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B7-H4 overexpression in ovarian tumors.
Gynecol Oncol. 2006 Jan; 100(1):44-52.GO

Abstract

OBJECTIVES

Despite great advances in therapeutic management, the mortality rate for ovarian cancer has remained relatively stable over the past 50 years. This study was designed to evaluate the expression of B7-H4 protein, recently identified as a potential molecular marker of breast and ovarian cancer by quantitative PCR analysis, in benign tumors, tumors of low malignant potential and malignant tumors of the ovary.

METHODS

Archival formalin-fixed tissue blocks from serous, mucinous, endometrioid and clear cell ovarian tumors were evaluated by immunohistochemistry for the distribution of B7-H4 expression, and staining intensity was measured by automated image analysis. Univariate analyses were used to test for statistically significant relationships.

RESULTS

B7-H4 cytoplasmic and membranous expression was detected in all primary serous (n = 32), endometrioid (n = 12), and clear cell carcinomas (n = 15), and in all metastatic serous (n = 23) and endometrioid (n = 7) ovarian carcinomas. By contrast, focal B7-H4 expression was detected in only 1/11 mucinous carcinomas. The proportion of positive cells and median staining intensity was greater in serous carcinomas than in serous cystadenomas or serous tumors of low malignant potential, and the differences were statistically significant (P < 0.0001 and P = 0.034, respectively). The median staining intensity was also significantly greater in endometrioid carcinomas than in endometriosis (P = 0.005).

CONCLUSIONS

The consistent overexpression of B7-H4 in serous, endometrioid and clear cell ovarian carcinomas and the relative absence of expression in most normal somatic tissues indicates that B7-H4 should be further investigated as a potential diagnostic marker or therapeutic target for ovarian cancer.

Authors+Show Affiliations

Department of Pathology, University of Colorado Health Sciences Center, B-216, 4200 East Ninth Avenue, Denver, CO 80262, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16256178

Citation

Tringler, Barbara, et al. "B7-H4 Overexpression in Ovarian Tumors." Gynecologic Oncology, vol. 100, no. 1, 2006, pp. 44-52.
Tringler B, Liu W, Corral L, et al. B7-H4 overexpression in ovarian tumors. Gynecol Oncol. 2006;100(1):44-52.
Tringler, B., Liu, W., Corral, L., Torkko, K. C., Enomoto, T., Davidson, S., Lucia, M. S., Heinz, D. E., Papkoff, J., & Shroyer, K. R. (2006). B7-H4 overexpression in ovarian tumors. Gynecologic Oncology, 100(1), 44-52.
Tringler B, et al. B7-H4 Overexpression in Ovarian Tumors. Gynecol Oncol. 2006;100(1):44-52. PubMed PMID: 16256178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - B7-H4 overexpression in ovarian tumors. AU - Tringler,Barbara, AU - Liu,Wenhui, AU - Corral,Laura, AU - Torkko,Kathleen C, AU - Enomoto,Takayuki, AU - Davidson,Susan, AU - Lucia,M Scott, AU - Heinz,David E, AU - Papkoff,Jackie, AU - Shroyer,Kenneth R, Y1 - 2005/10/26/ PY - 2005/03/25/received PY - 2005/08/06/revised PY - 2005/08/17/accepted PY - 2005/11/1/pubmed PY - 2006/2/10/medline PY - 2005/11/1/entrez SP - 44 EP - 52 JF - Gynecologic oncology JO - Gynecol Oncol VL - 100 IS - 1 N2 - OBJECTIVES: Despite great advances in therapeutic management, the mortality rate for ovarian cancer has remained relatively stable over the past 50 years. This study was designed to evaluate the expression of B7-H4 protein, recently identified as a potential molecular marker of breast and ovarian cancer by quantitative PCR analysis, in benign tumors, tumors of low malignant potential and malignant tumors of the ovary. METHODS: Archival formalin-fixed tissue blocks from serous, mucinous, endometrioid and clear cell ovarian tumors were evaluated by immunohistochemistry for the distribution of B7-H4 expression, and staining intensity was measured by automated image analysis. Univariate analyses were used to test for statistically significant relationships. RESULTS: B7-H4 cytoplasmic and membranous expression was detected in all primary serous (n = 32), endometrioid (n = 12), and clear cell carcinomas (n = 15), and in all metastatic serous (n = 23) and endometrioid (n = 7) ovarian carcinomas. By contrast, focal B7-H4 expression was detected in only 1/11 mucinous carcinomas. The proportion of positive cells and median staining intensity was greater in serous carcinomas than in serous cystadenomas or serous tumors of low malignant potential, and the differences were statistically significant (P < 0.0001 and P = 0.034, respectively). The median staining intensity was also significantly greater in endometrioid carcinomas than in endometriosis (P = 0.005). CONCLUSIONS: The consistent overexpression of B7-H4 in serous, endometrioid and clear cell ovarian carcinomas and the relative absence of expression in most normal somatic tissues indicates that B7-H4 should be further investigated as a potential diagnostic marker or therapeutic target for ovarian cancer. SN - 0090-8258 UR - https://www.unboundmedicine.com/medline/citation/16256178/B7_H4_overexpression_in_ovarian_tumors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0090-8258(05)00715-8 DB - PRIME DP - Unbound Medicine ER -