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[Expression of SARS coronavirus nucleocapsid protein and construction of its DNA vaccine].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005 Nov; 21(6):731-3, 737.XB

Abstract

AIM

To express the nucleocapsid (N) protein of SARS coronavirus (SARS-CoV) in E. coli and construct its DNA vaccine.

METHODS

The prokaryotic expression vector pQEN containing N gene was constructed and transformed into the E. coli. The recombinant N protein was then expressed and purified by Ni(2+)-NTA affinity resin. In addition, the N gene was cloned into the eukaryotic expression plasmid pSecTagB and the eukaryotic recombinant expression vector pSecN was obtained. The DNA vaccine pSecN was injected to immunize the BALB/c mice to produce the antiserum against N protein of SARS-CoV. Subsequently, the reactivity of the antiserum with recombinant N protein and SARS-CoV particles was assayed by ELISA.

RESULTS

Recombinant N protein reacted strongly and specifically with the sera from immunized mice and SARS patients. Similarly, the sera of immunized mice could also react specifically with SARS-CoV particles.

CONCLUSION

The recombinant N protein could be used as a good antigen to detect SARS. The DNA vaccine pSecN could also efficiently induce the production of IgG against N protein of SARS-CoV, which offered clues to the development of a potential DNA vaccine.

Authors+Show Affiliations

Guangzhou Huayin Genetic Science and Technology Co., Ltd., Guangzhou 510150, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

16256037

Citation

Xiang, Kai-jun, et al. "[Expression of SARS Coronavirus Nucleocapsid Protein and Construction of Its DNA Vaccine]." Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, vol. 21, no. 6, 2005, pp. 731-3, 737.
Xiang KJ, Zeng QY, Ding YQ, et al. [Expression of SARS coronavirus nucleocapsid protein and construction of its DNA vaccine]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005;21(6):731-3, 737.
Xiang, K. J., Zeng, Q. Y., Ding, Y. Q., Zhu, B., & Zhou, R. (2005). [Expression of SARS coronavirus nucleocapsid protein and construction of its DNA vaccine]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, 21(6), 731-3, 737.
Xiang KJ, et al. [Expression of SARS Coronavirus Nucleocapsid Protein and Construction of Its DNA Vaccine]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005;21(6):731-3, 737. PubMed PMID: 16256037.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression of SARS coronavirus nucleocapsid protein and construction of its DNA vaccine]. AU - Xiang,Kai-jun, AU - Zeng,Qi-yi, AU - Ding,Yong-qiang, AU - Zhu,Bing, AU - Zhou,Rong, PY - 2005/11/1/pubmed PY - 2009/4/25/medline PY - 2005/11/1/entrez SP - 731-3, 737 JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology JO - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi VL - 21 IS - 6 N2 - AIM: To express the nucleocapsid (N) protein of SARS coronavirus (SARS-CoV) in E. coli and construct its DNA vaccine. METHODS: The prokaryotic expression vector pQEN containing N gene was constructed and transformed into the E. coli. The recombinant N protein was then expressed and purified by Ni(2+)-NTA affinity resin. In addition, the N gene was cloned into the eukaryotic expression plasmid pSecTagB and the eukaryotic recombinant expression vector pSecN was obtained. The DNA vaccine pSecN was injected to immunize the BALB/c mice to produce the antiserum against N protein of SARS-CoV. Subsequently, the reactivity of the antiserum with recombinant N protein and SARS-CoV particles was assayed by ELISA. RESULTS: Recombinant N protein reacted strongly and specifically with the sera from immunized mice and SARS patients. Similarly, the sera of immunized mice could also react specifically with SARS-CoV particles. CONCLUSION: The recombinant N protein could be used as a good antigen to detect SARS. The DNA vaccine pSecN could also efficiently induce the production of IgG against N protein of SARS-CoV, which offered clues to the development of a potential DNA vaccine. SN - 1007-8738 UR - https://www.unboundmedicine.com/medline/citation/16256037/[Expression_of_SARS_coronavirus_nucleocapsid_protein_and_construction_of_its_DNA_vaccine]_ DB - PRIME DP - Unbound Medicine ER -