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Variations in CYP74B2 (hydroperoxide lyase) gene expression differentially affect hexenal signaling in the Columbia and Landsberg erecta ecotypes of Arabidopsis.
Plant Physiol. 2005 Nov; 139(3):1529-44.PP

Abstract

The CYP74B2 gene in Arabidopsis (Arabidopsis thaliana) ecotype Columbia (Col) contains a 10-nucleotide deletion in its first exon that causes it to code for a truncated protein not containing the P450 signature typical of other CYP74B subfamily members. Compared to CYP74B2 transcripts in the Landsberg erecta (Ler) ecotype that code for full-length hydroperoxide lyase (HPL) protein, CYP74B2 transcripts in the Col ecotype accumulate at substantially reduced levels. Consistent with the nonfunctional HPL open reading frame in the Col ecotype, in vitro HPL activity analyses using either linoleic acid hydroperoxide or linolenic acid hydroperoxide as substrates show undetectable HPL activity in the Col ecotype and C6 volatile analyses using leaf homogenates show substantially reduced amounts of hexanal and no detectable trans-2-hexenal generated in the Col ecotype. P450-specific microarrays and full-genome oligoarrays have been used to identify the range of other transcripts expressed at different levels in these two ecotypes potentially as a result of these variations in HPL activity. Among the transcripts expressed at significantly lower levels in Col leaves are those coding for enzymes involved in the synthesis of C6 volatiles (LOX2, LOX3), jasmonates (OPR3, AOC), and aliphatic glucosinolates (CYP83A1, CYP79F1, AOP3). Two of the three transcripts coding for aliphatic glucosinolates (CYP83A1, AOP3) are also expressed at significantly lower levels in Col flowers.

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Authors+Show Affiliations

Duan H
Department of Cell and Developmental Biology, University of Illinois, Urbana, IL 61801, USA.
Huang MY
No affiliation info available
Palacio K
No affiliation info available
Schuler MA
No affiliation info available

MeSH

Aldehyde-LyasesAmino Acid SequenceArabidopsisBase SequenceChromatography, GasCytochrome P-450 Enzyme SystemDNA, ComplementaryGene Expression ProfilingGene Expression Regulation, PlantHexobarbitalMolecular Sequence DataOligonucleotide Array Sequence AnalysisPolymorphism, GeneticRNA, MessengerSequence AlignmentSignal TransductionVolatilization

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16258015

Citation

Duan, Hui, et al. "Variations in CYP74B2 (hydroperoxide Lyase) Gene Expression Differentially Affect Hexenal Signaling in the Columbia and Landsberg Erecta Ecotypes of Arabidopsis." Plant Physiology, vol. 139, no. 3, 2005, pp. 1529-44.
Duan H, Huang MY, Palacio K, et al. Variations in CYP74B2 (hydroperoxide lyase) gene expression differentially affect hexenal signaling in the Columbia and Landsberg erecta ecotypes of Arabidopsis. Plant Physiol. 2005;139(3):1529-44.
Duan, H., Huang, M. Y., Palacio, K., & Schuler, M. A. (2005). Variations in CYP74B2 (hydroperoxide lyase) gene expression differentially affect hexenal signaling in the Columbia and Landsberg erecta ecotypes of Arabidopsis. Plant Physiology, 139(3), 1529-44.
Duan H, et al. Variations in CYP74B2 (hydroperoxide Lyase) Gene Expression Differentially Affect Hexenal Signaling in the Columbia and Landsberg Erecta Ecotypes of Arabidopsis. Plant Physiol. 2005;139(3):1529-44. PubMed PMID: 16258015.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Variations in CYP74B2 (hydroperoxide lyase) gene expression differentially affect hexenal signaling in the Columbia and Landsberg erecta ecotypes of Arabidopsis. AU - Duan,Hui, AU - Huang,Meng-Yu, AU - Palacio,Kathryn, AU - Schuler,Mary A, Y1 - 2005/10/28/ PY - 2005/11/1/pubmed PY - 2006/2/10/medline PY - 2005/11/1/entrez SP - 1529 EP - 44 JF - Plant physiology JO - Plant Physiol VL - 139 IS - 3 N2 - The CYP74B2 gene in Arabidopsis (Arabidopsis thaliana) ecotype Columbia (Col) contains a 10-nucleotide deletion in its first exon that causes it to code for a truncated protein not containing the P450 signature typical of other CYP74B subfamily members. Compared to CYP74B2 transcripts in the Landsberg erecta (Ler) ecotype that code for full-length hydroperoxide lyase (HPL) protein, CYP74B2 transcripts in the Col ecotype accumulate at substantially reduced levels. Consistent with the nonfunctional HPL open reading frame in the Col ecotype, in vitro HPL activity analyses using either linoleic acid hydroperoxide or linolenic acid hydroperoxide as substrates show undetectable HPL activity in the Col ecotype and C6 volatile analyses using leaf homogenates show substantially reduced amounts of hexanal and no detectable trans-2-hexenal generated in the Col ecotype. P450-specific microarrays and full-genome oligoarrays have been used to identify the range of other transcripts expressed at different levels in these two ecotypes potentially as a result of these variations in HPL activity. Among the transcripts expressed at significantly lower levels in Col leaves are those coding for enzymes involved in the synthesis of C6 volatiles (LOX2, LOX3), jasmonates (OPR3, AOC), and aliphatic glucosinolates (CYP83A1, CYP79F1, AOP3). Two of the three transcripts coding for aliphatic glucosinolates (CYP83A1, AOP3) are also expressed at significantly lower levels in Col flowers. SN - 0032-0889 UR - https://www.unboundmedicine.com/medline/citation/16258015/Variations_in_CYP74B2__hydroperoxide_lyase__gene_expression_differentially_affect_hexenal_signaling_in_the_Columbia_and_Landsberg_erecta_ecotypes_of_Arabidopsis_ DB - PRIME DP - Unbound Medicine ER -