Tags

Type your tag names separated by a space and hit enter

Molecular cytogenetic findings of acute leukemia included in the Brazilian Collaborative Study Group of Infant acute leukemia.
Pediatr Blood Cancer. 2006 Oct 15; 47(5):549-54.PB

Abstract

BACKGROUND

Chromosome abnormalities often occur prenatally in childhood leukemia, characterizing an early event in leukemogenesis. The majority of the abnormalities occurring in infants involve the MLL gene on chromosome band 11q23. We describe the molecular cytogenetic findings of 207 infant acute leukemia (IAL) cases included in the Brazilian Collaborative Study Group of Infant acute leukemia.

PROCEDURE

The diagnosis of Acute Lymphoblastic leukemia (ALL) or acute myeloblastic leukemia (AML) was made according to morphology and immunophenotyping classification, followed by conventional karyotyping. Samples were then screened using RT-PCR for the presence of specific chromosome translocations. FISH assay for MLL rearrangements was performed only in cases with negative or inconclusive cytogenetic or PCR results.

RESULTS

The characteristics of children with IAL were as follows: 115 boys and 92 girls, age range 0-23 months, mean age 12 months, 145 ALL, and 62 AML. A statistically significant association was observed between pro-B ALL cases and MLL+ve (P=0.0001) cases and the age group 0-3 months with MLL+ve (P=0.008) cases. Two rare cases of pro-T ALL with MLL+ve were found. Other than MLL rearrangements, various other molecular aberrations were detected including TEL/AML1+ve (n=9), E2A/PBX1+ve (n=4), PML/RARA+ve (n=4), and AML1/ETO+ve (n=2). Cytogenetic analysis revealed hyperdiploidy (n=6), del(7) in two cases and del(11)(q23) in seven cases.

CONCLUSIONS

Our results show that not only MLL rearrangements, but also other molecular abnormalities occur before birth and may contribute to leukemogenesis.

Authors+Show Affiliations

Divisão de Medicina Experimental, Coordenação de Pesquisa, Instituto Nacional de Câncer, Rio de Janeiro, RJ, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16261608

Citation

Emerenciano, Mariana, et al. "Molecular Cytogenetic Findings of Acute Leukemia Included in the Brazilian Collaborative Study Group of Infant Acute Leukemia." Pediatric Blood & Cancer, vol. 47, no. 5, 2006, pp. 549-54.
Emerenciano M, Agudelo Arias DP, Coser VM, et al. Molecular cytogenetic findings of acute leukemia included in the Brazilian Collaborative Study Group of Infant acute leukemia. Pediatr Blood Cancer. 2006;47(5):549-54.
Emerenciano, M., Agudelo Arias, D. P., Coser, V. M., de Brito, G. D., Macedo Silva, M. L., & Pombo-de-Oliveira, M. S. (2006). Molecular cytogenetic findings of acute leukemia included in the Brazilian Collaborative Study Group of Infant acute leukemia. Pediatric Blood & Cancer, 47(5), 549-54.
Emerenciano M, et al. Molecular Cytogenetic Findings of Acute Leukemia Included in the Brazilian Collaborative Study Group of Infant Acute Leukemia. Pediatr Blood Cancer. 2006 Oct 15;47(5):549-54. PubMed PMID: 16261608.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cytogenetic findings of acute leukemia included in the Brazilian Collaborative Study Group of Infant acute leukemia. AU - Emerenciano,Mariana, AU - Agudelo Arias,Diana Patricia, AU - Coser,Virginia Maria, AU - de Brito,Gilena Dantas, AU - Macedo Silva,Maria L, AU - Pombo-de-Oliveira,Maria S, AU - ,, PY - 2005/11/2/pubmed PY - 2006/12/14/medline PY - 2005/11/2/entrez SP - 549 EP - 54 JF - Pediatric blood & cancer JO - Pediatr Blood Cancer VL - 47 IS - 5 N2 - BACKGROUND: Chromosome abnormalities often occur prenatally in childhood leukemia, characterizing an early event in leukemogenesis. The majority of the abnormalities occurring in infants involve the MLL gene on chromosome band 11q23. We describe the molecular cytogenetic findings of 207 infant acute leukemia (IAL) cases included in the Brazilian Collaborative Study Group of Infant acute leukemia. PROCEDURE: The diagnosis of Acute Lymphoblastic leukemia (ALL) or acute myeloblastic leukemia (AML) was made according to morphology and immunophenotyping classification, followed by conventional karyotyping. Samples were then screened using RT-PCR for the presence of specific chromosome translocations. FISH assay for MLL rearrangements was performed only in cases with negative or inconclusive cytogenetic or PCR results. RESULTS: The characteristics of children with IAL were as follows: 115 boys and 92 girls, age range 0-23 months, mean age 12 months, 145 ALL, and 62 AML. A statistically significant association was observed between pro-B ALL cases and MLL+ve (P=0.0001) cases and the age group 0-3 months with MLL+ve (P=0.008) cases. Two rare cases of pro-T ALL with MLL+ve were found. Other than MLL rearrangements, various other molecular aberrations were detected including TEL/AML1+ve (n=9), E2A/PBX1+ve (n=4), PML/RARA+ve (n=4), and AML1/ETO+ve (n=2). Cytogenetic analysis revealed hyperdiploidy (n=6), del(7) in two cases and del(11)(q23) in seven cases. CONCLUSIONS: Our results show that not only MLL rearrangements, but also other molecular abnormalities occur before birth and may contribute to leukemogenesis. SN - 1545-5009 UR - https://www.unboundmedicine.com/medline/citation/16261608/Molecular_cytogenetic_findings_of_acute_leukemia_included_in_the_Brazilian_Collaborative_Study_Group_of_Infant_acute_leukemia_ L2 - https://doi.org/10.1002/pbc.20654 DB - PRIME DP - Unbound Medicine ER -