Tags

Type your tag names separated by a space and hit enter

Rapid PCR confirmation of E. coli O157:H7 after evanescent wave fiber optic biosensor detection.
Biosens Bioelectron. 2005 Dec 15; 21(6):881-7.BB

Abstract

Escherichia coli O157:H7 is an enteric pathogen of public health importance, which is monitored by several government agencies. Many rapid detection tests have been developed to identify foodstuff and water supplies contaminated by E. coli O157:H7. However, these methods can be time consuming (24-48 h) due to the need to culture the bacteria to confirm detection results. Fiber optic biosensors can rapidly detect pathogens from complex matrices, yet confirmation tests can take up to 10h to complete. In addition, fiber optic biosensors can also be used to reduce the impact of PCR inhibitors present in complex matrices such as food and water. This paper presents methodologies to reduce the time necessary for confirmation from 10 to about 2 h, by direct PCR of bacteria from the fiber optic waveguides without the need for culture or enrichment steps.

Authors+Show Affiliations

Simpson JM
University of South Florida, Department of Biology, Center for Biological Defense, 4202 E. Fowler Ave. SCA110, Tampa, 33620-5200, USA. jsimpson@cas.usf.edu
Lim DV
No affiliation info available

MeSH

Antibodies, BacterialBiosensing TechniquesCell SeparationCoated Materials, BiocompatibleColony Count, MicrobialComputer SystemsEquipment DesignEquipment Failure AnalysisEscherichia coli O157Fiber Optic TechnologyImmunoassayOptical FibersPhotometryPolymerase Chain ReactionReproducibility of ResultsSensitivity and SpecificitySystems Integration

Pub Type(s)

Evaluation Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16257656

Citation

Simpson, Joyce M., and Daniel V. Lim. "Rapid PCR Confirmation of E. Coli O157:H7 After Evanescent Wave Fiber Optic Biosensor Detection." Biosensors & Bioelectronics, vol. 21, no. 6, 2005, pp. 881-7.
Simpson JM, Lim DV. Rapid PCR confirmation of E. coli O157:H7 after evanescent wave fiber optic biosensor detection. Biosens Bioelectron. 2005;21(6):881-7.
Simpson, J. M., & Lim, D. V. (2005). Rapid PCR confirmation of E. coli O157:H7 after evanescent wave fiber optic biosensor detection. Biosensors & Bioelectronics, 21(6), 881-7.
Simpson JM, Lim DV. Rapid PCR Confirmation of E. Coli O157:H7 After Evanescent Wave Fiber Optic Biosensor Detection. Biosens Bioelectron. 2005 Dec 15;21(6):881-7. PubMed PMID: 16257656.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid PCR confirmation of E. coli O157:H7 after evanescent wave fiber optic biosensor detection. AU - Simpson,Joyce M, AU - Lim,Daniel V, PY - 2004/11/10/received PY - 2005/02/11/revised PY - 2005/02/15/accepted PY - 2005/11/1/pubmed PY - 2006/3/10/medline PY - 2005/11/1/entrez SP - 881 EP - 7 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 21 IS - 6 N2 - Escherichia coli O157:H7 is an enteric pathogen of public health importance, which is monitored by several government agencies. Many rapid detection tests have been developed to identify foodstuff and water supplies contaminated by E. coli O157:H7. However, these methods can be time consuming (24-48 h) due to the need to culture the bacteria to confirm detection results. Fiber optic biosensors can rapidly detect pathogens from complex matrices, yet confirmation tests can take up to 10h to complete. In addition, fiber optic biosensors can also be used to reduce the impact of PCR inhibitors present in complex matrices such as food and water. This paper presents methodologies to reduce the time necessary for confirmation from 10 to about 2 h, by direct PCR of bacteria from the fiber optic waveguides without the need for culture or enrichment steps. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16257656/Rapid_PCR_confirmation_of_E__coli_O157:H7_after_evanescent_wave_fiber_optic_biosensor_detection_ DB - PRIME DP - Unbound Medicine ER -