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A novel electrochemical detection method for aptamer biosensors.
Biosens Bioelectron. 2005 Dec 15; 21(6):863-70.BB

Abstract

A beacon aptamer-based biosensor for the detection of thrombin was developed using electrochemical transduction method. Gold surface was modified with a beacon aptamer covalently linked at 5'-terminus with a linker containing a primary aliphatic amine. Methylene blue (MB) was intercalated into the beacon sequence, and used as an electrochemical marker. When the beacon aptamer immobilized on gold surface encounters thrombin, the hairpin forming beacon aptamer is conformationally changed to release the intercalated MB, resulting a decrease in electrical current intensity in voltamogram. The peak signal of the MB is clearly decreased by the binding of thrombin onto the beacon aptamer. The linear range of the signal was observed between 0 and 50.8 nM of thrombin with 0.999 correlation factor. This method was able to linearly and selectively detect thrombin with a detection limit of 11 nM.

Authors+Show Affiliations

Bang GS
School of Chemical and Biological Engineering and Institute of Molecular Biology and Genetics, Seoul National University, Kwanak-ku, South Korea.
Cho S
No affiliation info available
Kim BG
No affiliation info available

MeSH

Aptamers, NucleotideBiosensing TechniquesCoated Materials, BiocompatibleElectrochemistryElectrodesEquipment DesignEquipment Failure AnalysisFeasibility StudiesMolecular Probe TechniquesThrombin

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16257654

Citation

Bang, Gyeong Sook, et al. "A Novel Electrochemical Detection Method for Aptamer Biosensors." Biosensors & Bioelectronics, vol. 21, no. 6, 2005, pp. 863-70.
Bang GS, Cho S, Kim BG. A novel electrochemical detection method for aptamer biosensors. Biosens Bioelectron. 2005;21(6):863-70.
Bang, G. S., Cho, S., & Kim, B. G. (2005). A novel electrochemical detection method for aptamer biosensors. Biosensors & Bioelectronics, 21(6), 863-70.
Bang GS, Cho S, Kim BG. A Novel Electrochemical Detection Method for Aptamer Biosensors. Biosens Bioelectron. 2005 Dec 15;21(6):863-70. PubMed PMID: 16257654.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel electrochemical detection method for aptamer biosensors. AU - Bang,Gyeong Sook, AU - Cho,Suhyeong, AU - Kim,Byung-Gee, PY - 2004/11/11/received PY - 2005/02/02/revised PY - 2005/02/04/accepted PY - 2005/11/1/pubmed PY - 2006/3/10/medline PY - 2005/11/1/entrez SP - 863 EP - 70 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 21 IS - 6 N2 - A beacon aptamer-based biosensor for the detection of thrombin was developed using electrochemical transduction method. Gold surface was modified with a beacon aptamer covalently linked at 5'-terminus with a linker containing a primary aliphatic amine. Methylene blue (MB) was intercalated into the beacon sequence, and used as an electrochemical marker. When the beacon aptamer immobilized on gold surface encounters thrombin, the hairpin forming beacon aptamer is conformationally changed to release the intercalated MB, resulting a decrease in electrical current intensity in voltamogram. The peak signal of the MB is clearly decreased by the binding of thrombin onto the beacon aptamer. The linear range of the signal was observed between 0 and 50.8 nM of thrombin with 0.999 correlation factor. This method was able to linearly and selectively detect thrombin with a detection limit of 11 nM. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16257654/A_novel_electrochemical_detection_method_for_aptamer_biosensors_ DB - PRIME DP - Unbound Medicine ER -