chim Acta. 2006;365(1-2):297-303.
Muthuvel, R., Venkataraman, P., Krishnamoorthy, G., Gunadharini, D. N., Kanagaraj, P., Jone Stanley, A., Srinivasan, N., Balasubramanian, K., Aruldhas, M. M., & Arunakaran, J. (2006). Antioxidant effect of ascorbic acid on PCB (Aroclor 1254) induced oxidative stress in hypothalamus of albino rats. Clinica Chimica Acta; International Journal of Clinical Chemistry, 365(1-2), 297-303.
Muthuvel R, et al. Antioxidant Effect of Ascorbic Acid On PCB (Aroclor 1254) Induced Oxidative Stress in Hypothalamus of Albino Rats. Clin Chim Acta. 2006;365(1-2):297-303. PubMed PMID: 16248992.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant effect of ascorbic acid on PCB (Aroclor 1254) induced oxidative stress in hypothalamus of albino rats. AU - Muthuvel,R, AU - Venkataraman,P, AU - Krishnamoorthy,G, AU - Gunadharini,D N, AU - Kanagaraj,P, AU - Jone Stanley,A, AU - Srinivasan,N, AU - Balasubramanian,K, AU - Aruldhas,M M, AU - Arunakaran,J, Y1 - 2005/10/24/ PY - 2005/06/30/received PY - 2005/09/03/revised PY - 2005/09/08/accepted PY - 2005/10/27/pubmed PY - 2006/6/14/medline PY - 2005/10/27/entrez SP - 297 EP - 303 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin Chim Acta VL - 365 IS - 1-2 N2 - BACKGROUND: PCBs are one of the environmental toxicants and neurotoxic compounds which induce the production of free radicals leading to oxidative stress. Vitamin C is well known as an outstanding antioxidant. We determined the protective role of ascorbate on hypothalamic antioxidant system of Aroclor 1254 exposed rats. METHODS: The rats were injected Aroclor 1254 at a dose of 2 mg/kg bw/day intraperitoneally for 30 days. One group of rats received vitamin C (100 mg/kg bw/day) orally simultaneously with Aroclor 1254 for 30 days. Twenty-four hours after last treatment, the animals were killed and hypothalamic region was separated from brain tissue. Enzymatic and non-enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and vitamin C were estimated. Hydrogen peroxide (H(2)O(2)), lipid peroxidation (LPO) and acetylcholine esterase (AchE) activity were determined. Serum gonadotropins such as luteinizing hormone (LH) and follicle stimulating hormone (FSH) were also assayed. RESULTS: Activities of SOD, CAT, GPx, GR, AchE and the concentration of vitamin C were decreased while an increase in H(2)O(2) and LPO were observed in hypothalamus of PCB treated animals. LH and FSH concentrations were also decreased in serum of PCB exposed animals. Vitamin C administration retrieved all the parameters significantly except serum hormonal profiles. CONCLUSION: PCB induces oxidative stress in hypothalamus by decreasing the activities of antioxidant enzymes, which can be protected by vitamin C treatment. SN - 0009-8981 UR - https://www.unboundmedicine.com/medline/citation/16248992/Antioxidant_effect_of_ascorbic_acid_on_PCB__Aroclor_1254__induced_oxidative_stress_in_hypothalamus_of_albino_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-8981(05)00555-3 DB - PRIME DP - Unbound Medicine ER -