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Detection of pathogen Escherichia coli O157:H7 AT 70 cells/mL using antibody-immobilized biconical tapered fiber sensors.
Biosens Bioelectron. 2005 Dec 15; 21(6):871-80.BB

Abstract

Optical fibers (core diameter 8 microm, cladding diameter 125 microm) was tapered to a waist diameter in the range of 8-12 microm, and then a monoclonal antibody to the pathogen, Escherichia coli O157:H7 was covalently bonded to the surface of the tapered region. Using 470 nm light, the taper was exposed to various concentrations (7 x 10(7), 7 x 10(5), 7 x 10(3), and 70 cells/mL) of the pathogen, and the sensor showed changes in transmitted light as the antigen attached to the antibody on the taper surface. The response was equal and opposite when the pathogen was released from the surface using a low pH buffer. The magnitude of the change was inversely proportional to the concentration of the pathogen. The sensor showed good sensitivity at as low a concentration as 70 cells/mL. The antibody-immobilized taper sensor was also exposed to a mixture of the pathogen and a non-pathogenic variant (JM101) at 0%, 50% and 70% by concentration. The sensor showed good selectivity to the pathogenic antigen. A first order attachment kinetic model is proposed to quantify the rate of attachment of pathogen to the sensor surface. The kinetic rate constant (k) of E. coli O157:H7 to the fiber was found to vary in the range of (2.5-6.1) x 10(-9) min(-1) (cells/mL)(-1).

Authors+Show Affiliations

Department of Chemical Engineering, Drexel University, Philadelphia, PA 19104, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16257655

Citation

Rijal, Kishan, et al. "Detection of Pathogen Escherichia Coli O157:H7 at 70 cells/mL Using Antibody-immobilized Biconical Tapered Fiber Sensors." Biosensors & Bioelectronics, vol. 21, no. 6, 2005, pp. 871-80.
Rijal K, Leung A, Shankar PM, et al. Detection of pathogen Escherichia coli O157:H7 AT 70 cells/mL using antibody-immobilized biconical tapered fiber sensors. Biosens Bioelectron. 2005;21(6):871-80.
Rijal, K., Leung, A., Shankar, P. M., & Mutharasan, R. (2005). Detection of pathogen Escherichia coli O157:H7 AT 70 cells/mL using antibody-immobilized biconical tapered fiber sensors. Biosensors & Bioelectronics, 21(6), 871-80.
Rijal K, et al. Detection of Pathogen Escherichia Coli O157:H7 at 70 cells/mL Using Antibody-immobilized Biconical Tapered Fiber Sensors. Biosens Bioelectron. 2005 Dec 15;21(6):871-80. PubMed PMID: 16257655.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of pathogen Escherichia coli O157:H7 AT 70 cells/mL using antibody-immobilized biconical tapered fiber sensors. AU - Rijal,Kishan, AU - Leung,Angela, AU - Shankar,P Mohana, AU - Mutharasan,Raj, PY - 2004/12/13/received PY - 2005/01/28/revised PY - 2005/02/08/accepted PY - 2005/11/1/pubmed PY - 2006/3/10/medline PY - 2005/11/1/entrez SP - 871 EP - 80 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 21 IS - 6 N2 - Optical fibers (core diameter 8 microm, cladding diameter 125 microm) was tapered to a waist diameter in the range of 8-12 microm, and then a monoclonal antibody to the pathogen, Escherichia coli O157:H7 was covalently bonded to the surface of the tapered region. Using 470 nm light, the taper was exposed to various concentrations (7 x 10(7), 7 x 10(5), 7 x 10(3), and 70 cells/mL) of the pathogen, and the sensor showed changes in transmitted light as the antigen attached to the antibody on the taper surface. The response was equal and opposite when the pathogen was released from the surface using a low pH buffer. The magnitude of the change was inversely proportional to the concentration of the pathogen. The sensor showed good sensitivity at as low a concentration as 70 cells/mL. The antibody-immobilized taper sensor was also exposed to a mixture of the pathogen and a non-pathogenic variant (JM101) at 0%, 50% and 70% by concentration. The sensor showed good selectivity to the pathogenic antigen. A first order attachment kinetic model is proposed to quantify the rate of attachment of pathogen to the sensor surface. The kinetic rate constant (k) of E. coli O157:H7 to the fiber was found to vary in the range of (2.5-6.1) x 10(-9) min(-1) (cells/mL)(-1). SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16257655/Detection_of_pathogen_Escherichia_coli_O157:H7_AT_70_cells/mL_using_antibody_immobilized_biconical_tapered_fiber_sensors_ DB - PRIME DP - Unbound Medicine ER -