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Differentiating copper and arsenic toxicity using biochemical biomarkers in Asellus aquaticus and Dreissena polymorpha.
Ecotoxicol Environ Saf. 2006 Nov; 65(3):342-9.EE

Abstract

Biomarkers of metal exposure are well known, but how a suite of such biomarkers will respond if the metal is also an oxidizing agent or causes oxidative stress is unclear. This study compares the effects of copper and arsenic, two metals with different oxidizing potential, on freshwater invertebrates. Dreissena polymorpha and Asellus aquaticus were exposed to nominal concentrations of copper (100 microg/L) or arsenic (80 microg/L) over 7 days, and physiological stress was examined by measuring metallothionein (MT) induction, thiobarbituric acid reactive substances (TBARS), and Na(+)/K(+)-ATPase activity. Both species showed increased levels of MT during 7-day Cu exposure tests and transient changes in lipid peroxidation (TBARS) which decreased to control levels by day 7. Arsenic had no effect on TBARS and only a transitory effect on MT in D. polymorpha over 7 days, although it initially induced lipid peroxidation in A. aquaticus on day 3. No inhibition of the Na(+)/K(+)-ATPase enzyme was observed for exposed organisms, and baseline values reported here, for A. aquaticus, 1.1 micromol Pi/mg/h, and for D. polymorpha, 0.38 micromol Pi/mg/h, are probably the first reported for these species.

Authors+Show Affiliations

Plymouth Environmental Research Centre and School of Biological Sciences, University of Plymouth, Drakes Circus, Plymouth PL4 8AA, UK. nbouskill@plymouth.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16253326

Citation

Bouskill, Nicolas J., et al. "Differentiating Copper and Arsenic Toxicity Using Biochemical Biomarkers in Asellus Aquaticus and Dreissena Polymorpha." Ecotoxicology and Environmental Safety, vol. 65, no. 3, 2006, pp. 342-9.
Bouskill NJ, Handy RD, Ford TE, et al. Differentiating copper and arsenic toxicity using biochemical biomarkers in Asellus aquaticus and Dreissena polymorpha. Ecotoxicol Environ Saf. 2006;65(3):342-9.
Bouskill, N. J., Handy, R. D., Ford, T. E., & Galloway, T. S. (2006). Differentiating copper and arsenic toxicity using biochemical biomarkers in Asellus aquaticus and Dreissena polymorpha. Ecotoxicology and Environmental Safety, 65(3), 342-9.
Bouskill NJ, et al. Differentiating Copper and Arsenic Toxicity Using Biochemical Biomarkers in Asellus Aquaticus and Dreissena Polymorpha. Ecotoxicol Environ Saf. 2006;65(3):342-9. PubMed PMID: 16253326.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differentiating copper and arsenic toxicity using biochemical biomarkers in Asellus aquaticus and Dreissena polymorpha. AU - Bouskill,Nicolas J, AU - Handy,Richard D, AU - Ford,Tim E, AU - Galloway,Tamara S, Y1 - 2005/10/25/ PY - 2005/03/11/received PY - 2005/07/27/revised PY - 2005/07/31/accepted PY - 2005/10/29/pubmed PY - 2007/2/3/medline PY - 2005/10/29/entrez SP - 342 EP - 9 JF - Ecotoxicology and environmental safety JO - Ecotoxicol. Environ. Saf. VL - 65 IS - 3 N2 - Biomarkers of metal exposure are well known, but how a suite of such biomarkers will respond if the metal is also an oxidizing agent or causes oxidative stress is unclear. This study compares the effects of copper and arsenic, two metals with different oxidizing potential, on freshwater invertebrates. Dreissena polymorpha and Asellus aquaticus were exposed to nominal concentrations of copper (100 microg/L) or arsenic (80 microg/L) over 7 days, and physiological stress was examined by measuring metallothionein (MT) induction, thiobarbituric acid reactive substances (TBARS), and Na(+)/K(+)-ATPase activity. Both species showed increased levels of MT during 7-day Cu exposure tests and transient changes in lipid peroxidation (TBARS) which decreased to control levels by day 7. Arsenic had no effect on TBARS and only a transitory effect on MT in D. polymorpha over 7 days, although it initially induced lipid peroxidation in A. aquaticus on day 3. No inhibition of the Na(+)/K(+)-ATPase enzyme was observed for exposed organisms, and baseline values reported here, for A. aquaticus, 1.1 micromol Pi/mg/h, and for D. polymorpha, 0.38 micromol Pi/mg/h, are probably the first reported for these species. SN - 0147-6513 UR - https://www.unboundmedicine.com/medline/citation/16253326/Differentiating_copper_and_arsenic_toxicity_using_biochemical_biomarkers_in_Asellus_aquaticus_and_Dreissena_polymorpha_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(05)00223-X DB - PRIME DP - Unbound Medicine ER -