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Determination of paeonol and paeoniflorin in Chinese medicine Cortex Moutan and 'Shuangdan' granule by micellar electrokinetic capillary chromatography.
J Pharm Biomed Anal. 2006 Mar 18; 40(5):1257-62.JP

Abstract

An easy, simple and rapid micellar electrokinetic capillary chromatography (MEKC) method was developed for the separation of two active components paeonol (PN) and paeoniflorin (PF) within 7 min. Capillary electrophoresis (CE) was performed using a 50.0 cm (42.0 cm to the detector window) x 75 microm i.d. fused-silica capillary. The optimal running buffer containing 10mM borate and 25 mM SDS at pH 9.54 was employed. The applied voltage 15 kV and the temperature 25 degrees C was used in CE separation. The linearities between peak areas and the concentrations of the analytes were investigated, and they exhibited excellent linear behavior over the investigated concentration ranges (R(2): 0.9945 for PN and 0.9992 for PF). The method was successfully applied to the determination of these two components contained in Cortex Moutan and 'Shuangdan' granule. The average recoveries ranged between 97.6 and 105.3% for PN and 95.3 and 106.1% for PF, respectively.

Authors+Show Affiliations

Yu K
Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027, China.
Wang YW
No affiliation info available
Cheng YY
No affiliation info available

MeSH

AcetophenonesBenzoatesBridged-Ring CompoundsBuffersCalibrationChromatography, Micellar Electrokinetic CapillaryDrugs, Chinese HerbalGlucosidesHydrogen-Ion ConcentrationIndicators and ReagentsMonoterpenesPaeoniaReference StandardsReproducibility of ResultsSodium Dodecyl Sulfate

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16253465

Citation

Yu, Ke, et al. "Determination of Paeonol and Paeoniflorin in Chinese Medicine Cortex Moutan and 'Shuangdan' Granule By Micellar Electrokinetic Capillary Chromatography." Journal of Pharmaceutical and Biomedical Analysis, vol. 40, no. 5, 2006, pp. 1257-62.
Yu K, Wang YW, Cheng YY. Determination of paeonol and paeoniflorin in Chinese medicine Cortex Moutan and 'Shuangdan' granule by micellar electrokinetic capillary chromatography. J Pharm Biomed Anal. 2006;40(5):1257-62.
Yu, K., Wang, Y. W., & Cheng, Y. Y. (2006). Determination of paeonol and paeoniflorin in Chinese medicine Cortex Moutan and 'Shuangdan' granule by micellar electrokinetic capillary chromatography. Journal of Pharmaceutical and Biomedical Analysis, 40(5), 1257-62.
Yu K, Wang YW, Cheng YY. Determination of Paeonol and Paeoniflorin in Chinese Medicine Cortex Moutan and 'Shuangdan' Granule By Micellar Electrokinetic Capillary Chromatography. J Pharm Biomed Anal. 2006 Mar 18;40(5):1257-62. PubMed PMID: 16253465.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of paeonol and paeoniflorin in Chinese medicine Cortex Moutan and 'Shuangdan' granule by micellar electrokinetic capillary chromatography. AU - Yu,Ke, AU - Wang,Yu-Wen, AU - Cheng,Yi-Yu, Y1 - 2005/10/25/ PY - 2005/07/23/received PY - 2005/09/06/revised PY - 2005/09/08/accepted PY - 2005/10/29/pubmed PY - 2006/6/6/medline PY - 2005/10/29/entrez SP - 1257 EP - 62 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 40 IS - 5 N2 - An easy, simple and rapid micellar electrokinetic capillary chromatography (MEKC) method was developed for the separation of two active components paeonol (PN) and paeoniflorin (PF) within 7 min. Capillary electrophoresis (CE) was performed using a 50.0 cm (42.0 cm to the detector window) x 75 microm i.d. fused-silica capillary. The optimal running buffer containing 10mM borate and 25 mM SDS at pH 9.54 was employed. The applied voltage 15 kV and the temperature 25 degrees C was used in CE separation. The linearities between peak areas and the concentrations of the analytes were investigated, and they exhibited excellent linear behavior over the investigated concentration ranges (R(2): 0.9945 for PN and 0.9992 for PF). The method was successfully applied to the determination of these two components contained in Cortex Moutan and 'Shuangdan' granule. The average recoveries ranged between 97.6 and 105.3% for PN and 95.3 and 106.1% for PF, respectively. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/16253465/Determination_of_paeonol_and_paeoniflorin_in_Chinese_medicine_Cortex_Moutan_and_'Shuangdan'_granule_by_micellar_electrokinetic_capillary_chromatography_ DB - PRIME DP - Unbound Medicine ER -